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Analysis on Artificial Insemination Failure and Characteristics of Frozen Semen Used for Reproduction of Hanwoo Cow in Gangwon East Area  

Park, Sai-Rom (College of Animal Life Science, Kangwon National University)
Hong, Min-Wook (College of Animal Life Science, Kangwon National University)
Kim, Hun (College of Animal Life Science, Kangwon National University)
Lee, Seung-Kyu (College of Animal Life Science, Kangwon National University)
Lee, Yeung-Sub (College of Animal Life Science, Kangwon National University)
Kim, Jin-Woo (College of Animal Life Science, Kangwon National University)
Lee, Hak-Kyo (Department of Biotechnology, Hanyong National University)
Jeong, Dong-Kee (Department of Biotechnology, Jeju National University)
Kim, Jong-Bok (College of Animal Life Science, Kangwon National University)
Song, Young-Han (College of Animal Life Science, Kangwon National University)
Lee, Sung-Jin (College of Animal Life Science, Kangwon National University)
Publication Information
Reproductive and Developmental Biology / v.36, no.1, 2012 , pp. 27-32 More about this Journal
Abstract
This study was carried out to investigate artificial insemination (AI) failure status and frozen semen characteristics in Korean proven bulls' number (KPN) semen used for AI of Hanwoo cows in Gangwon East region (Gangneung, Donghae, Taebaek, Samcheok, Sokcho, Yangyang, Goseong). Among semen used for AI, AI failure rate showed lowest at KPN506 (27.6%), whereas highest at KPN593 (77.2%). Correlations of AI failure in between Korean proven bulls semen and cows was 0.2941, which means that AI failure rate of Korean proven bulls semen may have respectable effect on reproduction of Hanwoo cow. In addition, present study was conducted to investigate spermatozoal viability rate, ruptured acrosome rate and active mitochondria in frozen Korean proven bulls semen with flow cytometry. The semen of KPN593 showed significantly ($p$<0.05) higher viability rate in KPN593 (30.49%) than that in KPN637 (37.34%). Furthermore, percentage of ruptured acrosome was lower in KPN637 as 21.37% than in KPN637 (21.37%), but it was not statistically significant. In conclusion, these results indicate that choice of Korean proven bulls semen may correlate positively with conception rate in Hanwoo cow. Therefore, KPN with high AI failure rate might be avoid to increase conception rate and characteristics of frozen semen might be evaluated before its use for AI.
Keywords
Artificial insemination; KPN semen; Hanwoo cows; Flow-cytometry;
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Times Cited By KSCI : 2  (Citation Analysis)
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1 이성수, 노승희, 박노형, 원유석 (2010a): 한우 씨수소의 고환 둘레와 정액 성상 및 농가 인공수정 수태율과의 관계. J Emb Trans 25(1):51-55.
2 이성수, 노승희, 박노형, 원유석 (2010b): 한우 씨수소의 상대적 수정능 추정에 관한 연구. J Emb Trans. 25(1):57-66.
3 박노형, 이성수, 정준, 원유석, 김내수 (2003): 한우 종모우의 고환둘레와 정액생산 및 번식과의 관계. J Anim Sci & Technol 45(4):517-522.
4 박새롬, 이성국, 이규수, 신영수, 송영한, 이성진 (2011): 강원 영동지방 한우암소 번식 및 육종현황 분석. 동물자원연구. 22(1):1-5.
5 Curry MR (2002): Cryopreservation of semen from domestic livestock. Rev Reprod 5:46-52.
6 Hammerstedt RH, Graham JK, Nolan JP (1990): Cryopreservation of mammalian sperm: What we ask them to survive. J Androl 11:73-88.
7 Medeiros CM, Forell F, Oliveira AT, Rodrigues JL (2002): Current status of sperm cryopreservation: why isn't it better? Theriogenology 57:3 27-344.   DOI
8 Murray BB, Scheffer LR, Burnside EB (1983): Heritability of nonreturn rate of Canadian Holstein-Friesian bulls. Can J Anim Sci 63:39-48.   DOI
9 Nadarajah K, Burnside EB, Scheffer LR (1988): Genetic parameters for fertility of dairy bulls. J Dairy Sci 71:2730-2734.   DOI
10 Stälhammer EM, Janson L, Philipsson J (1994): Genetic studies on fertility in A.I. bulls. II. Environmental and genetic effects on non-return rate of young bulls. Anim Reprod Sci 34:193-207.   DOI
11 Thaller G (1997): Genetics and breeding for fertility. Interbull Mtg Grub Uppsala Interbull Bull No 18.
12 Clay JS, McDaniel BT, Brown CH (2004): Variances of and correlation among progeny tests for reproductive traits of cows sired by AI bulls. J Dairy Sci 87:2307-2313.   DOI
13 Thundathil J, Gil J, Januskauskas A, Larsson B, Soderquist L, Mapletoft R, Rodriguez-Martinez H (1999): Relationship between thd proportion of capacitated spermatozoa present in frozen-thawed bull semen and fertility with artificial insemination. Int J Androl 22:366-373.   DOI
14 Weigel KA (2006): Prospects for improving reproductive performance through genetic selection. Animal Reproduction Science 96:323-330.   DOI