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Avantor Performance Materials Korea (아반토퍼포먼스머티리얼즈코리아)
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A practical guide to maximizing sample peak capacity for complex low molecular mass molecule separations.

복잡한 저분자량 분자 분리를 위한 시료 피크 용량 극대화 가이드

  • Arianne Soliven (Avantor Korea) ;
  • Matt James (Avantor Korea) ;
  • Tony Edge (Avantor Korea)
  • Published : 2024.06.26
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Abstract

Method development for complex low molecular mass (LMM) samples using reversed-phase (RP) separation conditions presents significant challenges due to the presence of many unknown analytes over wide concentration ranges. This guide aims to optimize method parameters-column length (L), temperature (T), flow rate (F), and final mobile phase conditions (Øfinal)-to maximize separation peak capacity. Validated by prior research, this protocol benefits laboratories dealing with metabolomics, natural products, and contaminant screening. This practical guide provides a structured approach to maximizing peak capacity for complex LMM separations. It complements computational optimization strategies and offers a step-by-step method development process. The Snyder-Dolan test is highlighted as essential for determining the need for gradient or isocratic elution and guiding column length decisions. The decision tree framework helps analysts prioritize variable optimization to develop effective separation methods for complex samples.

Keywords

References

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