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http://dx.doi.org/10.15204/jkobgy.2014.27.4.001

Antimetastatic Effects of Jipae-san by Inflammation Control and Activation of Innate Immune System  

Heo, Su-Jeong (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Hwang, Deok-Sang (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Jin-Moo (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Chang-Hoon (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Lee, Kyung-Sub (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Jang, Jun-Bock (Dept. of Gynecology, College of Korean Medicine, Kyung-Hee University)
Publication Information
The Journal of Korean Obstetrics and Gynecology / v.27, no.4, 2014 , pp. 1-14 More about this Journal
Abstract
Objectives: This study was designed to investigate the anti-tumor metastasis by anti-inflammatory and innate immunomodulating effects of extracts of Jipae-san on cancer cells. Methods: Antimetastatic experiments were conducted in vivo mouse model by using 4T1 mouse mammary carcinoma cells. Cell viability of Jipae-san was tested with 4T1 mouse mammary carcinoma cells, colon 26-M3.1 carcinoma cells and macrophage. In addition expression of $TNF-{\alpha}$ and NO induced by LPS was measured after treating with Jipae-san. To observe innate immunomodulating effects of Jipae-san on macrophage, we measured $TNF-{\alpha}$, IL-12, IL-6 and MCP-1, respectively. Cell cytotoxicity was tested with the macrophage stimulated with Jipae-san and we evaluated the activation of $TNF-{\alpha}$ and NO. And the effect of Jipae-san on metastasis was measured without NK-cell using GM1 serum. Results: Intravenous inoculation of Jipae-san significantly inhibited metastasis of 4T1 mouse mammary carcinoma cells. In an in vitro cytotoxicity analysis, cell growth are closer to 100% less than $1,000{\mu}g/ml$ concentration. The expression of $TNF-{\alpha}$ and NO induced by LPS after treating Jipae-san was down regulated in dose-dependent manner. Level of cytokines such as $TNF-{\alpha}$, IL-12, IL-6 and MCP-1 of Jipae-san group were up regulated in compared to the control group. The macrophage stimulated with Jipae-san significantly inhibits the cancer cell at ratio of 10:1, 20:1. The activation of NO was significantly up regualted in a group of 5:1, 10:1, 20:1. The depletion of NK-cells by anti-asialo GM1 serum partly abolished the inhibitory effect of Jipae-san on tumor metastasis. Conclusions: Jipae-san appears to have considerable activity on the anti-metastasis by inflammation control and activation of innate immune system.
Keywords
Jipae-san; Cancer; Inflammation control; Innate immune system; Anti-metastasis; Complementary therapies;
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Times Cited By KSCI : 8  (Citation Analysis)
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