Browse > Article
http://dx.doi.org/10.5713/ajas.20.0163

Carbonic anhydrase influences asymmetric sodium and acetate transport across omasum of sheep  

Rabbani, Imtiaz (Department of Physiology, University of Veterinary and Animal Sciences)
Rehman, Habib (Department of Physiology, University of Veterinary and Animal Sciences)
Martens, Holger (Institute of Veterinary Physiology, Free University of Berlin)
Majeed, Khalid Abdul (Department of Physiology, University of Veterinary and Animal Sciences)
Yousaf, Muhammad Shahbaz (Department of Physiology, University of Veterinary and Animal Sciences)
Rehman, Zia Ur (Department of Physiology, University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur)
Publication Information
Animal Bioscience / v.34, no.5, 2021 , pp. 880-885 More about this Journal
Abstract
Objective: Omasum is an important site for the absorption of short chain fatty acids. The major route for the transport of acetate is via sodium hydrogen exchanger (NHE). However, a discrepancy in the symmetry of sodium and acetate transport has been previously reported, the mechanism of which is unclear. In this study, we investigated the possible role of carbonic anhydrase (CA) for this asymmetry. Methods: Omasal tissues were isolated from healthy sheep (N = 3) and divided into four groups; pH 7.4 and 6.4 alone and in combination with Ethoxzolamide. Electrophysiological measurements were made using Ussing chamber and the electrical measurements were made using computer controlled voltage clamp apparatus. Effect(s) of CA inhibitor on acetate and sodium transport flux rate of Na22 and 14C-acetate was measured in three different flux time periods. Data were presented as mean±standard deviation and level of significance was ascertained at p≤0.05. Results: Mucosal to serosal flux of Na (JmsNa) was greater than mucosal to serosal flux of acetate (JmsAc) when the pH was decreased from 7.4 to 6.4. However, the addition of CA inhibitor almost completely abolished this discrepancy (JmsNa ≈ JmsAc). Conclusion: The results of the present study suggest that the additional protons required to drive the NHE were provided by the CA enzyme in the isolated omasal epithelium. The findings of this study also suggest that the functions of CA may be exploited for better absorption in omasum.
Keywords
Flux Measurements; Ovine; Short Circuit Current; Ussing Chamber;
Citations & Related Records
연도 인용수 순위
  • Reference
1 von Engelhardt W, Hauffe R. Functions of the omasum in small domestic ruminants. IV. Absorption and secretion of electrolytes. Zentralbl Veterinarmed A 1975;22:367-75. https://doi.org/10.1111/j.1439-0442.1975.tb01442.x   DOI
2 Kramer T, Michelberger T, Gurtler H, Gabel G. Absorption of short-chain fatty acids across ruminal epithelium of sheep. J Comp Physiol B 1996;166:262-9. https://doi.org/10.1007/BF00262870   DOI
3 Ritzhaupt A, Wood IS, Ellis A, Hosie KB, Shirazi-Beechey SP. Identification and characterization of a monocarboxylate transporter (MCT1) in pig and human colon: its potential to transport L-lactate as well as butyrate. J Physiol 1998;513: 719-32. https://doi.org/10.1111/j.1469-7793.1998.719ba.x   DOI
4 Gabel G, Aschenbach JR, Muller F. Transfer of energy substrates across the ruminal epithelium: implications and limitations. Anim Health Res Rev 2002;3:15-30. https://doi.org/10.1079/AHRR200237   DOI
5 Tyagi S, Venugopalakrishnan J, Ramaswamy K, Dudeja PK. Mechanism of n-butyrate uptake in the human proximal colonic basolateral membranes. Am J Physiol Gastrointest Liver Physiol 2002;282:G676-82. https://doi.org/10.1152/ajpgi.00173.2000   DOI
6 von Engelhardt W, Burmester M, Hansen K, Becker G, Rechkemmer G. Effects of amiloride and ouabain on short-chain fatty acid transport in guinea-pig large intestine. J Physiol 1993;460:455-66. https://doi.org/10.1113/jphysiol.1993.sp019481   DOI
7 von Engelhardt W, Burmester M, Hansen K, Becker G. Unidirectional fluxes of short-chain fatty acids across segments of the large intestine in pig, sheep and pony compared with guinea pig. J Comp Physiol B 1995;165:29-36. https://doi.org/10.1007/BF00264683   DOI
8 Smith RH. Microbial activity in the omasum. Proc Nutr Soc 1984;43:63-8. https://doi.org/10.1079/PNS19840028   DOI
9 Tamminga S, van Vuuren AM. Formation and utilization of end products of lignocellulose degradation in ruminants. Anim Feed Sci Technol 1988;21:141-59. https://doi.org/10.1016/0377-8401(88)90096-X   DOI
10 Ali O, Shen Z, Tietjen U, Martens H. Transport of acetate and sodium in sheep omasum: mutual, but asymmetric interactions. J Comp Physiol B 2006;176:477-87. https://doi.org/10.1007/s00360-006-0069-8   DOI
11 Boron WF, Boulpaep EL. Medical physiology: a cellular and molecular approaoch. 3rd ed. Philadelphia, PA, USA: Saunders/Elsevier; 2017.
12 Klisic J, Hu MC, Nief V, et al. Insulin activates Na+/H+ exchanger 3: biphasic response and glucocorticoid dependence. Am J Physiol Renal Physiol 2002;283:F532-9. https://doi.org/10.1152/ajprenal.00365.2001   DOI
13 Spencer AG, Labonte ED, Rosenbaum DP, et al. Intestinal inhibition of the Na+/H+ exchanger 3 prevents cardiorenal damage in rats and inhibits Na+ uptake in humans. Sci Transl Med 2014;6:227ra36. 10.1126/scitranslmed.3007790   DOI
14 Badger MR, Price GD. The role of carbonic anhydrase in photosynthesis. Annu Rev Plant Physiol Plant Mol Biol 1994;45:369-92. https://doi.org/10.1146/annurev.pp.45.060194.002101   DOI
15 Martens H, Kudritzki J, Wolf K, Schweigel M. No evidence for active peptide transport in forestomach epithelia of sheep. J Anim Physiol Anim Nutr 2001;85:314-24. https://doi.org/10.1046/j.1439-0396.2001.00319.x   DOI
16 Allen MS. Relationship between fermentation acid production in the rumen and the requirement for physically effective fiber. J Dairy Sci 1997;80:1447-62. https://doi.org/10.3168/jds.S0022-0302(97)76074-0   DOI
17 Rabbani I, Siegling-Vlitakis C, Noci B, Martens H. Evidence for NHE3-mediated Na transport in sheep and bovine forestomach. Am J Physiol Regul Integr Comp Physiol 2011;301: R313-9. https://doi.org/10.1152/ajpregu.00580.2010   DOI
18 Martens H, Gabel G. Transport of Na and Cl across the epithelium of ruminant forestomachs: rumen and omasum. A review. Comp Biochem Physiol A Physiol 1998;90:569-75. https://doi.org/10.1016/0300-9629(88)90669-X   DOI
19 Caushi D. Transport of HCO3- in sheep omasum [dissertation]. Berlin, Germany: Freie Universitat Berlin; 2015.
20 Gabel G, Sehested J. SCFA transport in the forestomach of ruminants. Comp Biochem Physiol A Physiol 1997;118:36774. https://doi.org/10.1016/S0300-9629(96)00321-0   DOI
21 Charney AN, Micic L, Egnor RW. Nonionic diffusion of short-chain fatty acids across rat colon. Am J Physiol Gastrointest Liver Physiol 1998;274:G518-24. https://doi.org/10.1152/ajpgi.1998.274.3.G518   DOI
22 Petersen KU, Wood JR, Schulze G, Heintze K. Stimulation of gallbladder fluid and electrolyte absorption by butyrate. J Membr Biol 1981;62:183-93. https://doi.org/10.1007/BF01998164   DOI
23 Argenzio RA, Whipp SC. Inter-relationship of sodium, chloride, bicarbonate and acetate transport by the colon of the pig. J Physiol 1979;295:365-81. https://doi.org/10.1113/jphysiol.1979.sp012974   DOI
24 Gabel P, Bestmann M, Martens H. Influences of diet, short-chain fatty acids, lactate and chloride on bicarbonate movement across the reticulo-rumen wall of sheep. J Vet Med A 1991;38:523-9. https://doi.org/10.1111/j.1439-0442.1991.tb01043.x   DOI
25 Muller F, Aschenbach JR, Gabel G. Role of Na+/H+ exchangeand HCO3- transport in pHi recovery from intracellular acid load in cultured epithelial cells of sheep rumen. J Comp Physiol B 2000;170:337-43. https://doi.org/10.1007/s003600000107   DOI
26 Butzner JD, Meddings JB, Dalal V. Inhibition of short-chain fatty acid absorption and Na+ absorption during acute colitis in the rabbit. Gastroenterology 1994;106:1190-8. https://doi.org/10.1016/0016-5085(94)90009-4   DOI
27 Binder HJ, Mehta P. Characterization of butyrate-dependent electroneutral Na-Cl absorption in the rat distal colon. Pflugers Arch 1990;417:365-9. https://doi.org/10.1007/BF00370654   DOI