Journal of Microbiology and Biotechnology

  • 제34권1호
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  • Pages.103-115
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Evaluation of the Efficacy of a Lactobacilli-Based Teat Detergents for the Microbiota of Cows Teats Using an Untargeted Metabolomics Approach

  • Rui Yan (Inner Mongolia Key Laboratory for Biomass-Energy Conversion, Inner Mongolia University of Science and Technology) ;
  • Zhongqing Ji (School of Life Science and Technology, Inner Mongolia University of Science and Technology) ;
  • Jiaqi Fan (School of Life Science and Technology, Inner Mongolia University of Science and Technology) ;
  • Jiang Li (School of Life Science and Technology, Inner Mongolia University of Science and Technology) ;
  • Yan Ren (Inner Mongolia Key Laboratory for Biomass-Energy Conversion, Inner Mongolia University of Science and Technology)
  • 투고 : 2023.05.18
  • 심사 : 2023.09.08
  • 발행 : 2024.01.28
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초록

Teat cleaning pre- and post-milking is important for the overall health and hygiene of dairy cows. The purpose of this research was to evaluate the effectiveness of a teat detergents based on lactic acid bacteria according to changes in somatic cell count and cow-milk metabolites. Sixty-nine raw milk samples were collected from 11 Holstein-Friesian cows in China during 12 days of teat cleaning. An ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry-based untargeted metabolomic approach was applied to detect metabolomic differences after treatment with lactic acid bacteria and chemical teat detergents in cows with subclinical mastitis. The results suggest that the lactobacilli-based teat detergents could reduce somatic cell count and improve microhabitat of cow teat apex by adjusting the composition of metabolites. Furthermore, the somatic cell count could be decreased significantly within 10 days following the cleaning protocol. Lactic acid bacteria have the potential to be applied as a substitution to teat chemical detergents before and after milking for maintenance of healthy teats and breasts. Further, larger scale validation work is required to support the findings of the current study.

키워드

과제정보

This study was supported by the Inner Mongolia Autonomous Region Natural Science Fund (No.2020BS03009). We also thank Inner Mongolia Agricultural University and Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education for donating Lactobacillus plantarum strains.

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