Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
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Immuno-enhancing effect of Codium fragile extracts in RAW264.7 macrophages

  • Seul Ah Lee (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Jin Shil Choi (Department of Oral Biochemistry, College of Dentistry, Chosun University) ;
  • Chun Sung Kim (Department of Oral Biochemistry, College of Dentistry, Chosun University)
  • Received : 2024.06.23
  • Accepted : 2024.07.30
  • Published : 2024.10.31
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Abstract

Codium fragile (CF) is a green seaweed that has been used in folk medicine to treat urinary tract disorders, intestinal bacteriosis, and dropsy. The CF is also used primarly as a seasoning to enhance the flavor of Kimchi, and in some areas it is consumed as a side dish. The CF has reported to have anti-coagulant, thrombolytic, anti-inflammatory, anti-oxidant activities. The purpose of this study was to analyze the immune-enhancing effect of aqueous extract of C. fragile (AECF) using RAW264.7 macrophages and splenocytes. The AECF induced the production of nitrite oxide and inducible nitric oxide synthase at 1 mg/mL without cytotoxicity, and also gradually increased the expression of pro-inflammatory cytokines (tumor necrosis factor-alpha and interleukin-6), determined by griess reagent, western blot, and Enzyme-linked immunosorbent assay. In addition, AECF significantly induced the expression of immune-related enzymes, granulocyte-macrophage colony-stimulating factor, granulocyte colony-stimulating factor, and monocyte chemoattracted protein-1, which increase the production of immune cells, determined by mouse cytokine array, as determined by cytokine arrray. The AECF induced phosphorylation of mitogen-activated protein kinases and translocation of nuclear factor kappa B p65 subunit from cytosol to the nucleus. Our findings indicate that C. fragile may have potential as a functional ingredinet for developing immune-enhancing supplement that properly maintiain the body's defense system.

Keywords

Acknowledgement

This study was supported by a research from Chosun University, 2021.

References

  1. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular biology of the cell. 4th ed. New York, NY: Garland Science; 2002.
  2. Apetoh L, Ghiringhelli F, Tesniere A, Obeid M, Ortiz C, Criollo A, et al. Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy. Nat Med. 2007;13:1050-9. https://doi.org/10.1038/nm1622
  3. Bonnardel J, Da Silva C, Henri S, Tamoutounour S, Chasson L, Montanana-Sanchis F, et al. Innate and adaptive immune functions of peyer's patch monocyte-derived cells. Cell Rep. 2015;11:770-84. https://doi.org/10.1016/j.celrep.2015.03.067
  4. Cekici A, Kantarci A, Hasturk H, van Dyke TE. Inflammatory and immune pathways in the pathogenesis of periodontal disease. Periodontology. 2000;64:57-80. https://doi.org/10.1111/prd.12002
  5. Chen Y, Fang ZM, Yi X, Wei X, Jiang DS. The interaction between ferroptosis and inflammatory signaling pathways. Cell Death Dis. 2023;14:205.
  6. Choi JH, Sapkota K, Park SE, Kim S, Kim SJ. Thrombolytic, anticoagulant and antiplatelet activities of codiase, a bi-functional fibrinolytic enzyme from Codium fragile. Biochimie. 2013;95:1266-77. https://doi.org/10.1016/j.biochi.2013.01.023
  7. Drazen DL, Bilu D, Bilbo SD, Nelson RJ. Melatonin enhancement of splenocyte proliferation is attenuated by luzindole, a melatonin receptor antagonist. Am J Physiol Regul Integr Comp Physiol. 2001;280:R1476-82. https://doi.org/10.1152/ajpregu.2001.280.5.R1476
  8. Echave J, Fraga-Corral M, Garcia-Perez P, Popovic-Djordjevic J, Avdovic EH, Radulovic M, et al. Seaweed protein hydrolysates and bioactive peptides: extraction, purification, and applications. Mar Drugs. 2021;19:500.
  9. Elmore SA. Enhanced histopathology of the spleen. Toxicol Pathol. 2006;34:648-55. https://doi.org/10.1080/01926230600865523
  10. Feng H, Fan J, Lin L, Liu Y, Chai D, Yang J. Immunomodulatory effects of phosphorylated Radix cyathulae officinalis polysaccharides in immunosuppressed mice. Molecules. 2019;24:4150.
  11. Haftcheshmeh SM, Abedi M, Mashayekhi K, Mousavi MJ, Navashenaq JG, Mohammadi A, et al. Berberine as a natural modulator of inflammatory signaling pathways in the immune system: focus on NF-κB, JAK/STAT, and MAPK signaling pathways. Phytother Res. 2022;36:1216-30. https://doi.org/10.1002/ptr.7407
  12. Jo KA, Kim KJ, Park S, Jeon JY, Hwang JE, Kim JY. Evaluation of the effects of Euglena gracilis on enhancing immune responses in RAW264.7 cells and a cyclophosphamide-induced mouse model. J Microbiol Biotechnol. 2023;33:493-9. https://doi.org/10.4014/jmb.2212.12041
  13. Kang CH, Choi YH, Park SY, Kim GY. Anti-inflammatory effects of methanol extract of Codium fragile in lipopolysaccharide-stimulated RAW 264.7 cells. J Med Food. 2012;15:44-50. https://doi.org/10.1089/jmf.2010.1540
  14. Khan AA, Manzoor KN, Sultan A, Saeed M, Rafique M, Noushad S, et al. Pulling the brakes on fast and furious multiple drug-resistant (MDR) bacteria. Int J Mol Sci. 2021;22:859.
  15. Khatua S, Simal-Gandara J, Acharya K. Understanding immune-modulatory efficacy in vitro. Chem Biol Interact. 2022;352:109776.
  16. Koyanagi S, Tanigawa N, Nakagawa H, Soeda S, Shimeno H. Oversulfation of fucoidan enhances its anti-angiogenic and antitumor activities. Biochem Pharmacol. 2003;65:173-9. https://doi.org/10.1016/S0006-2952(02)01478-8
  17. Lee JB, Ohta Y, Hayashi K, Hayashi T. Immunostimulating effects of a sulfated galactan from Codium fragile. Carbohydr Res. 2010;345:1452-4. https://doi.org/10.1016/j.carres.2010.02.026
  18. Li B, Lu F, Wei X, Zhao R. Fucoidan: structure and bioactivity. Molecules. 2008;13:1671-95. https://doi.org/10.3390/molecules13081671
  19. Lomartire S, Goncalves AMM. An overview of potential seaweed-derived bioactive compounds for pharmaceutical applications. Mar Drugs. 2022;20:141.
  20. Lv R, Bao Q, Li Y. Regulation of M1-type and M2-type macrophage polarization in RAW264. 7 cells by Galectin-9. Mol Med Rep. 2017;16:9111-9. https://doi.org/10.3892/mmr.2017.7719
  21. Monmai C, Rod-In W, Jang A, Lee S, Jung SK, You S, et al. Immune-enhancing effects of anionic macromolecules extracted from Codium fragile coupled with arachidonic acid in RAW264.7 cells. PLOS ONE. 2020;15:e0239422.
  22. Monmai C, You S, Park WJ. Immune-enhancing effects of anionic macromolecules extracted from Codium fragile on cyclophosphamide-treated mice. PLOS ONE. 2019;14:e0211570.
  23. Moon SM, Lee SA, Han SH, Park BR, Choi MS, Kim JS, et al. Aqueous extract of Codium fragile alleviates osteoarthritis through the MAPK/NF-κB pathways in IL-1β-induced rat primary chondrocytes and a rat osteoarthritis model. Biomed Pharmacother. 2018;97:264-70. https://doi.org/10.1016/j.biopha.2017.10.130
  24. Mrityunjaya M, Pavithra V, Neelam R, Janhavi P, Halami PM, Ravindra PV. Immune-boosting, antioxidant and anti-inflammatory food supplements targeting pathogenesis of COVID-19. Front Immunol. 2020;11:570122.
  25. Netea MG, Quintin J, van der Meer JWM. Trained immunity: a memory for innate host defense. Cell Host Microbe. 2011;9:355-61. https://doi.org/10.1016/j.chom.2011.04.006
  26. Netea MG, Schlitzer A, Placek K, Joosten LAB, Schultze JL. Innate and adaptive immune memory: an evolutionary continuum in the host's response to pathogens. Cell Host Microbe. 2019;25:13-26. https://doi.org/10.1016/j.chom.2018.12.006
  27. Omara FO, Blakley BR, Bernier J, Fournier M. Immunomodulatory and protective effects of N-acetylcysteine in mitogen-activated murine splenocytes in vitro. Toxicology. 1997;116:219-26. https://doi.org/10.1016/S0300-483X(96)03520-2
  28. Park HB, Hwang J, Zhang W, Go S, Kim J, Choi I, et al. Polysaccharide from Codium fragile induces anti-cancer immunity by activating natural killer cells. Mar Drugs. 2020;18:626.
  29. Pezzanite LM, Chow L, Johnson V, Griffenhagen GM, Goodrich L, Dow S. Toll-like receptor activation of equine mesenchymal stromal cells to enhance antibacterial activity and immunomodulatory cytokine secretion. Vet Surg. 2021;50:858-71. https://doi.org/10.1111/vsu.13628
  30. Seo YS, Shin KS. Immune system-stimulating activities of mucilage polysaccharides isolated from Opuntia humifusa. J Korean Soc Food Sci Nutr. 2012;41:95-102. https://doi.org/10.3746/jkfn.2012.41.1.095
  31. Surayot U, You S. Structural effects of sulfated polysaccharides from Codium fragile on NK cell activation and cytotoxicity. Int J Biol Macromol. 2017;98:117-24. https://doi.org/10.1016/j.ijbiomac.2017.01.108
  32. Uthaisangsook S, Day NK, Bahna SL, Good RA, Haraguchi S. Innate immunity and its role against infections. Ann Allergy Asthma Immunol. 2002;88:253-65. https://doi.org/10.1016/S1081-1206(10)62005-4
  33. Yang J, Jeon H, You YH, Kim JY, Choi HK, Choi KC, et al. Citrus ethanol extracts promotes innate immune response by activating NF-κB. J Korean Soc Food Sci Nutr. 2015;44:1256-63. https://doi.org/10.3746/jkfn.2015.44.9.1256
  34. Zhao P, Cao L, Wang X, Li J, Dong J, Zhang N, et al. Giardia duodenalis extracellular vesicles regulate the proinflammatory immune response in mouse macrophages in vitro via the MAPK, AKT and NF-κB pathways. Parasit Vectors. 2021;14:358.