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Heterologous Microarray Hybridization Used for Differential Gene Expression Profiling in Benzo[a]pyrene-exposed Marine Medaka  

Woo, Seon-Ock (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Won, Hyo-Kyoung (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Jeon, Hye-Young (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Kim, Bo-Ra (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Lee, Taek-Kyun (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Park, Hong-Seog (Korea Research Institute of Bioscience and Biotechnology)
Yum, Seung-Shic (South Sea Environment Research Department, Korea Ocean Research and Development Institute)
Publication Information
Molecular & Cellular Toxicology / v.5, no.4, 2009 , pp. 283-290 More about this Journal
Abstract
Differential gene expression profiling was performed in the hepatic tissue of marine medaka fish (Oryzias javanicus) after exposure to benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), by heterologous hybridization using a medaka cDNA microarray. Thirty-eight differentially expressed candidate genes, of which 23 were induced and 15 repressed (P<0.01), were identified and found to be associated with cell cycle, development, endocrine/reproduction, immune, metabolism, nucleic acid/protein binding, signal transduction, or non-categorized. The presumptive physiological changes induced by BaP exposure were identified after considering the biological function of each gene candidate. The results obtained in this study will allow future studies to assess the molecular mechanisms of BaP toxicity and the development of a systems biology approach to the stress biology of organic chemicals.
Keywords
Marine medaka; Oryzias javanicus; Heterologous hybridization; Microarray benzo[a]pyrene (BaP); Differential gene expression profile;
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1 Warshawsky, D. et al. Biotransformation of benzo[a] pyrene and other polycyclic aromatic hydrocarbons and heterocyclic analogs by several green algae and other algal species under gold and white light. Chem Biol Interact 97:131-148 (1995)   DOI   ScienceOn
2 Hawkins, M. B. et al. Identification of a third distinct estrogen receptor and reclassification of estrogen receptors in teleosts. Proc Natl Acad Sci USA 97: 10751-10756 (2000)   DOI   PUBMED   ScienceOn
3 Wahli, W., Martinez, E., Corthesy, B. & Cardinaux, J. R. cis- and trans-acting elements of the estrogen-re- gulated vitellogenin gene B1 of Xenopus laevis. J Steroid Biochem 34:17-32 (1989)   DOI   PUBMED   ScienceOn
4 Reid, K. B. & Porter, R. R. The proteolytic activation systems of complement. Annu Rev Biochem 50:433-464 (1981)   DOI   PUBMED   ScienceOn
5 Barker, C. W., Fagan, J. B. & Pasco, D. S. Down-regulation of P4501A1 and P4501A2 mRNA expression in isolated hepatocytes by oxidative stress. J Biol Chem 269:3985-3990 (1994)   PUBMED   ScienceOn
6 Yaffe, M. B. et al. TCP1 complex is a molecular chaperone in tubulin biogenesis. Nature 358:245-248 (1992)   DOI   PUBMED   ScienceOn
7 Sapieha, P. S. et al. Receptor protein tyrosine phosphatase sigma inhibits axon regrowth in the adult injured CNS. Mol Cell Neurosci 28:625-635 (2005)   DOI   PUBMED   ScienceOn
8 Fons, R. D., Bogert, B. A. & Hegde, R. S. Substratespecific function of the translocon-associated protein complex during translocation across the ER membrane. J Cell Biol 160:529-539 (2003)   DOI   PUBMED   ScienceOn
9 Song, X. et al. A model for the quaternary structure of the proteasome activator PA28. J Biol Chem 271: 26410-26417 (1996)   DOI   PUBMED
10 Moncaut, N., Somoza, G., Power, D. M. & Canario, A. V. Five gonadotrophin-releasing hormone receptors in a teleost fish: isolation, tissue distribution and phylogenetic relationships. J Mol Endocrinol 34: 767- 779 (2005)   DOI   PUBMED   ScienceOn
11 Mussunoor, S. & Murray, G. I. The role of annexins in tumour development and progression. J Pathol 216: 131-140 (2008)   DOI   PUBMED   ScienceOn
12 Liu, S. C. et al. Overexpression of cyclin D2 is assciated with increased in vivo invasiveness of human squamous carcinoma cells. Mol Carcinog 34:131-139 (2002)   DOI   PUBMED   ScienceOn
13 Gracey, A. Y., Troll, J. V. & Somero, G. N. Hypoxiainduced gene expression profiling in the euryoxic fish Gillichthys mirabilis. Proc Natl Acad Sci USA 98: 1993-1998 (2001)   DOI   PUBMED   ScienceOn
14 Buckley, B. A. Comparative environmental genomics in non-model species: using heterologous hybridization to DNA-based microarrays. J Exp Biol 210:1602-1606 (2007)   DOI   PUBMED   ScienceOn
15 Zetterstrom, R. H., Williams, R., Perlmann, T. & Olson, L. Cellular expression of the immediate early transcription factors Nurr1 and NGFI-B suggests a gene regulatory role in several brain regions including the nigrostriatal dopamine system. Brain Res Mol Brain Res 41:111-120 (1996)   DOI   PUBMED   ScienceOn
16 Eltzschig, H. K. et al. HIF-1-dependent repression of equilibrative nucleoside transporter (ENT) in hypoxia. J Exp Med 202:1493-1505 (2005)   DOI   PUBMED   ScienceOn
17 Eddy, S. F. & Storey, K. B. Comparative molecular physiological genomics. Heterologous probing of cDNA arrays. Methods Mol Biol 410:81-110 (2008)   DOI   PUBMED   ScienceOn
18 Quelle, D. E. et al. Overexpression of mouse D-type cyclins accelerates G1 phase in rodent fibroblasts. Genes Dev 7:1559-1571 (1993)   DOI   PUBMED   ScienceOn
19 Imig, J. D. ACE inhibition and bradykinin-mediated renal vascular responses: EDHF involvement. Hypertension 43:533-535 (2004)   DOI   PUBMED   ScienceOn
20 Imai, S., Koyama, J. & Fujii, K. Effects of estrone on full life cycle of Java medaka (Oryzias javanicus), a new marine test fish. Environ Toxicol Chem 26: 726- 731 (2007)   DOI   PUBMED   ScienceOn
21 Schlezinger, J. J. & Stegeman, J. J. Induction and suppression of cytochrome P450 1A by 3,3',4,4',5- pentachlorobiphenyl and its relationship to oxidative stress in the marine fish scup (Stenotomus chrysops). Aquat Toxicol 52:101-115 (2001)   DOI   PUBMED   ScienceOn
22 Chen, J., Shi, Y. H. & Li, M. Y. Changes in transferrin and hepcidin genes expression in the liver of the fish Pseudosciaena crocea following exposure to cadmium. Arch Toxicol 82:525-530 (2008)   DOI   PUBMED   ScienceOn
23 Evans, R. M. The steroid and thyroid hormone receptor superfamily. Science 240:889-895 (1988)   DOI   PUBMED   ScienceOn
24 Yokoe, T. et al. Clinical significance of growth differentiation factor 11 in colorectal cancer. Int J Oncol 31:1097-1101 (2007)   PUBMED   ScienceOn
25 Zupkovitz, G. et al. Negative and positive regulation of gene expression by mouse histone deacetylase 1. Mol Cell Biol 26:7913-7928 (2006)   DOI   PUBMED   ScienceOn
26 Nesto, N. et al. Bioaccumulation and biomarker responses of trace metals and micro-organic pollutants in mussels and fish from the Lagoon of Venice, Italy. Mar Pollut Bull 55:469-484 (2007)   DOI   PUBMED   ScienceOn
27 Dinh, D. T., Frauman, A. G., Johnston, C. I. & Fabiani, M. E. Angiotensin receptors: distribution, signalling and function. Clin Sci (Lond) 100:481-492 (2001)   DOI   PUBMED   ScienceOn
28 Kikuchi, K., Watabe, S. & Aida, K. The Wap65 gene expression of goldfish (Carassius auratus) in association with warm water temperature as well as bacterial lipopolysaccharide (LPS). Fish Physiol Biochem 17:423-432 (1997)   DOI   ScienceOn
29 Griffith, D. A. & Jarvis, S. M. Nucleoside and nucleobase transport systems of mammalian cells. Biochim Biophys Acta 1286:153-181 (1996)   DOI   PUBMED   ScienceOn
30 Wang, Z. et al. RACK1, an excellent predictor for poor clinical outcome in oral squamous carcinoma, similar to Ki67. Eur J Cancer 45:490-496 (2009)   DOI   PUBMED   ScienceOn
31 Morales-Caselles, C., Ramos, J., Riba, I. & Delvalls, T. A. Using the polychaete Arenicola marina to determine toxicity and bioaccumulation of PAHS bound to sediments. Environ Monit Assess 142:219-226 (2008)   DOI   PUBMED   ScienceOn
32 Yu, R. M. et al. Induction of hepatic choriogenin mRNA expression in male marine medaka: a highly sensitive biomarker for environmental estrogens. Aquat Toxicol 77:348-358 (2006)   DOI   PUBMED   ScienceOn
33 Vontas, J. et al. Transcriptional analysis of insecticide resistance in Anopheles stephensi using cross-species microarray hybridization. Insect Mol Biol 16:315-324 (2007)   DOI   PUBMED   ScienceOn
34 Sambrook, J. G., Figueroa, F. & Beck, S. A genomewide survey of Major Histocompatibility Complex (MHC) genes and their paralogues in zebrafish. BMC Genomics 6:152 (2005)   DOI   PUBMED   ScienceOn
35 OECD. in OECD Guideline for Testing of Chemicals (Organisation for Economic Cooperation and Development, Paris, 1992)
36 Bukau, B. & Horwich, A. L. The Hsp70 and Hsp60 chaperone machines. Cell 92:351-366 (1998)   DOI   PUBMED   ScienceOn
37 Woo, S., Yum, S., Kim, D. W. & Park, H. S. Transcripts level responses in a marine medaka (Oryzias javanicus) exposed to organophosphorus pesticide. Comp Biochem Physiol C Toxicol Pharmacol 149: 427-432 (2009)   DOI   PUBMED   ScienceOn
38 Hofbauer, K. H. et al. Oxygen tension regulates the expression of a group of procollagen hydroxylases. Eur J Biochem 270:4515-4522 (2003)   DOI   PUBMED   ScienceOn
39 Cheikyula, J. O., Koyama, J. & Uno, S. Comparative study of bioconcentration and EROD activity induction in the Japanese flounder, red sea bream, and Java medaka exposed to polycyclic aromatic hydrocarbons. Environ Toxicol 23:354-362 (2008)   DOI   PUBMED   ScienceOn
40 Arukwe, A., Yadetie, F., Male, R. & Goksoyr, A. In vivo modulation of nonylphenol-induced zonagenesis and vitellogenesis by the antiestrogen, 3,3'4,4'-tetrachlorobiphenyl (PCB-77) in juvenile fish. Environ Toxicol Pharmacol 10:5-15 (2001)   DOI   PUBMED   ScienceOn
41 Cossins, A. R. & Crawford, D. L. Fish as models for environmental genomics. Nat Rev Genet 6:324-333 (2005)   DOI   PUBMED   ScienceOn
42 Li, J., Mayne, R. & Wu, C. A novel muscle-specific beta 1 integrin binding protein (MIBP) that modulates myogenic differentiation. J Cell Biol 147:1391-1398 (1999)   DOI   PUBMED   ScienceOn
43 Lee, C., Na, J. G., Lee, K. C. & Park, K. Choriogenin mRNA induction in male medaka, Oryzias latipes as a biomarker of endocrine disruption. Aquat Toxicol 61:233-241 (2002)   DOI   PUBMED   ScienceOn
44 Sumpter, J. P. & Jobling, S. Vitellogenesis as a biomarker for estrogenic contamination of the aquatic environment. Environ Health Perspect 103(Suppl 7): 173-178 (1995)   DOI   PUBMED
45 Murata, K. et al. Cloning of cDNA and estrogen-induced hepatic gene expression for choriogenin H, a precursor protein of the fish egg envelope (chorion). Proc Natl Acad Sci USA 94:2050-2055 (1997)   DOI   PUBMED   ScienceOn
46 Hirayama, M., Kobiyama, A., Kinoshita, S. & Watabe, S. The occurrence of two types of hemopexin-like protein in medaka and differences in their affinity to heme. J Exp Biol 207:1387-1398 (2004)   DOI   PUBMED   ScienceOn
47 U.S.EPA. Dose-Response Analysis of Ingested Benzo [a]pyrene (CAS No. 50-32-8) (Human Health Assessment Group, Office of Health and Environmental Assessment, Washington, DC. EPA/600/R-92/045, 1991)
48 Orlando, E. F., Katsu, Y., Miyagawa, S. & Iguchi, T. Cloning and differential expression of estrogen receptor and aromatase genes in the self-fertilizing hermaphrodite and male mangrove rivulus, Kryptolebias marmoratus. J Mol Endocrinol 37:353-365 (2006)   DOI   PUBMED   ScienceOn
49 Castilho, P. C., Buckley, B. A., Somero, G. & Block, B. A. Heterologous hybridization to a complementary DNA microarray reveals the effect of thermal acclimation in the endothermic bluefin tuna (Thunnus orientalis). Mol Ecol 18:2092-2102 (2009)   DOI   PUBMED   ScienceOn
50 Lu, Y. R. et al. A preliminary study on the feasibility of gene expression profile of rhesus monkey detected with human microarray. Transplant Proc 40:598-602 (2008)   DOI   PUBMED   ScienceOn