Animal Bioscience

  • 제39권7호
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  • Pages.250935.1-250935.14
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  • 2026
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Resveratrol inhibits NLRP3 inflammasome activation to alleviate bovine mastitis by promoting PINK1-mediated mitophagy

  • Junpeng Huang (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Longwei Sun (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Shujing Tan (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Ran Yu (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Weiguo Zhao (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology) ;
  • Chengmin Li (Jiangsu Key Laboratory of Sericultural and Animal Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology)
  • 투고 : 2025.12.01
  • 심사 : 2026.03.02
  • 발행 : 2026.07.01
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초록

Objective: Negative energy balance in transition cows elevates the circulating concentrations of non-esterified fatty acids (NEFA), potentially leading to mastitis and posing a significant threat to the dairy industry. Resveratrol is a polyphenolic compound with anti-inflammatory properties, yet its role in NEFA-induced inflammation in bovine mammary epithelial cells (BMECs) remains unclear. This study aimed how resveratrol protects against mastitis and its underlying mechanisms. Methods: BMECs were pre-treated with 100 µM resveratrol for 24 h, and then treated with 0.9 mM NEFAs for 4 h, and a PINK1 inhibitor was used to assess the mechanisms involved. Furthermore, a mouse model of mastitis was utilized to evaluate the hepatoprotective effects of resveratrol against mastitis in vivo. Results: Resveratrol significantly attenuated the NEFA-induced inflammatory response, evidenced by reduced levels of NLRP3 inflammasome components (NLRP3, caspase1, IL-1β) and pro-inflammatory cytokines (IL-6, IL-1β and TNF-α). Mechanistically, resveratrol promoted mitophagy by upregulating levels of LC3-II, PINK1, and Parkin, while downregulating P62 expression. The anti-inflammatory effect of resveratrol was reversed when PINK1 was inhibited. In vivo experiments confirmed that resveratrol alleviated mammary gland inflammation and enhanced PINK1-mediated mitophagy. Conclusion: This study demonstrates that resveratrol mitigates NLRP3-mediated inflammation by activating PINK1-mediated mitophagy, suggesting its potential as a therapeutic option for mastitis in perinatal dairy cows with negative energy balance.

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과제정보

This research was supported by the National Natural Science Foundation of China (Grant No. 32002161), the Ministry of Science and Technology of China (KY202201002), the earmarked Fund of CARS-18, Postgraduate Research & Practice Innovation Program of Jiangsu Province (KYCX2 2_3866).

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