Browse > Article

Relationship between FSH Receptor Genotype and Clinical Outcomes of IVF-ET in Infertile Korean Women  

Moon, Mi-Hye (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Choi, Hye-Won (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Kim, Min-Jee (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Lee, Hyoung-Song (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Cha, Sun-Hwa (Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Song, In-Ok (Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Koong, Mi-Kyoung (Department of Obstetrics and Gynecology, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Jun, Jin-Hyun (Laboratory of Reproductive Biology & Infertility, Cheil General Hospital & Women's Healthcare Center, Kwandong University College of Medicine)
Publication Information
Clinical and Experimental Reproductive Medicine / v.35, no.1, 2008 , pp. 69-76 More about this Journal
Abstract
Objective: The purposes of this study were to determine the distribution of follicle-stimulating hormone receptor (FSHR) genotypes in infertile Korean women and to evaluate the relationship between FSHR genotypes and clinical outcomes of IVF-ET cycles. Methods: Genomic DNA was extracted from peripheral blood in 1, 020 of infertile Korean women. Genotypes of FSHR at Thr307Ala (T/A) and Asn680Ser (N/S) were screened by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Clinical outcomes related to the genotypes of FSHR were evaluated in IVF-ET cycles (n=302) with controlled ovarian hyperstimulation (COH) of infertile women under 40 years old. Results: In a population of 1, 020 infertile Korean women, the frequency of TT/NN, TA/NS and AA/SS for the major variant Thr307Ala and Asn680Ser was 44.80%, 41.96% and 10.49%, respectively. There was no significant difference in characteristics of ovarian response and clinical pregnancy rate among the major genotypes of FSHR in IVF-ET cycles with COH. However, implantation rate of AA/SS patients was significantly higher than that of TT/NN patients (24.5% vs 15.7%, p<0.05). Conclusion: This study showed that FSHR genotype was not directly associated with ovarian response in IVF-ET cycles with COH. The relationship between clinical outcomes and FSHR genotypes of patients should be substantiated by further studies.
Keywords
Follicle-stimulating hormone receptor (FSHR); Genotype; Controlled ovarian hyperstimulation (COH); Ovarian response; Clinical outcomes;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Wheeler C, Ben-Rafael Z, Benjamini Y, Blasco L, Flickinger G, Tureck RW, et al. Repetitive ovarian response to gonadotropin stimulation in an in vitro fertilization program. Gynecol Obstet Invest 1989; 28: 152-5   DOI   PUBMED   ScienceOn
2 Gurgan T, Urman B, Yarali H, Duran HE. Follicle-stimulating hormone levels on cycle day 3 to predict ovarian response in women undergoing controlled ovarian hyperstimulation for in vitro fertilization using a flare-up protocol. Fertil Steril 1997; 68: 483-7   DOI   ScienceOn
3 Creus M, Penarrubia J, Fabregues F, Vidal E, Carmona F, Casamitjana R, et al. Day 3 serum inhibin B and FSH and age as predictors of assisted reproduction treatment outcome. Hum Reprod 2000; 15: 2341-6   DOI   ScienceOn
4 Muttukrishna S, Suharjono H, McGarrigle H, Sathanandan M. Inhibin B and anti-Mullerian hormone: markers of ovarian response in IVF/ICSI patients? Br J Obstet Gynaecol 2004; 111: 1248-53   DOI   ScienceOn
5 Verberg MF, Eijkemans MJ, Macklon NS, Heijnen EM, Fauser BC, Broekmans FJ. Predictors of low response to mild ovarian stimulation initiated on cycle day 5 for IVF. Hum Reprod 2007; 22: 1919-24   DOI   ScienceOn
6 Klinkert E, te Velde E, Weima S, van Zandvoort P, Hansen R, Nilsson P, et al. FSH receptor genotype is associated with pregnancy but not with ovarian response in IVF. Reprod Biomed Online 2006; 13: 687-95   DOI   PUBMED   ScienceOn
7 Laven JS, Mulders AG, Suryandari DA, Gromoll J, Nieschlag E, Fauser BC, et al. Follicle stimulating hormone receptor polymorphisms in women with normogonadotropic anovulatory infertility. Fertil Steril 2003; 80: 986-92   DOI   ScienceOn
8 Greb RR, Grieshaber K, Gromoll J, Sonntag B, Nieschlag E, Kiesel L, Simoni M. A common single nucleotide polymorphism in exon 10 of the human follicle stimulating hormone receptor is a major determinant of length and hormonal dynamics of the menstrual cycle. J Clin Endocrinol Metab 2005; 90: 4866-72   DOI
9 Davis D, Liu X, Segaloff DL. Identification of the sites of N-linked glycosylation on the follicle-stimulating hormone (FSH) receptor and assessment of their role in FSH receptor function. Mol Endocrinol 1995; 9: 159-70   DOI   ScienceOn
10 윤지성, 최영민, 임경실, 허창영, 가영제, 정재훈 등. 체 외수정 시술시 난포자극호르몬 수용체 유전자 다형성 이 과배란 유도 및 임신 결과에 미치는 영향. 대한불임학회지 2004; 31: 133-9
11 Simoni M, Nieschlag E, Gromoll J. Isoforms and single nucleotide polymorphisms of the FSH receptor gene: implications for human reproduction. Hum Reprod Update 2002; 8: 413 -21   DOI   ScienceOn
12 Liu JY, Gromoll J, Cedars MI. Identification of allelic variants in the follicle-stimulating hormone receptor genes of females with or without hypergonadotropic amenorrhea. Fertil Steril 1998; 70: 326-31   DOI   ScienceOn
13 Devroey P, Bourgain C, Macklon NS, Fauser BC. Reproductive biology and IVF: ovarian stimulation and endometrial receptivity. Trends Endocrinol Metab 2004; 15: 84-90
14 Daelemans C, Smits G, de Maertelaer V, Costagliola S, Englert Y, Vassart G, et al. Prediction of severity of symptoms in iatrogenic ovarian hyperstimulation syndrome by folliclestimulating hormone receptor Ser680Asn polymorphism. J Clin Endocrinol Metab 2004; 89: 6310-5   DOI
15 Kligman I, Rosenwaks Z. Differentiating clinical profiles: prediction good responders, poor responders, and hyperresponders. Fertil Steril 2001; 76: 1185-9   DOI   ScienceOn
16 Nahum R, Shifren JL, Chang Y, Leykin L, Isaacson K, Toth TL. Antral follicle assessment as a tool for predicting outcome in IVF-is it a better predictor than age and FSH? J Assist Reprod Genet 2001; 18: 151-5   DOI   ScienceOn
17 Jun JK, Yoon JS, Ku S-Y, Choi YM, Hwang KR, Park SY, et al. Follicle-stimulating hormone receptor gene polymorphism and ovarian response to controlled ovarian hyperstimulation for IVF-ET. J Hum Genet 2006; 51: 665-70   DOI
18 Fauser BC, Van Heusden AM. Manipulation of human ovarian function: physiological concepts and clinical consequences. Endocr Rev 1997; 18: 71-106   DOI   ScienceOn
19 Barrios-De-Tomasi J, Timossi C, Merchant H, Quintanar A, Avalos JM, Andersen CY, et al. Assessment of the in vitro and in vivo biological activities of the human follicle-stimulating isohormones. Mol Cell Endocrinol 2002; 186: 189-98   DOI   ScienceOn
20 Hendriks DJ, Mol BW, Bancsi LF, Te Velde ER, Broekmans FJ. Antral follicle count in the prediction of poor ovarian response and pregnancy after in vitro fertilization: a metaanalysis and comparison with basal follicle-stimulating hormone level. Fertil Steril 2005; 83: 291-301   DOI   ScienceOn
21 Sudo S, Kudo M, Wada S, Sato O, Hsueh AJ, Fujimoto S. Genetic and functional analyses of polymorphisms in the human FSH receptor gene. Mol Hum Reprod 2002; 8: 893-9   DOI   ScienceOn
22 Ulloa-Aguirre A, Timossi C. Structure-function relationship of follicle-stimulating hormone and its receptor. Hum Reprod Update 1998; 4: 260-83   DOI   ScienceOn
23 Seifer DB, Lambert-Messerlian G, Hogan JW, Gardiner AC, Blazar AS, Berk CA. Day 3 serum inhibin-B is predictive of assisted reproductive technologies outcome. Fertil Steril 1997; 67: 110-4   DOI   ScienceOn
24 Simoni M, Gromoll J, Hoppner W, Kamischke A, Krafft T, Stahle D, et al. Mutational analysis of the follicle-stimulating hormone (FSH) receptor in normal and infertile men: identification and characterization of two discrete FSH receptor isoforms. J Clin Endocrinol Metab 1999; 84: 751-5   DOI
25 Macklon NS, Stouffer RL, Guidice LC, Fauser BC. The science behind 25 years of ovarian stimulation for in vitro fertilization. Endocr Rev 2006; 27: 170-207   DOI   ScienceOn
26 Mayorga MP, Gromoll J, Behre HM, Gassner C, Nieschlag E, Simoni M. Ovarian response to follicle-stimulating hormone stimulation depends on the FSH receptor genotype. J Clin Endocrinol Metab 2000; 85: 3365-9   DOI
27 홍승범, 박동욱, 신미라, 최수진, 이선희, 송인옥 등. 정 산 정자 소견을 나타내는 불임 부부에서 일반적인 체 외수정과 세포질내 정자주입술을 병행하는 분할 수정 법의 효용성. 대한생식의학회지 2007; 34: 305-12
28 Behre HM, Greb RR, Mempel A, Sonntag B, Kiesel L, Kaltwasser P, et al. Significance of a common single nucleotide polymorphism in exon 10 of the follicle-stimulating hormone (FSH) receptor gene for the ovarian response to FSH: a pharmacogenetic approach to controlled ovarian hyperstimulation. Pharmacogenet Genomics 2005; 15: 451-6   DOI
29 De Castro F, Ruiz R, Montoro L, Perez-Hernandez D, Sanchez- Casas Padilla E, Real LM, et al. Role of follicle stimulating hormone receptor Ser680Asn polymorphism in the efficacy of follicle-stimulating hormone. Fertil Steril 2003; 80: 571-6   DOI   ScienceOn