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http://dx.doi.org/10.4041/kjod.2009.39.5.337

Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat  

Uysal, Tancan (Department of Orthodontics, Faculty of Dentistry, Erciyes University)
Amasyali, Mihri (Department of Orthodontics, Centre of Dental Sciences, Gulhane Military Medical Academy)
Olmez, Huseyin (Department of Orthodontics, Centre of Dental Sciences, Gulhane Military Medical Academy)
Karslioglu, Yildirim (Department of Pathology, Faculty of Medicine, Gulhane Military Medical Academy)
Gunhan, Omer (Department of Pathology, Faculty of Medicine, Gulhane Military Medical Academy)
Publication Information
The korean journal of orthodontics / v.39, no.5, 2009 , pp. 337-347 More about this Journal
Abstract
Objective: The aim of this study was to evaluate the effects of vitamin E ($\alpha$-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. Methods: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of $\alpha$-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg $\alpha$-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p < 0.05 level. Results: New bone area, bone perimeter, feret's diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p < 0.001). Bone architecture in $\alpha$-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. Conclusions: The application of $\alpha$-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.
Keywords
Vitamin E; $\alpha$-tocopherol; Expansion; Histomorphometry; Rat;
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