Journal of Species Research

  • 제14권4호
  • /
  • Pages.395-403
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  • 2025
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  • 2234-7909(pISSN)
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  • 2713-8615(eISSN)
국립생물자원관 (The National Institute of Biological Resources)
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New records of the marine diatom parasites Lagenisma coscinodisci (Lagenismatales, Oomycetes) and Paulsenella kornmannii (Thoracosphaerales, Dinophyceae) in Korea

  • Hyewon Kim (Major of Oceanography, Division of Earth Environmental System Science, Pukyong National University) ;
  • Sunju Kim (Major of Oceanography, Division of Earth Environmental System Science, Pukyong National University)
  • 투고 : 2025.03.13
  • 심사 : 2025.06.11
  • 발행 : 2025.11.30
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초록

This study presented the first record of two marine diatom parasites, Lagenisma coscinodisci (Oomycetes) and Paulsenella kornmannii(Dinophyceae), in Korea. Plankton samples were collected from Yongho Harbor, Busan, in March 2022, and diatoms infected by these parasites, Coscinodiscus wailesii by L. coscinodisci and Eucampia zodiacus by P. kornmannii, were isolated for morphological and molecular analyses. Both parasites exhibited developmental characteristics consistent with previous descriptions. The 18S rRNA gene sequences of L. coscinodisci (1,430 bp) and P. kornmannii (1,331 bp) were successfully obtained, with the latter being reported for the first time. Phylogenetic analysis placed L. coscinodisci within Oomycetes, clustering with Thraustotheca clavata, while P. kornmannii was positioned within Dinophyceae, closely related to P. vonstoschii. This study provides new insights into the biodiversity of parasitic protists in Korean waters and contributes the first molecular data for P. kornmannii.

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과제정보

This research was supported by a grant from the National Research Foundation (RS-2024-00351443), the Nakdonggang National Institute of Biological Resources (NNIBR 20251104), and the Honam National Institute of Biological Resources (HNIBR), funded by the Ministry of Environment(MOE) of the Republic of Korea (HNIBR202101111).

참고문헌

  1. Beakes, G.W., D. Honda and M. Thines. 2014. 3 systematics of the Straminipila: Labyrinthulomycota, Hyphochytriomycota, and Oomycota. In: McLaughlin, D.J. and Spatafora, J.W. (eds), Systematics and evolution. Springer, Berlin, Heidelberg. pp. 39-97.
  2. Buaya, A., A. Kraberg and M. Thines. 2019. Dual culture of the oomycete Lagenisma coscinodisci Drebes and Coscinodiscus diatoms as a model for plankton/parasite interactions. Helgoland Marine Research 73:2. https://doi.org/10.1186/s10152-019-0523-0
  3. Buaya, A.T. and M. Thines. 2020. An overview on the biology and phylogeny of the early-diverging oomycetes. Philippine Journal of Systematic Biology 14(1):1-20.
  4. Buaya, A.T., B. Scholz and M. Thines. 2021. A New Marine Species of Miracula (Oomycota) Parasitic to Minidiscus sp. in Iceland. Mycobiology 49(4):355-362. https://doi.org/10.1080/12298093.2021.1952813
  5. Cavalier-Smith, T. 1987. The origin of eukaryote and archaebacterial cells. Annals of the New York Academy of Sciences 503(1):17-54. https://doi.org/10.1111/nyas.1987.503.issue-1
  6. Cavalier-Smith, T. 1993. Kingdom protozoa and its 18 phyla. Microbiological Reviews 57(4):953-994. https://doi.org/10.1128/mr.57.4.953-994.1993
  7. Cavalier-Smith, T. 1998. A revised six-kingdom system of life. Biological Reviews of the Cambridge Philosophical Society 73:203-266.
  8. Cavalier-Smith, T. 1999. Principles of protein and lipid targeting in secondary symbiogenesis: euglenoid, dinoflagellate, and sporozoan plastid origins and the eukaryote family tree 1, 2. Journal of Eukaryotic Microbiology 46(4):347-366. https://doi.org/10.1111/jeu.1999.46.issue-4
  9. Cavalier-Smith, T. 2018. Kingdom Chromista and its eight phyla: a new synthesis emphasising periplastid protein targeting, cytoskeletal and periplastid evolution, and ancient divergences. Protoplasma 255:297-357. https://doi.org/10.1007/s00709-017-1147-3
  10. Chakravarty, D.K. 1974. On the ecology of the infection of the marine diatom Coscinodiscus granii by Lagenisma coscinodisci in the Weser Estuary. Veröff. Inst. Meeresforsch. Bremen, Suppl. 5:115-122.
  11. Chatton, E. 1920. Les péridiniens parasites. Morphologie, reproduction, éthologie. Archives de Zoologie Expérimentale et Générale 59:1-475.
  12. Cho, K. and S. Kim. 2024. A novel parasitoid, Pseudopirsonia chaetoceri n. sp. (Imbricatea, Cercozoa), of marine diatoms discovered in Korean coastal waters. Algae 39(4):225-239. https://doi.org/10.4490/algae.2024.39.11.15
  13. Danz, A. and C.A. Quandt. 2023. A review of the taxonomic diversity, host-parasite interactions, and experimental research on chytrids that parasitize diatoms. Frontiers in Microbiology 14:1281648.
  14. Drebes, G. 1966. Ein parasitischer Phycomycet (Lagenidiales) in Coscinodiscus. Helgoliinder Wiss. Meeresunters 13: 426-435. https://doi.org/10.1007/BF01611959
  15. Drebes, G. 1968. Lagenisma coscinodisci. General nov spec. nov., ein Vertreter der Lagenidiales in der marinen Diatomee Coscinodiscus. Veroffentl. Inst Meeresf. Bremerhaven 3:67-70.
  16. Drebes, G. 1974. Marines Phytoplankton. Thierne, Stuttgart, 186.
  17. Drebes, G. and E. Schnepf. 1988. Paulsenella Chatton (Dinophyta), ectoparasites of marine diatoms: development and taxonomy. Helgoländer Meeresuntersuchungen 42:563-581. https://doi.org/10.1007/BF02365627
  18. Dumack, K., S. Flues, K. Hermanns and M. Bonkowski. 2017. Rhogostomidae (Cercozoa) from soils, roots and plant leaves (Arabidopsis thaliana): Description of Rhogostoma epiphylla sp. nov. and R. cylindrica sp. nov. European Journal of Protistology 60:76-86. https://doi.org/10.1016/j.ejop.2017.06.001
  19. Ehrenberg, C.G. 1839. Über jetzt wirklich noch zahlreich lebende Thierarten der Kreidebildung und den Organismus der Polythalamien. Abhandlungen der königlichen Akademie der Wissenschaften zu, Berlin 1839:81-174.
  20. Frances, S.P., A.W. Sweeney and R.A. Humber. 1989. Crypticola clavulifera gen. et sp. nov. and Lagenidium giganteum: Oomycetes pathogenic for dipterans infesting leaf axils in an Australian rain forest. Journal of Invertebrate Pathology 54(1):103-111. https://doi.org/10.1016/0022-2011(89)90146-8
  21. Fritz, L. and R.E. Triemer. 1985. A rapid simple technique utilizing calcofluor white M2R for the visualization of dinoflagellate thecal plates. Journal of Phycology 21(4):662-664. https://doi.org/10.1111/jpy.1985.21.issue-4
  22. Giovannoni, S.J., E.F. DeLong, G.J. Olsen and N.R. Pace. 1988. Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells. Journal of Bacteriology 170(2):720-726. https://doi.org/10.1128/jb.170.2.720-726.1988
  23. Gotelli, D. 1971. Lagenisma coscinodisci, a parasite of the marine diatom Coscinodiscus occurring in the Puget Sound, Washington. Mycologia 63(1):171-174. https://doi.org/10.1080/00275514.1971.12019095
  24. Grahame, E.S. 1976. The occurrence of Lagenisma coscinodisci in Palmeria hardmaniana from Kingston Harbour, Jamaica. British Phycological Journal 11(1):57-61. https://doi.org/10.1080/00071617600650091
  25. Gran, H.H. and E.C. Angst. 1931. Plankton diatoms of Puget Sound. Publications of the Puget Sound Biological Station 7:417-516.
  26. Guillard, R.R.L. and J.H. Ryther. 1962. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran. Canadian Journal of Microbiology 8(2):229-239. https://doi.org/10.1139/m62-029
  27. Katoh, K. and D.M. Standley. 2013. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4):772-780. https://doi.org/10.1093/molbev/mst010
  28. Katoh, K., J. Rozewicki and K.D. Yamada. 2019. MAFFT online service: multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4):1160-1166. https://doi.org/10.1093/bib/bbx108
  29. Kim, S. and M.G. Park. 2014. Amoebophrya spp. from the bloom-forming dinoflagellate Cochlodinium polykrikoides: parasites not nested in the “Amoebophrya ceratii complex”. Journal of Eukaryotic Microbiology 61:173-181. https://doi.org/10.1111/jeu.2014.61.issue-2
  30. Konovalova, G.V. 2008. Parasitic dinoflagellates and ellobiopsids (Ellobiopsidae) of the coastal waters of the Sea of Japan. Russian Journal of Marine Biology 34:28-37. https://doi.org/10.1134/S1063074008010045
  31. Kühn, S.F. 1997. Victoriniella multiformis, gen. et spec. nov. (incerta sedis), a polymorphic parasitoid protist infecting the marine diatom Coscinodiscus wailesii Gran & Angst (North Sea, German Bight). Archiv für Protistenkunde 148(1-2):115-123. https://doi.org/10.1016/S0003-9365(97)80039-5
  32. Kühn, S.F. and L.K. Medlin. 2005. The systematic position of the parasitoid marine dinoflagellate Paulsenella vonstoschii (Dinophyceae) inferred from nuclear-encoded small subunit ribosomal DNA. Protist 156(4):393-398. https://doi.org/10.1016/j.protis.2005.09.002
  33. Larousse, M. and E. Galiana. 2017. Microbial partnerships of pathogenic oomycetes. PLoS Pathogens 13(1):e1006028. https://doi.org/10.1371/journal.ppat.1006028
  34. Lee, S.J., M.S. Hwang, M. Park, J.M. Baek, D.S. Ha, J.E. Lee and S.R. Lee. 2015. Molecular identification of the algal pathogen Pythium chondricola (Oomycetes) from Pyropia yezoensis (Rhodophyta) using ITS and cox1 markers. Algae 30(3):217-222. https://doi.org/10.4490/algae.2015.30.3.217
  35. Mendoza, L., R. Vilela and R.A. Humber. 2018. Taxonomic and phylogenetic analysis of the Oomycota mosquito larvae pathogen Crypticola clavulifera. Fungal Biology 122(9): 847-855. https://doi.org/10.1016/j.funbio.2018.04.010
  36. Michael, W. Dick. 2001. Straminipilous fungi. Kluwer, Academic Publishers, Dordrecht.
  37. Moon-van der Staay, S.Y., G.W. van der Staay, L. Guillou, D. Vaulot, H. Claustre and L.K. Medlin. 2000. Abundance and diversity of prymnesiophytes in the picoplankton community from the equatorial Pacific Ocean inferred from 18S rDNA sequences. Limnology and Oceanography 45(1):98-109. https://doi.org/10.4319/lo.2000.45.1.0098
  38. Parsons, T.R. 1962. Infection of a Marine Diatom by Lagenidium sp. Canadian Journal of Botany 40(3):523-523. https://doi.org/10.1139/b62-048
  39. Pascher, A. 1914. Über Flagellaten und Algen. Archiv für Protistenkunde 33:411-552.
  40. Paulsen, O. 1911. Marine plankton from the East-Greenland Sea. III. Peridiniales. - Danm. Eksped. Grønlands Nordøstkyst 1906-1908 3(11):303-318.
  41. Purushothaman, A., L.C. Thomas and K.B. Padmakumar. 2023. Planktoniella sol, a New Host for the Ectoparasitic Dinophyta Paulsenella Chatton from the Northeastern Arabian Sea. Thalassas: An International Journal of Marine Sciences 39(2):651-656. https://doi.org/10.1007/s41208-023-00545-6
  42. Ruggiero,M.A.,D.P. Gordon,T.M. Orrell,NaBllyT. Bour-goin,R.C.Brusca,T.Cavalier-Smith,M.D.Guiry andP.M. Kirk. 2015.Ahigher level classification of all living organ-isms. PLoS One 10(4):e0119248. https://doi.org/10.1371/journal.pone.0119248
  43. Schiller, J. 1930. Coccolithineae. In: Rabenhorst, L. (ed.), Kryptogamen-Flora von Deutschland, Österreich und der Schweiz. Zehnter Band. Flagellatae. Akademische Verlagsgesellschaft, Leipzig. pp. 89-273.
  44. Schnepf, E. and G. Drebes. 1977. Über die Entwicklung des marinen parasitischen Phycomyceten Lagenisma coscinodisci (Lagenidiales). Helgoländer Wissenschaftliche Meeresuntersuchungen 29:291-301. https://doi.org/10.1007/BF01614265
  45. Schnepf, E., G. Drebes and M. Elbrächter. 1990. Pirsonia guinardiae, gen. et spec. nov.: a parasitic flagellate on the marine diatom Guinardia flaccida with an unusual mode of food uptake. Helgoländer Meeresuntersuchungen 44:275-293. https://doi.org/10.1007/BF02365468
  46. Schweikert, M. and E. Schnepf. 1996. Pseudaphelidium drebesii, gen et spec nov (incerta sedis), a Parasite of the Marine Centric Diatom Thalassiosira punctigera. Archiv für Protistenkunde 147(1):11-17. https://doi.org/10.1016/S0003-9365(96)80004-2
  47. Scholz, B., L. Guillou, A.V. Marano, S. Neuhauser, B.K. Sullivan, U. Karsten, F.C. Küpper and F.H. Gleason. 2016. Zoosporic parasites infecting marine diatoms - a black box that needs to be opened. Fungal Ecology 19:59-76. https://doi.org/10.1016/j.funeco.2015.09.002
  48. Stamatakis, A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics 30:1312-1313. https://doi.org/10.1093/bioinformatics/btu033
  49. Tamura, K., G. Stecher and S. Kumar. 2021. MEGA11: molecular evolutionary genetics analysis version 11. Molecular Biology and Evolution 38:3022-3027. https://doi.org/10.1093/molbev/msab120
  50. Tangen, K., L.E. Brand, P.L. Blackwelder and R.R. Guillard. 1982. Thoracosphaera heimii (Lohmann) Kamptner is a dinophyte: observations on its morphology and life cycle. Marine Micropaleontology 7(3):193-212. https://doi.org/10.1016/0377-8398(82)90002-0
  51. Wetsteyn, L.P.M.J. and L. Peperzak. 1991. Field observations in the Oosterschelde (The Netherlands) on Coscinodiscus concinnus and Coscinodiscus granii (Bacillariophyceae) infected by the marine fungus Lagenisma coscinodisci (Oomycetes). Hydrobiological Bulletin 25:15-21. https://doi.org/10.1007/BF02259584
  52. Winter, G. 1880. Rabenhorst’s Kryptogamen-Flora, Pilze Schizomyceten, Saccharomyceten und Basidiomyceten, Vol. 1, 2nd ed. E. Kummer, Leipzig.
  53. Yoo, J. and S. Kim. 2020. Infection of marine diatom Coscinodiscus wailesii (Bacillariophyceae) by the parasitic nanoflagellate Pirsonia diadema (Stramenopiles) from Yongho Bay in Korea. Korean Journal of Environmental Biology 38(4):567-577. https://doi.org/10.11626/KJEB.