Animal Bioscience

  • 제38권8호
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  • Pages.1570-1579
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  • 2025
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Analysis of DNA methylation changes following Cfp1 knockout in mouse spermatocytes

  • Chanhyeok Park (Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University) ;
  • Youngsok Choi (Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University) ;
  • Seonho Yoo (Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University) ;
  • Hyeonwoo La (Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University) ;
  • Kwonho Hong (Department of Stem Cell and Regenerative Biotechnology, Institute of Advanced Regenerative Science, Konkuk University)
  • 투고 : 2024.11.18
  • 심사 : 2025.01.10
  • 발행 : 2025.08.01
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초록

Objective: Spermatogenesis is a complex biological process that encompasses meiosis in spermatocytes and the dynamic epigenetic alterations that ensure the inheritance of genetic traits. CXXC finger protein 1 (CFP1, Cfp1, CXXC1, Cxxc1) is a critical component of the SET domain-containing 1A histone lysine methyltransferase complex that catalyzes histone H3K4 methylation and has a specific binding domain for unmethylated CpG DNA. However, our current understanding of CFP1’s role in the genome-wide regulation of DNA and H3K4 methylation remains limited. Methods: We performed genome-wide methylation analysis using reduced-representation bisulfite sequencing on spermatocytes isolated from Cfp1 knockout and wild-type mice. Promoter methylation changes were integrated with publicly available microarray and ChIP-seq data to identify genes regulated by CFP1. Results: CFP1 depletion led to significant alterations in DNA methylation, particularly in promoter regions of genes associated with meiosis, transcription regulation, and chromatin remodeling. A total of 21 genes were identified as direct targets of CFP1, exhibiting reduced promoter methylation and CFP1 binding. Conclusion: Our study findings contribute to elucidating the regulatory mechanisms of CFP1 in spermatocytes, providing valuable insights into the reproductive process and advancing our understanding of the underlying causes of infertility.

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과제정보

This research was supported by the Ministry of Science and ICT (RS-2024-00344340) of the Korean government.

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