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http://dx.doi.org/10.4196/kjpp.2022.26.5.313

Protective effect of low-intensity treadmill exercise against acetylcholine-calcium chloride-induced atrial fibrillation in mice  

Sung, Dong-Jun (Department of Sport and Health Studies, College of Biomedical and Health Science, Konkuk University)
Jeon, Yong-Kyun (Department of Physical Education at the Graduate School of Education, Dankook University)
Choi, Jaeil (Department of Physical Education at the Graduate School of Education, Dankook University)
Kim, Bokyung (Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine)
Golpasandi, Shadi (Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine)
Park, Sang Woong (Department of Emergency Medical Services, College of Health Sciences, Eulji University)
Oh, Seung-Bum (Department of Sport and Health Studies, College of Biomedical and Health Science, Konkuk University)
Bae, Young Min (Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.26, no.5, 2022 , pp. 313-323 More about this Journal
Abstract
Atrial fibrillation (AF) is the most common supraventricular arrhythmia, and it corresponds highly with exercise intensity. Here, we induced AF in mice using acetylcholine (ACh)-CaCl2 for 7 days and aimed to determine the appropriate exercise intensity (no, low, moderate, high) to protect against AF by running the mice at different intensities for 4 weeks before the AF induction by ACh-CaCl2. We examined the AF-induced atrial remodeling using electrocardiogram, patch-clamp, and immunohistochemistry. After the AF induction, heart rate, % increase of heart rate, and heart weight/body weight ratio were significantly higher in all the four AF groups than in the normal control; highest in the high-ex AF and lowest in the low-ex (lower than the no-ex AF), which indicates that low-ex treated the AF. Consistent with these changes, G protein-gated inwardly rectifying K+ currents, which were induced by ACh, increased in an exercise intensity-dependent manner and were lower in the low-ex AF than the no-ex AF. The peak level of Ca2+ current (at 0 mV) increased also in an exercise intensity-dependent manner and the inactivation time constants were shorter in all AF groups except for the low-ex AF group, in which the time constant was similar to that of the control. Finally, action potential duration was shorter in all the four AF groups than in the normal control; shortest in the high-ex AF and longest in the low-ex AF. Taken together, we conclude that low-intensity exercise protects the heart from AF, whereas high-intensity exercise might exacerbate AF.
Keywords
Acetylcholine-calcium chloride; Action potential; Atrial fibrillation; Exercise-intensity; GIRK channels;
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