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Isolation and Culture of Methanotrophs in Inorganic Medium and Characterization of COD Production, Nutrient Removal  

Kim, I-Tae (Korea Institute of Construction Technology)
Bae, Woo-Keun (Hanyang University Department of Civil & Environmental Systems Engineering)
Kim, Kwang-Soo (Korea Institute of Construction Technology)
Lee, Hee-Ja (Korea Institute of Construction Technology)
Publication Information
Journal of Korean Society of Environmental Engineers / v.27, no.11, 2005 , pp. 1198-1204 More about this Journal
Abstract
The objectives of this study were to isolate and culture methanotrophs and to apply them for biological removal of nitrogen and phosphorous. Methanotrophs (dominant species: Methylomonas methanica) were isolated from a landfill cover soil, cultured in a NMS medium, and analyzed to reveal their characteristics of growth and nutrient removal. The methanotrophs themselves can produce substantial amount of organic substances(as COD) including methanol, formaldehyde, and formate, as carbon sources required for denitrification. For instance, the production rate for methanol was $8\;mg/L{\cdot}hr$. Moreover, the analysis of nitrogen and phosphorous in the sludge suggested that the methanotrophs assimilate nitrogen and phosphorous as growth substances.
Keywords
Methanotrophs; COD Production; MMO(Methane Monooxygenase);
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