Effects of 1% dietary orotic acid on triglyceride metabolism were examined in SD-rats and Kud: ddY mice. When rats were fed semisynthetic diet containing 1% orotic acid and n-6 polyunsaturated fatty acid (linoleic acid), the hepared diet. In contrast to rats which respond to orotic acid consumption with increases in hepatic triglyceride content, mice did not so respond. The rats-limiting step in triglyceride synthesis is catalyzed by the enzyme phosphatic acid phosphohydrolase (EC3.1.3.4) which is present in the liver cytosol and microsomes of rats fed oroic acid diet. This finding suggests that the activity of this enzyme may play a tole in the fatty liver formation in rats.

The action of phospholipid on the rhodotorucine A(RH.A) acceptance by heterbasidiomyceteous yeast Rhodosporidium toruloides mating type a cells and the effect of medium composition during sexual differentiation were investigated. Activation of trigger peptidase(TPase)was very sensitive to the originated phospholipid from R. toruloides and was more sensitive to phospholipid liposome made up of phospholipi. Phospholopod present on the membrance of mating type a cells consists of phospatidylglycerol(PG), phosphatidylethanolamine(PE), phospatidylcholine(PC), phospatidylinositol(PI), and phosphatidylserine(PS) of 12.9, suprisingly 45.4, 11.0, and 13.9%, respectively. As the result of using C-1 and N-1 mediums which limited C and N sources capable of inhibiting the synthesis of phospholipid, it resulted inhibiting sexual dlfferentiation and production of Rh.A from mating type Acells.

It was carried out to find the carbohydrate metabolism for the glycogen utilization and the effects of low temperature(1$\circ$C, 5$\circ$C)on embryonic development of the silkworm, Bombyx mori L. The content of sorbitol and glycerol in the chillihg eggs stored at 5$\circ$C or 1$\circ$C on 24 hours after oviposition showed the highest level on the 50th day. After that, the corresponding value in the eggs stored at 5$\circ$C were decreased, and the value in the eggs stored at 1$\circ$C maintained the similar level. The trehalose level was rapidly increased on 50$\sim$60th day or thereabouts, and then, it was accumulated. When the eggs stored at low temperature were tranaferred and developed the embryo at 25$\circ$C, sorbitol and glycerol were respectively changed to glycogen. Futhemore, the content of trehalose and glucose showed specific of patterns with each embryonic stage at low temperature. With the aforementioned results, the intemediary metabolism of carbohydrate was discussed with regard to embryonic development of non-diapause eggs of silkworms.

Aloe(Aloe vera LINNE) has been used as a home medicine for the past several thousand in the world, and has been studied on various chronic degenerative diseases such as atherosclerosis, myocardiac infarction and hypertension. SMAP8, learning and memory impairment animal mode, were fed basic or experimental diets with 1.0% of freeze dried(FD)-Aloe powder for 8 months. This study was designed to investigate the effects of Aloe on body weight gain, grading score of senescence(GSS), triglyceride, total and LDL-cholesterol levels, and atherogenic index in serum of SAMP8, and also designed to investigate the effects of Aloe on cholesterol accumultions in mitochondria and microsome fractions of SAMP8 brain. Body weight gain was consistently lower in aloe group than in control group, but no significantly differences between them. Grading score of senescence resulted ina marked decreases pf 20% in 1.0% Aloe group compared with control group. Administrations of 1.0% aloe resulted ina marked decreases in 15% and 20% of triglyceride and cholesterol levels, respectively, and also significantly decreased in 15% of LDL-cholesterol levels and atherogenic index in serum of SAMP8 compared with control group. Cholesterol accumulations were significantly inhibited in 20% and 10% of mitochondria and microsome fractions of SAMP8 brain, respectively, by administration of 1.0% Aloe. These results suggest that administration of Aloe mau not only effectively inhibit chronic degenerative diseases in serum of SAMP8, but may also improve learning and memory impairments of SAMP8 brain.

박테리오파지 T7 gene 2.5 단백질은 single-stranded DNA 결합 단백질로 박태리오파지 T7의 DNA복제, 재조합, 및 수선에 필수적으로 요구된다. Gene 2.5 protein은 T7의 DNA 합성과 성장에 필수적인 단백질이다. Gene 2.5 Protein이 중요시 되는 이유는 이 단백질이 T7의 다른 복제 필수단백질인 T7의 다른 복제 필수단백질인 T7 DNA polymerase 와 gene 4 protein(helicase/primase)와 서로 상호작용할 것으로 제안되었기 때문이다. (Kim and Richardson, J. Biol. Chem., 1992;1994). 이 단백질의 단백질 상호작용을 가능하게 하는 domain은 carboxyl-terminal domain일 것으로 여러 실험에서 대두되었기에, 이 domain의 특성을 파악하기 위해 야생형과 변이체 gene 2.5 단백질들을 각각 GST에 융합한후 fusion 단백질을 정제하였다. 정제된 이 융합 단백질들의 carboxyl-terminal domain이 T7 복제 단백질들과 상호작용을 조사하는지를 조사하기 위해 affinity chromatography로 이용하였다. 실험 결과, 아생형 GST-gene 2.5 융합단잭질(GST-2.5 (WT))는 T7 DNA polymerase 와 상호작용을 하였지만. 변이형 융합단백질(GST-2.5$\Delta$21C)는 interaction을 하지 못했다. 이 결과는 carbohyl-terminal domain이 단백질-단백질 상호작용을 하는데 직접적으로 관여하는 것을 증명하였다. 또한,GST2.5(WT)는 gene 4 protein(helicase/primase)와 직접 상호작용을 하나. GST2.5$\Delta$21C는 상호작용을 하지 못하는 것으로 나타났다. 따라서 gene 4 proteins와의 상호작용에도 gene 2.5 protein의 carboxyl-terminal domain이 직접 관여 한다는 것이 증명되었다. 이상의 결과에서 gene 2.5 protein은 박테리오파지 T7 의 유전자 목제 시 단백질-단백질 상호작용에 관혀아며, 특히 gene 2.5 protein의 carboxyl-terminal domain이 이러한 상호작용에 직접적으로 관여하는 domain이라는 것을 알 수가 있었다.

The action spectrum for mycelial growth and fruitbody primordium formation in Pleurotus ostreatus has been studied by irradiation at various wavelengths. Effective wavelengths. Effective wavelengths were distributed from near ultraviolet to blue region of spectrum. The most effect of light was observed in the region between 340 to 500 nanometers. The light intensity required to obtain of the maximum effect at the most effective wavelengths(430-500nm), was over 6.8 mW/Cm$^{2}$. Up to 6.8 mW/Cm$^{2}$, the primordium formed about 25 hr after the start of illumination. The higher the light intensity, the earlier the formation of the primordium formation : Up to about 6.8 mW/Cm$^{2}$.

A thermolabile alkaline phosphatase has been purified through steps of osmotic shock, ammonium sulfate salting-out, and DAEA-cellulose chromatography from the cultured broth of the marine Vibrio sp. M-96 strain. The optimal temperature for the enzyme activity was 35$\circ$C. The optimal pH was pH11.0, and the range of pHstability was pH10.4 to 12.0. Thermal inactivation occured within 6 mintes at 60$\circ$C. The enzyme was considerably inactivated by 0.1mM concentrations of Hg$^{2+}$, Ni$^{2+}$ and Zn$^{2+}$, whereas activated up to 234% by 1mM of Mn$^{2+}$. The activation energy and deactivation energy by the Arrhenius equation were 4.02 Kcal/mol and 9.098 Kcal/mol, respectively. The Km and Vmax values of the enzyme for p-introphenylphosphate were found to be 0.0465mM and 0.001334mM/min, respectively. Active form of the enzyme had a molecular weight of 57,000 dalton determined by the Sephadex G-200 gel filtration method.

본 연구는 포유동물 수정란의 체외발생에 인, 아미노산과 BSA가 미치는 영향을 조사하기 위하여 체외성숙 및 수정된 소의 수정란을 한정 무단백 배양액에서 배양하였다. 배양액에 포함된 인의 농도를 0.00, 0.10. 0.35, 1.05와 2.10mM로 조정하였을 때 0.00부터 1.05mM 농도에서는 수정후 48시간 까지 수정란 발생에 영향을 미치지 않았고, 수정후 96시간과 114에서의 8세포기와 상실배 발생이 0.35mM에서 유의적(P<0.05)으로 붕가하였다. 19종 아미노산의 첨가는 수정후 96, 144, 192시간에 각각8세포기(49-50%), 상실배(38-40%), 배반포(29-32%)발생을 유의적(P<0.05)으로 증가하였으나, glutamine단독 첨가는 영향이 없었다. BSA첨가는 첨가시간에 관계없이 수정후 48, 96, 144, 192시간에서 각각 2세포기, 8세포기, 상실배와 배반포의 발생을 증가시켰다.

대장균의 GroEL과 상동성을 가지는 symbionin이 진딧물의 세포내 곤생미생물에서 유일하게 생산된다. 이것은 in vitro와 in vivo에서 moecular chaperone 활성을 가지는 것과 함께 자가인산화(autophosphory-lation)와 인산기전이효소(phosphotransferase)의 활성에 의해서 고에너지 인산기를 다른 곳에 줄 수 있다. Symbionin은 two component pathway의 센서분자의 역할을 하며, 지금까지 알려진 것과는 다른 성질을 가진 protein Kinase이다.