Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Dietary phytosterols improves the metabolic status of perinatal cows as evidenced by plasma metabolomics and faecal microbial metabolism

  • Jian Gao (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Donghai Lv (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Zichen Wu (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Zhanying Sun (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Xiaoni Sun (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Suozhu Liu (College of Animal Science, Tibet Agricultural and Husbandry University) ;
  • Zhankun Tan (College of Animal Science, Tibet Agricultural and Husbandry University) ;
  • Weiyun Zhu (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University) ;
  • Yanfen Cheng (Laboratory of Gastrointestinal Microbiology, National Centre for International Research on Animal Gut Nutrition, Nanjing Agricultural University)
  • Received : 2023.10.16
  • Accepted : 2024.03.28
  • Published : 2024.10.01
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Abstract

Objective: Previous research reported that dietary addition with phytosterols improved the energy utilisation of the rumen microbiome, suggesting its potential to alleviate the negative energy balance of perinatal cows. This experiment aimed to explore the effects of feeding phytosterols on the metabolic status of perinatal cows through plasma metabolomics and faecal bacteria metabolism. Methods: Ten perinatal Holstein cows (multiparous, 2 parities) with a similar calving date were selected four weeks before calving. After 7 days for adaptation, cows were allocated to two groups (n = 5), which respectively received the basal rations supplementing commercial phytosterols at 0 and 200 mg/d during a 42-day experiment. The milk yield of each cow was recorded daily after calving. On days 1 and 42, blood and faeces samples were all collected from perinatal cows before morning feeding for analysing plasma biochemicals and metabolome, and faecal bacteria metabolism. Results: Dietary addition with phytosterols at 200 mg/d had no effects on plasma cholesterol and numerically increased milk yield by 1.82 kg/d (p>0.10) but attenuated their negative energy balance in perinatal cows as observed from the significantly decreased plasma level of β-hydroxybutyric acid (p = 0.002). Dietary addition with phytosterols significantly altered 12 and 15 metabolites (p<0.05) within the plasma and faeces of perinatal cows, respectively. Of these metabolites, 5 upregulated plasma fatty acids indicated an improved energy status (i.e., C18:1T, C14:0, C17:0, C18:0, and C16:0). Milk yield negatively correlated with plasma concentrations of ketone bodies (p = 0.035) and 5-methoxytryptamine (p = 0.039). Furthermore, dietary addition with phytosterols at 200 mg/d had no effects on fermentation characteristics and bacterial diversity of cow faeces (p>0.10) but improved potentially beneficial bacteria such as Christensenellaceae family (p<0.05) that positively correlated with feed efficiency. Conclusion: Dietary addition with phytosterols at 200 mg/d could effectively improve the energy status in perinatal cows by attenuating their negative energy balance.

Keywords

Acknowledgement

The authors gave special thanks to Shanghai Biotree Biotech Co., Ltd. for the technical support for the metabolomics analyses.

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