Animal Bioscience

  • 제38권8호
  • /
  • Pages.1798-1816
  • /
  • 2025
  • /
  • 2765-0189(pISSN)
  • /
  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Molecular regulatory mechanisms of dietary supplementation with Allium mongolicum Regel powder to improve muscle development and meat quality in Angus calves

  • Wangjing Liu (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Chenxu Sun (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Huixia Gao (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Jianjian He (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Aihuan Yu (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Yaodi Xie (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Haibo Yao (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Jiang Hu (College of Animal Science and Technology, Gansu Agricultural University) ;
  • Zhaomin Lei (College of Animal Science and Technology, Gansu Agricultural University)
  • 투고 : 2024.11.18
  • 심사 : 2025.01.17
  • 발행 : 2025.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Objective: Supplementing animal feed with Allium mongolicum Regel powder (AMRP) additives can promote muscle production and improve meat quality. Here, we explored the effects of dietary AMRP supplementation on the performance, meat quality, and muscle transcriptome profile of Angus calves. Methods: Twelve healthy female black Angus calves (average body weight = 280.4±15.74 kg, average age = 14±0.6 months) of the same genetic background were randomly assigned to two feed groups: control (CON; basal diet without any supplementation) and AMRP (basal diet supplemented with 20 g of AMRP per calf per day). Results: In general, compared with the control group, dietary AMRP supplementation significantly increased the longissimus thoracis muscle area (p = 0.027) and pH24h (p = 0.027) but significantly reduced Warner-Bratzler shear force (p = 0.009) and cooking loss (p<0.001). Moreover, 1,284 differentially expressed genes (DEGs) were identified in AMRP-supplemented Angus calves. Pathway analysis revealed that the DEGs were involved in multiple pathways related to muscle development and fat deposition, such as the focal adhesion and MAPK pathways. Conclusion: Dietary supplementation of AMRP improved muscle growth and development in Angus beef cattle. It also significantly modulated meat quality, possibly altering signaling pathways by influencing key gene expression. Our results provide novel insights into the development of the meat industry and indicate the mechanism through which AMRP regulates muscle development and improves meat quality at the molecular level.

키워드

과제정보

The authors would like to thank Aoxin (Tianjin) Farming Co., Ltd., for granting access to the experimental site.

참고문헌

  1. Zheng Z, Henneberry SR, Zhao Y, Gao Y. Predicting the changes in the structure of food demand in China. Agribusiness 2019;35:301-28. https://doi.org/10.1002/agr.21592
  2. Zhu X, Ren L, Liu J, et al. Transcriptome analysis provides novel insights into the function of PI3K/AKT pathway in maintaining metabolic homeostasis of Chinese perch muscle. Aquacult Rep 2021;21:100838. https://doi.org/10.1016/j.aqrep.2021.100838
  3. Gomes RC, Silva SL, Carvalho ME, et al. Protein synthesis and degradation gene SNPs related to feed intake, feed efficiency, growth, and ultrasound carcass traits in Nellore cattle. Genet Mol Res 2013;12:2923-36. https://doi.org/10.4238/2013.August.12.8
  4. Du L, Li K, Chang T, et al. Integrating genomics and transcriptomics to identify candidate genes for subcutaneous fat deposition in beef cattle. Genomics 2022;114:110406. https://doi.org/10.1016/j.ygeno.2022.110406
  5. Ramalingam V, Hwang I. Identification of meat quality determining marker genes in fibroblasts of bovine muscle using transcriptomic profiling. J Agric Food Chem 2021;69:3776-86. https://doi.org/10.1021/acs.jafc.0c06973
  6. He P, Lei Y, Zhang K, et al. Dietary oregano essential oil supplementation alters meat quality, oxidative stability, and fatty acid profiles of beef cattle. Meat Sci 2023;205:109317. https://doi.org/10.1016/j.meatsci.2023.109317
  7. Liu W, Ao C. Effect of dietary supplementation with Allium mongolicum Regel extracts on growth performance, carcass characteristics, and the fat color and flavor-related branched-chain fatty acids concentration in ram lambs. Anim Biosci 2021;34:1134-45. https://doi.org/10.5713/ajas.20.0246
  8. Liu W, Tang D, Ao C. Adding of Allium mongolicum regel extracts to lamb feedlot diets influences 4-alkyl-branched fatty acids deposition and the meat quality during storage. Meat Sci 2022;193:108951. https://doi.org/10.1016/j.meatsci.2022.108951
  9. Liu W, Yu A, Xie Y, et al. Drying enhances the antioxidant activity of Allium mongolicum Regel through the phenylpropane and AA-MA pathway as shown by metabolomics. Food Chem X 2024;22:101436. https://doi.org/10.1016/j.fochx.2024.101436
  10. Liu W, Gao H, He J, et al. Effects of dietary Allium mongolicum Regel powder supplementation on the growth performance, meat quality, antioxidant capacity and muscle fibre characteristics of fattening Angus calves under heat stress conditions. Food Chem 2024;453:139539. https://doi.org/10.1016/j.foodchem.2024.139539
  11. Honikel KO. Reference methods for the assessment of physical characteristics of meat. Meat Sci 1998;49:447-57. https://doi.org/10.1016/S0309-1740(98)00034-5
  12. Kim D, Langmead B, Salzberg SL. HISAT: a fast spliced aligner with low memory requirements. Nat Methods 2015;12:357-60. https://doi.org/10.1038/nmeth.3317
  13. Pertea M, Pertea GM, Antonescu CM, Chang TC, Mendell JT, Salzberg SL. StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol 2015;33:290-5. https://doi.org/10.1038/nbt.3122
  14. Li B, Dewey CN. RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome. BMC Bioinformatics 2011;12:323. https://doi.org/10.1186/1471-2105-12-323
  15. Zhao S, Li CI, Guo Y, Sheng Q, Shyr Y. RnaSeqSampleSize: real data based sample size estimation for RNA sequencing. BMC Bioinformatics 2018;19:191. https://doi.org/10.1186/s12859-018-2191-5
  16. Purslow PP, Warner RD, Clarke FM, Hughes JM. Variations in meat colour due to factors other than myoglobin chemistry; a synthesis of recent findings (invited review). Meat Sci 2020;159:107941. https://doi.org/10.1016/j.meatsci.2019.107941
  17. Kirkpatrick LT, Gómez JFM, Beline M, et al. Muscle of dark and normal beef differs metabolically. Meat Sci 2023;206:109344. https://doi.org/10.1016/j.meatsci.2023.109344
  18. Harris CL, Wang B, Deavila JM, et al. Vitamin A administration at birth promotes calf growth and intramuscular fat development in Angus beef cattle. J Anim Sci Biotechnol 2018;9:55. https://doi.org/10.1186/s40104-018-0268-7
  19. Talon S, Giroux-Metges MA, Pennec JP, Guillet C, Gascan H, Gioux M. Rapid protein kinase C-dependent reduction of rat skeletal muscle voltage-gated sodium channels by ciliary neurotrophic factor. J Physiol 2005;565:827-41. https://doi.org/10.1113/jphysiol.2005.084681
  20. Castro BM, Prieto M, Silva LC. Ceramide: a simple sphingolipid with unique biophysical properties. Prog Lipid Res 2014;54:53-67. https://doi.org/10.1016/j.plipres.2014.01.004
  21. de Oliveira Monteschio J, de Souza KA, Vital ACP, et al. Clove and rosemary essential oils and encapsuled active principles (eugenol, thymol and vanillin blend) on meat quality of feedlot-finished heifers. Meat Sci 2017;130:50-7. https://doi.org/10.1016/j.meatsci.2017.04.002
  22. Bonnet M, Cassar-Malek I, Chilliard Y, Picard B. Ontogenesis of muscle and adipose tissues and their interactions in ruminants and other species. Animal 2010;4:1093-109. https://doi.org/10.1017/S1751731110000601
  23. Dietze D, Koenen M, Röhrig K, Horikoshi H, Hauner H, Eckel J. Impairment of insulin signaling in human skeletal muscle cells by co-culture with human adipocytes. Diabetes 2002;51:2369-76. https://doi.org/10.2337/diabetes.51.8.2369
  24. Guo L, Cui H, Zhao G, et al. Intramuscular preadipocytes impede differentiation and promote lipid deposition of muscle satellite cells in chickens. BMC Genomics 2018;19:838. https://doi.org/10.1186/s12864-018-5209-5
  25. Lee EJ, Lee HJ, Kamli MR, et al. Depot-specific gene expression profiles during differentiation and transdifferentiation of bovine muscle satellite cells, and differentiation of preadipocytes. Genomics 2012;100:195-202. https://doi.org/10.1016/j.ygeno.2012.06.005
  26. Lim IR, Kim C, Jung JW, Kim JH, Hong SJ. Inhibition of smooth muscle cell proliferation and migration by a Talin modulator attenuates neointimal formation after femoral arterial injury. Korean Circ J 2020;50:613-24. https://doi.org/ 10.4070/kcj.2019.0421.
  27. Di Caprio N, Bellas E. Collagen stiffness and architecture regulate fibrotic gene expression in engineered adipose tissue. Adv Biosyst 2020;4:1900286. https://doi.org/10.1002/adbi.201900286
  28. Greenberg AS, Shen WJ, Muliro K, et al. Stimulation of lipolysis and hormone-sensitive lipase via the extracellular signal-regulated kinase pathway. J Biol Chem 2001;276:45456-61. https://doi.org/10.1074/jbc.m104436200
  29. Yee KL, Weaver VM, Hammer DA. Integrin-mediated signalling through the MAP-kinase pathway. IET Syst Biol 2008;2:8-15. https://doi.org/10.1049/iet-syb:20060058
  30. Li B, Qiao L, An L, et al. Transcriptome analysis of adipose tissues from two fat-tailed sheep breeds reveals key genes involved in fat deposition. BMC Genomics 2018;19:338. https://doi.org/10.1186/s12864-018-4747-1
  31. Janin A, Bessière F, Chauveau S, Chevalier P, Millat G. First identification of homozygous truncating CSRP3 variants in two unrelated cases with hypertrophic cardiomyopathy. Gene 2018;676:110-6. https://doi.org/10.1016/j.gene.2018.07.036
  32. Choi YO, Ryu HJ, Kim HR, et al. Implication of phosphorylation of the myosin II regulatory light chain in insulin-stimulated GLUT4 translocation in 3T3-F442A adipocytes. Exp Mol Med 2006;38:180-9. https://doi.org/10.1038/emm.2006.22
  33. Xue J, Lv Q, Khas E, et al. Tissue-specific regulatory mechanism of LncRNAs and methylation in sheep adipose and muscle induced by Allium mongolicum Regel extracts. Sci Rep 2021;11:9186. https://doi.org/10.1038/s41598-021-88444-9
  34. Meister M, Tomasovic A, Banning A, Tikkanen R. Mitogen-activated protein (MAP) kinase scaffolding proteins: a recount. Int J Mol Sci 2013;14:4854-84. https://doi.org/10.3390/ijms14034854
  35. Hartl FU, Hayer-Hartl M. Molecular chaperones in the cytosol: from nascent chain to folded protein. Science 2002;295:1852-8. https://doi.org/10.1126/science.1068408
  36. Ding Z, Huang F, Zhang C, Zhang L, Sun H, Zhang H. Effect of heat shock protein 27 on the in vitro degradation of myofibrils by caspase-3 and μ-calpain. Int J Food Sci Technol 2018;53:121-8. https://doi.org/10.1111/ijfs.13565
  37. Contreras-Castillo CJ, Lomiwes D, Wu G, Frost D, Farouk MM. The effect of electrical stimulation on post mortem myofibrillar protein degradation and small heat shock protein kinetics in bull beef. Meat Sci 2016;113:65-72. https://doi.org/10.1016/j.meatsci.2015.11.012
  38. Riley LA, Zhang X, Douglas CM, et al. The skeletal muscle circadian clock regulates titin splicing through RBM20. eLife 2022;11:e76478. https://doi.org/10.7554/eLife.76478
  39. Robinson I, Reddy AB. Molecular mechanisms of the circadian clockwork in mammals. FEBS Lett 2014;588:2477-83. https://doi.org/10.1016/j.febslet.2014.06.005
  40. Luo B, Zhou X, Tang Q, et al. Circadian rhythms affect bone reconstruction by regulating bone energy metabolism. J Transl Med 2021;19:410. https://doi.org/10.1186/s12967-021-03068-x
  41. Zhang X, Patel SP, McCarthy JJ, Rabchevsky AG, Goldhamer DJ, Esser KA. A non-canonical E-box within the MyoD core enhancer is necessary for circadian expression in skeletal muscle. Nucleic Acids Res 2012;40:3419-30. https://doi.org/10.1093/nar/gkr1297