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http://dx.doi.org/10.9708/jksci.2020.25.06.165

The effect of scopoletin on Aβ-induced neuroinflammatory response in microglial BV-2 cells  

Mun, Hui-Jin (Dept. of Biomedical Laboratory Science, Konyang University)
Cho, Hyun-Jeong (Dept. of Biomedical Laboratory Science, Konyang University)
Publication Information
Journal of the Korea Society of Computer and Information / v.25, no.6, 2020 , pp. 165-170 More about this Journal
Abstract
In this paper, it was confirmed that scopoletin inhibits neuroinflammation induced by amyloid beta oligomer (Aβ1-42) in microglial BV-2. The mechanisms of inflammatory cytokines and inflammatory mediators by scopoletin were identified. Alzheimer's disease is the most common neurodegenerative disease, but it is a disease whose specific etiology is unknown, and many studies are trying to solve it. We first measured the cell viability with the CCK-8 assay method to confirm that scopoletin and Aβ1-42 are toxic to BV-2 cells. Expression levels of interleukin 1 beta (IL-1β), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), and nuclear factor-κB (NF-κB) in inflammatory reactions induced by Aβ1-42 with western blot were analyzed. The ANOVA assay was used to compare protein expression differences between BV-2 cells treated with Aβ1-42 alone and BV-2 cells pretreated with Aβ1-42 and scopoletin. Therefore, this study suggested that scopoletin is worth developing as a neuroinflammatory protection agent for Alzheimer's disease in the future.
Keywords
Alzheimer's disease (AD); Amyloid beta oligomer ($A{\beta_{1-42}$); Scopoletin; Neuroinflammation; Microglial BV-2 cell;
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