Browse > Article

Isolation and Identification of Pathogenic Bacteria from Salads of Fast Food Restaurants  

Park Yong-Bae (North branch kyonggi-do Institute of Health & Environment)
Kang Jeong-Bok (North branch kyonggi-do Institute of Health & Environment)
Kim Jung-Beom (North branch kyonggi-do Institute of Health & Environment)
Kim Jong-Chan (North branch kyonggi-do Institute of Health & Environment)
Publication Information
Journal of environmental and Sanitary engineering / v.20, no.1, 2005 , pp. 23-31 More about this Journal
Abstract
On the purpose of epidemiological survey relate to food poisoning, a total of 114 samples of different salads collected from fast food Restaurants in Gyeonggi-do were for the presence of pathogenic microorganisms. Microbial assessment of salads revealed that TPC($1.1\times10\;\~\;8.4\times10^{5}\;CFU/g$) and coliforms($0\~5.4\times10^{4}\;CFU/g$) exceeded the standards by Solberg et al. ($TPC:10^{5}\;CFU/g,\;coliforms:10^{2}\;CFU/g$). Two pathogenic bacteria were isolated from salad samples, and identified by biochemical methods, including API identification systems. Isolates from PALCAM agar and MYP agar media were in 98.6, $99.8\%$ agreements with Listeria monocytogenes, Bacillus cereus at the species level, respectively. All 7 strains of Bacillus cereus isolates produced enterotoxin as revealed with CRET-RPLA.
Keywords
salad; pathogenic bacteria; identification; CRET-RPLA;
Citations & Related Records
연도 인용수 순위
  • Reference
1 임순영, 윤석권 냉동식품에서 분리한 Yersinia enterocolitica의 특성. 한국식품과학회지 31(6), 1336. 2000   과학기술학회마을
2 김기욱. 한국식품산업의 프렌차이징 전략-국내진출 패스트푸드 중심으로. 중앙대학교 국제경영대학원 석사학위논문. 1992
3 Gillian, A. F., Christopher, T., and David, O. The micro biological safety of minimally processed vegetables. Int. J. Food sci. Technol. 34, 1 22. 1999   DOI   ScienceOn
4 Swerdlow, D. L., and Altekruse, S. F. Food-borne diseases in the Global Village. Emerging Infections 2. Scheld, W. M., Craig, W .A. and Hughes, J. M. (eds). American Society for Microbiology, Washington. 273-294. 1998
5 Kalish, F. Extending the HACCP concept to product distri- bution. Food Technol, 45, 119. 1991
6 NACMCF HACCP princip-les for the food production USDA, FSIS. Washington D.C. 1989
7 식품공전 식품의약품안전청 78-112. 2000
8 감염병실험실 진단지침 국립보건원. 109-283. 1996
9 Levin MM. Escherichia coli that cause diarrhea, enterotoxigenic, enteropathogenic, enteroinvasive, enterohaemorrhagic, enteroadherent. J. Infect. Dis. 155. 337-389. 1987
10 WHO. Food borne Listeriosis. Document No. WHO / WHE / FOS / 88.5. World Health organization, Geneva, Switzerland. 1988
11 Choma, C, Guinebretiere, M. H., Carlin, F., Schmitt, P., Velge, P., Granum, P. E. and Nguyenthe, C. Prevalence character- ization and growth of Bacillus cereus in commercial cooked chilled foods containing vegetables. J. Appl. Microbial. 88. 617-625. 2000.   DOI   ScienceOn
12 Solberg, M., Buckalew, J.J., Chen, C.M., Schaffner, D.W., O'Neill, k, McDowell J., Post, L. S., and Borderck, M. Mcro- biological safety assurance system for food service facilities. Food Technol. 44, 68. 1990
13 Seeligeri, H.P.R. and Finger, H. In J.S. Remington and J.O. klein(eds). Infectious disease of the fetus and newborne infant, 2nd ed. W.B. Saunders, Philadelphia. 264-289. 1983
14 식품의약품안전청. 식중독 발생현황 및 예방대책. 2004
15 William H. Ewing, Edwards and Ewing's Identification of Entero-bacteriaceae 4th ed. Elsevier science publishing co., Inc. New York. 93-134. 1986
16 Carlin, F., Guinebretiere, M. H., Choma, C, Pasgualini, R., Braco nnier, A. and Nguyenthe, C. Sporeforming bacteria in commercial cooked, pasteurized and chilled vegetable purees. Food Microbial. 17.153-165. 2000   DOI   ScienceOn
17 강영재 HACCP란 무엇인가. 식품과학과산업 26, 4. 1993
18 박석기 등 위생 미생물 시험법 해설. 미래문화 60 72. 1998
19 전국경제인연합 한국경제연감. 2000
20 이용욱, 홍종해 우리나라에서 보고된 집단 식중독의 발생특징. 식품공업, 109. 1997
21 오덕환 최소가공야채류의 미생물학적 안전성. 식품산업과영양 4(3), 48-54. 1999   과학기술학회마을
22 Schofield, G. M. Emerging foodborne Pathogens and their significance in chilled foods. J. Appl. Bacteriol. 72, 267-273. 1992   DOI   PUBMED
23 곽동경, 류은순. 패스트푸드 업체의 급식관리 구조개선을 위한 모형설정에 관한 연구. 한국식문화학회지 5(4), 456-463. 1990   과학기술학회마을
24 Tsai, Y., palmer, C. J.m and Sangermano, L. R. Detection of Escherichia coli in sewage and sludge by polymerase chain reaction. Appl. Environ Microbial. 59. 353-357. 1992
25 양재승 식품의안전성과 HACCP 식품과학과산업. 30, 172-182. 1997
26 Beattie, S.H. and Williams, A.G. Detection of toxigenic strains Bacillus cereus and other Bacillus spp. with an improved cytotoxicity assay. Lett. Appl. Microbial. 28. 221-225. 1999   DOI   ScienceOn