Browse > Article
http://dx.doi.org/10.5187/JAST.2002.44.4.407

Utilization of Plant Phytase to Improve Phosphorous Availability for Broiler  

Kim, B.H. (Department of Animal Science, College of Industrial Science, Chung-Ang Uniyersity)
Namkung, H. (Department of Animal Science, College of Industrial Science, Chung-Ang Uniyersity)
Paik, I.K. (Department of Animal Science, College of Industrial Science, Chung-Ang Uniyersity)
Publication Information
Journal of Animal Science and Technology / v.44, no.4, 2002 , pp. 407-418 More about this Journal
Abstract
This study was conducted to evaluate the efficacy of wheat and wheat bran  as the source of phytase in a 5 week broiler feeding trial. One thousand day-old broiler chickens(Ross$^{(R)}$) were divided into 20 pens of 50 broilers(25 male and 25 female) each. Four pens were randomly arranged to one of the five dietary treatments: T1, control diet containing normal nonphytate P(NPP) ;  T2, T1 － 0.1% NPP; T3, T2 ＋ 600IU microbial phytase(NOVO$^{(R)}$) per kg diet; T4, T2 ＋ 600IU plant phytase from wheat and wheat bran; T5, T2 ＋ 600IU plant phytase from wheat and hydrothermally treated wheat bran. Reduction of NPP level by 0.1%(T2) reduced weight gain and feed intake but plant phytase treatments(T4 and T5) recovered the lost performance. Plant phytase treatments showed better (p<0.05) weight gain and intake than the microbial phytase treatment(T3). There was no difference between regular wheat bran treatment(T4) and hydrothermally treated wheat bran treatment(T5). Mortality was the highest by low NPP diet(T2). Availability of ether extract and crude ash of grower diet was the highest(p<0.05) in normal wheat bran diet(T4). Availability of Ca and P of grower diet was the highest(p<0.05) in T4 followed by T3 and T5. Availability of Mg, Fe and Zn was drastically improved by phytase treatments(T3, T4 and T5). Excretion of Ca, P, Mg, Fe and Zn was the lowest(p<0.05) with microbial phytase treatment(T3). Serum level of Ca and Mg was the highest(p<0.05) with the low NPP treatment(T2). Tibial ash content of T2 and T3 was lower(p<0.05) than that of T1, T4 and T5. However, tibial Ca content was higher(p<0.05) in T1 and T2 than other treatments. Tibial P and Mg contents were the highest(p<0.05) in T1. It was concluded that plant phytase from wheat bran can be effectively used to improve P utilization. Hydrothermal treatment of wheat bran prior to inclusion in the diet had no beneficial effects.
Keywords
Broiler; Wheat; Wheat bran; Plant phytase; Microbial phytase; Phosphorus;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Mitchell, R. D. and Edwards, H. M. JR. 1996. Additive effects of 1,25-Dihydroxycholecalciferol and phytase on phytate phosphorus utilization and related parameters in broiler chickens. Poultry Sci. 75:111-119.
2 Pointillart, A. 1991. Enhancement of phosphorus utilization in growing pigs, fed  phytate-rich diets by using rye bran. J. Anim. Sci. 69:1109-1115.
3 Pointillart, A. 1993. Importance of phytates and cereal phytases in the feeding of pig. Enzymes in Animal Nutrition. Ibid. pp.192-198.
4 Qian, H. E., Kornegray, T. and Dendow, D. M.. 1996. Phosphorus equivalence of microbial phytase in turkey diets as influenced by calcium to phosphorus ratios and phosphorus levels. Poultry Sci. 75:69-81.
5 권 관, 권찬호, 장재익, 주종철, 유문일, 손광수, 최양웅. 1999 옥수수－대두박 사료내 미생물 phytase의 첨가가 브로일러의 생산능력에 미치는 영향. 한축지 41(5):519-526
6 이선재, 엄재상, 남궁환, 백인기. 1999. 식물성 사료원료내 피틴대 인의 함량과 Phytase Activity 및 그 특성에 관한 연구. 한영사지. 23(6):501- 506.
7 Haug, W. and Lantzsch, H. J. 1983. Sensitive method for the rapid determination of phytate in cereals and aereal products. J. Sci. Food Agric. 34:1423-1426.   DOI
8 Eeckhout, W. and De Paepe, M. 1992. Effects of phytase from wheat or triticale, and Ca level of a pig feed, on P digestibility with pigs. 4th Int. Conf.  IMPHOS, Ghent, 8-11 September 1992. Instit Mondial du Phosphate, Casablanca, p. 66, Abstract.
9 Fretzdorff, B., Brummer, J. M., Rocken, W., Greiner, R., Konietzny, U. and Jany, K. 1995. Reduktion des phytinsaure-gehaltes bei der herstellung von Backwaren and getreidenahr- mitteln. AID - Verbraucherdienst. 40:12-20.
10 Han, Y. M., Yang, F., Zhou, A. G., Miller, E. R., Ku, P. K., Hogberg, M. G. and Lei, X. G. 1997. Supplemental phytases of microbial and cereal sources improve dietary phytate phos- phorous utilization by pigs from weaning through finishing. J. Anim. Sci. 75:1017-1025.   DOI
11 IUPAC-IUB: Comission on Biochemical Nomen- clature. 1977. Nomenclature of phosphorus con- taining compounds of biochemical importance. Eur. J. Biochem. 79:1-9.
12 Kemme, P. A. and Jongbloed, A. W. 1989. Effect van tarwe phytase op de Ca- en P-verteerbaarheid onder involed van maalfijnheid, voorweken en pelleteren. Communication 202, IVVO, Lelystad, Netherlands.
13 Lim, H. S., Namkung, H., Um, J. S., Kang, K. R., Kim, B. S. and Paik, I. K. 2001. The effects of phytase supplementation on the performance of broiler chickens fed dicts with different levels of non-phytate phosphorus. 14(2):250-257.
14 Sebastian, S., Touchburn, S. P., Chavez, E. R. and Lague, P. C. 1996b. Effiacay of supplemental microbial phytase at different dietry calcium levels on growth performance and mineral utilization of broiler chickens. Poultry Sci. 75:1516-1523.
15 Ravindran V., Denbow, D. M., Kornegay, E. T., Yi, Z. and Hulet, R. J. 1995. Supplemental phytase improves availablity of phosphorus in soybean meal for turkey poults. Poultry Sci, 74: 1843-1854.   DOI
16 Sebastian, S., Touchburn, S. P. and Chavez, E. R. 1998. Implication of phytic  acid and supplemental microbial phytase in poultry nutrition : a review. World's Poultry Sci. J. 54:27-47.
17 Sebastian, S., Touchburn, S. P., Chavez, E. R. and Lague, P. C. 1996a. The effects of supple- mental Microbial phytase on the performance and utilization of dietary calcium, phosphorus, copper, and zinc in broiler chickens fed corn-soybean diets. Poultry Sci. 75:729-736.
18 Taylor, T. G. and Dacke, C. G. 1984. Calcium metabolism and its regulation. Pages 126-170. in: Physiology and biochemistry of the domestic fowl. B. M. Freeman, ed. Academic Press, London, UK.
19 Yi. Z., Kornegay. E. T., Ravindran. V. and Cenbow, D. M. 1996. Improving phytate phos- phurus availablility in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase. Poultry Sci. 75:240-249.   DOI
20 Zhang, Z. B., Kornegay, E. T., Radcliffe, J. S., Dendow, D. M., Veit, H. P. and Larsen, C. T. 2000. Comparison of genetically engineered microbial and plant phytase for young broilers. Poultry Sci. 79:709-717.   DOI
21 Association of Offical Analytical Chemist, 1990. Official Methods of Analysis. 15thed. Association of Offical Analytical Chemists, Arlington, VA.
22 Eeckhout, W. and De Paepe, M. 1994. Total phosphorus, phytate-phosphorus and phytase acti- vity in plant feedstuffs. Anim. Feed Sci. Tech. 47:19-29.
23 De Rham, O. and Joost, T. 1979. Phytate protein interaction in soybean extracts and low-phytate soy protein products. J. Food Sci. 44:596-600.
24 Eeckhout, W. and De Paepe, M. 1991. The quantitative effects of an industrial microbial phytase and wheat phytase on the apparent phosphorus absorbability of a mixed feed by piglets. Meded. Fac. Landbouwwet. Rijksuniv. Gent, 56:1643-1648.