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Gene Expression Profiling Reveals that Paeoniflorin Has an Apoptotic Potential in Human Leukemia U937 Cells  

Lim, Soo-Hyun (Department of Biochemistry, College of Oriental Medicine, Kyung Hee University)
Ahn, Kwang-Seok (Department of Oriental Pathology, College of Oriental Medicine, Kyung Hee University)
Kim, Sung-Hoon (Department of Oriental Pathology, College of Oriental Medicine, Kyung Hee University)
Jang, Hyeung-Jin (Department of Biochemistry, College of Oriental Medicine, Kyung Hee University)
Publication Information
Molecular & Cellular Toxicology / v.5, no.4, 2009 , pp. 335-345 More about this Journal
Abstract
A major source of paeoniflorin (PF) which was from the Paeonia lactiflora root, has been used as a herbal medicine in East Asia for its antiallergic, antiinflammatory, and immunoregulatory effects. However, only few details are known about the mechanism of apoptosis induced by this compound. The present study was undertaken to further elucidate the molecular mechanism of apoptosis and the changes of gene expression elicited by PF using DNA microarrays and computational gene-expression analysis tools in human leukemia U937 cells. A comparative global transcription analysis between treatment with PF and anisomycin (AM) that induces apoptosis in U937 cells revealed that c-Jun-$NH_2$-kinase (JNK) pathway related genes were less expressed in PF-treated cells. Elucidation of the mechanisms by which PF conducts its anti-cancer activities through comparative analysis of the gene expression is necessary to provide a solid foundation for its use as a promising agent in prevention and treatment strategies.
Keywords
Paeoniflorin; Anisomysin; Comparative genomics; U937; Apoptosis;
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