Animal Bioscience

  • 제38권2호
  • /
  • Pages.267-277
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  • 2025
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  • 2765-0189(pISSN)
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  • 2765-0235(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Porcine granulosa cell transcriptomic analyses reveal the differential regulation of lncRNAs and mRNAs in response to all-trans retinoic acid in vitro

  • Jinzhu Meng (College of Veterinary Medicine, Hunan Agricultural University) ;
  • Xiuwen Chen (College of Veterinary Medicine, Hunan Agricultural University) ;
  • Huabiao Wang (College of Veterinary Medicine, Hunan Agricultural University) ;
  • Yixuan Mi (College of Veterinary Medicine, Hunan Agricultural University) ;
  • Runsheng Zhou (College of Veterinary Medicine, Hunan Agricultural University) ;
  • Hongliang Zhang (College of Veterinary Medicine, Hunan Agricultural University)
  • 투고 : 2024.05.27
  • 심사 : 2024.08.12
  • 발행 : 2025.02.01
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초록

Objective: The active metabolite of vitamin A, all-trans retinoic acid (ATRA), is involved in the proliferation and differentiation of granulosa cells, and promotes the follicular development, oocyte maturation, and ovulation in mammals. This study aims to investigate the ATRA induced potential long noncoding RNAs (lncRNAs) that regulate the expression of genes associated with granulosa cell proliferation and follicular development. Methods: The lncRNA and mRNA profiles of porcine granulosa cells from ATRA treatment and control group in vitro were constructed through RNA sequencing. Meanwhile, the sequencing data were verified using quantitative polymerase chain reaction (qPCR). Results: A total of 86 differentially expressed lncRNAs and 128 differentially expressed genes (DEGs) were detected in granulosa cells after ATRA treatment. The quantitative real-time PCR (qRT-PCR) results were consistent with the RNA-seq data. Functional annotation analysis revealed that the DEGs were remarkably enriched in ovary function and reproduction which contained FoxO, Hippo, Oocyte meiosis, mammalian target of rapamycin signaling pathway, as well as several pathways associated with hormone regulation like oxytocin signaling pathway and steroid hormone biosynthesis. Moreover, an interaction network of lncRNAs and their cis-target DEGs was constructed, and 7 differentially expressed lncRNAs and 6 cis-target DEGs were enriched in ovarian steroidogenesis and reproduction. Conclusion: These findings expand the lncRNA catalogue and provide a basis for further studies on the mechanism of ATRA-mediated lncRNA regulation of follicular development in pigs.

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과제정보

This research was funded by grants from the Young Scientists Fund of the National Natural Science Foundation of China (31802151), the Natural Science Foundation of Hunan Province (2024JJ5198), and the China Postdoctoral Science Foundation (2018M642981).

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