Animal Bioscience

  • 제39권7호
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  • Pages.250538.1-250538.15
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  • 2026
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Functional characterization of porcine septin12 and its role in male reproduction

  • Pei Wang (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Xia Zhang (Department of Biological and Food Engineering, Lyuliang University) ;
  • Hailong Huo (Yunnan Open University) ;
  • Luogang Li (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Feidi Wen (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Shuyan Wang (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • YongYun Zhang (Teaching Demonstration Center of the Basic Experiments of Agricultural Majors, Yunnan Agricultural University) ;
  • Renaguli Turgong (College of Animal Science and Technology, Yunnan Agricultural University) ;
  • Jinlong Huo (College of Animal Science and Technology, Yunnan Agricultural University)
  • 투고 : 2025.07.25
  • 심사 : 2026.03.29
  • 발행 : 2026.07.01
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초록

Objective: The objective is to identify and functionally characterize the pig septin12 gene, focusing on its expression pattern, subcellular localization, and interacting proteins, in order to explore its potential roles in male reproduction. Methods: Full-length septin12 cDNA was obtained from the testis of the Banna mini-pig inbred line (BMI) using rapid amplification of cDNA ends. Quantitative real-time polymerase chain reaction and western blot were employed to assess tissue-specific expression in BMI pigs. Subcellular localization was determined by fluorescence microscopy following transfection of EGFP-tagged septin12. A yeast two-hybrid (Y2H) screen using an adult BMI testis cDNA library identified potential interacting proteins, which were then validated by Co-immunoprecipitation (Co-IP) and immunofluorescence (IF). Gene Ontology (GO) analysis was performed to ascertain the functional categories of the interactors. Results: The cloned septin12 cDNA from the BMI testis was 1,289 base pairs (bp) in length. Septin12 mRNA and protein expression was predominantly observed in the BMI testis, suggesting its critical role in male reproductive function. Septin12 localized to both fibrous filamentous and punctate cytoplasmic structures. Thirteen interacting proteins were identified by Y2H, including ATAD5, ATP1B3, CENPL, ENO1, and septin5, with GO analysis indicating enrichment in mitotic nuclear division, cell growth, and G2/M phase transition. Furthermore, Co-IP and IF confirmed interactions between septin12 and electron transfer flavoprotein subunit alpha (ETFA) as well as lactate dehydrogenase B (LDHB), supporting the results of the Y2H screen. Conclusion: This study identified and characterized the septin12 gene in BMI pig, highlighting its predominant expression in the testis and implicating its role in male reproductive function. Protein interaction analysis uncovered partners involved in cell division and metabolism, suggesting potential mechanisms by which septin12 may influence spermatogenesis. Co-IP and IF further validated interactions with ETFA and LDHB. These findings offer a groundwork for future investigations on the role of septin12 in male fertility.

키워드

과제정보

This research was funded by the National Natural Science Foundation of China (Nos. 31460580 and 32060733 to J.L.H; No. 31660637 to P.W.); Agricultural Joint Project of the Yunnan Province Science and Technology Department (No. 202401BD070001-004 to J.L.H.); Basic Research Key Project of Yunnan Province, China (No. 202501AS070041 to J.L.H.); Yunnan Provincial Department of Education Scientific Research Fund Project, China (No. 2024J0451 to P.W.); College Student Innovation and Entrepreneurship Training Program (No. S202210676062 to R.N.G.L.T.R.G.).

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