한국식생활문화학회지 (Journal of the Korean Society of Food Culture)

  • 제39권5호
  • /
  • Pages.268-274
  • /
  • 2024
  • /
  • 1225-7060(pISSN)
  • /
  • 2288-7148(eISSN)
한국식생활문화학회 (Korean Society of Food Culture)
권호 표지
DOI QR코드

DOI QR Code

쌍별귀뚜라미의 용매분획별 항산화 활성과 피부세포주 보호 효과

The Antioxidant Activity and Protective Effects on Skin Cell Lines of Solvent Fractions from Gryllus bimaculatus

  • 김경 (을지대학교 식품영양학과) ;
  • 오윤신 (을지대학교 식품영양학과)
  • Kyong Kim (Department of Food and Nutrition, Eulji University) ;
  • Yoon Sin Oh (Department of Food and Nutrition, Eulji University)
  • 투고 : 2024.09.20
  • 심사 : 2024.10.31
  • 발행 : 2024.10.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Oxidative stress caused by reactive oxygen species (ROS) is a key mechanism of skin aging, and the use of antioxidants is an effective strategy to prevent the symptoms associated with ROS-induced skin aging. The components rich in polyphenolic compounds with antioxidative activity were identified by fractionating an aqueous ethanolic extract of Gryllus bimaculatus (AE-GBE) using solvent using hexane, chloroform, ethyl acetate, butanol, and water. Their ability to migrate H2O2-induced oxidative stress in human dermal fibroblasts (HDFs) was then evaluated. The butanol fraction of AE-GBE had the highest polyphenol content and antioxidant effect, followed by the ethyl acetate and water fractions, suggesting that the likely antioxidant components are polar components. Furthermore, the butanol, ethyl acetate, and water fractions effectively reduced intracellular ROS production and DNA damage in HDF cells caused by H2O2. Overall, these findings suggest that the butanol fraction of AE-GBE shows promise as a natural insect-derived antioxidant material, capable of suppressing oxidative stress by showing a stronger antioxidant effect under H2O2 stimulation than the other fractions.

키워드

과제정보

본 논문은 2024년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(NO. RS-2024-00336513)을 받아 수행된 결과의 일부로, 이에 깊은 감사를 드립니다.

참고문헌

  1. Ahn MY, Hwang JS, Yun EY, Kim MJ, Park KK. 2015. Anti-aging Effect and Gene Expression Profiling of Aged Rats Treated with G. bimaculatus Extract. Toxicol. Res., 31(2):173-180
  2. Ainsworth EA, Gillespie KM. 2007. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent. Nat. Protoc., 2(4):875-877
  3. Beckman JS, Minor RL, White CW, Repine JE, Rosen GM, Freeman BA. 1988. Superoxide dismutase and catalase conjugated to polyethylene glycol increases endothelial enzyme activity and oxidant resistance. J. Biol. Chem., 263(14):6884-6892
  4. Blois MS. 1958. Antioxidant Determinations by the Use of a Stable Free Radical. Nat., 181(4617):1199-1200
  5. Branen AL. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil. Chem. Soc., 52(2):59-63
  6. Chong HS, Kim SY, Cho SR, Park HI, Baek JE, Kuk JS, Suh HJ. 2017. Characteristics of Quality and Antioxidant Activation of the Cookies adding with Mealworm (Tenebrio molitor) and Black Bean Powder. J. Food Saf. Hyg., 32(6):521-530
  7. Di Mattia C, Battista N, Sacchetti G, Serafini M. 2019. Antioxidant Activities in vitro of Water and Liposoluble Extracts Obtained by Different Species of Edible Insects and Invertebrates. Front Nutr., 6:106
  8. Dzialo M, Mierziak J, Korzun U, Preisner M, Szopa J, Kulma A. 2016. The Potential of Plant Phenolics in Prevention and Therapy of Skin Disorders. Int. J. Mol. Sci., 17(2):160
  9. Farag RS, Badei AZMA, El Baroty GSA. 1989. Influence of thyme and clove essential oils on cottonseed oil oxidation. J. Am. Oil. Chem. Soc., 66(6):800-804
  10. Fashakin OO, Tangjaidee P, Unban K, Klangpetch W, Khumsap T, Sringarm K, Rawdkuen S, Phongthai S. 2023. Isolation and Identification of Antioxidant Peptides Derived from Cricket (Gryllus bimaculatus) Protein Fractions. Insects, 14:674
  11. Frippiat C, Chen QM, Zdanov S, Magalhaes JP, Remacle J, Toussaint O. 2001. Subcytotoxic H2O2 stress triggers a release of transforming growth factor-beta 1, which induces biomarkers of cellular senescence of human diploid fibroblasts. J. Biol. Chem., 276(4):2531-2537
  12. Han JS. 2021. Nutritional Value and Anti-inflammation Activity of Misutkaru with Added Gryllus bimaculatus Powder. Asian J. Beauty Cosmetol., 19(3):467-476
  13. Hwang BB, Chang MH, Lee JH, Heo W, Kim JK, Pan JH, Kim YJ, Kim JH. 2019. The Edible Insect Gryllus bimaculatus Protects against Gut-Derived Inflammatory Responses and Liver Damage in Mice after Acute Alcohol Exposure. Nutr., 11(4):
  14. Jang HY, Park CE, Lee SO. 2019. Comparison of antioxidant capacity of protein hydrolysates from 4 different edible insects. Korean J. Food Sci. Technol., 51(5):480-485
  15. Kim HJ, Mun J, Kang JS, Oh SH, Kim JH. 2024a. Antioxidant activity of the ethyl acetate fraction of Gryllus bimaculatus in Caenorhabditis elegans. Korean J. Food Sci. Technol., 56(1):46-54
  16. Kim K, Kim C-E, Baek D-J, Park E-Y, Oh YS. 2024b. Prevention of UVB-Induced Photoaging by an Ethyl Acetate Fraction from Allomyrina dichotoma Larvae and Its Potential Mechanisms in Human Dermal Fibroblasts. Int. J. Mol., 25(14):7850
  17. Kim K, Lee C, Park E, Oh Y. 2023. Anti-skinaging effects of Gryllus bimaculatus on ERM-CZ100-exposed human diploid fibroblasts. J. Nutr. Health, 56(6):615-628
  18. Kim SH, Kim YK, Han JS. 2020. Antioxidant Activities and Nutritional Components of Cricket (Gryllus bimaculatus) Powder and Protein Extract. Asian J. Beauty Cosmetol., 18(2):163-172
  19. Ku HB, Kim GN. 2023. Protective Effect of Gryllus bimaculatus Extract on Ethanol-induced HepG2 Cells. J. East Asian Soc. Diet. Life, 33(3):212-220
  20. Lee SE, Lee JH, Kim JK, Kim GS, Kim YO, Soe JS, Choi JH, Lee ES, Noh HJ, Kim SY. 2011. Anti-inflammatory Activity of Medicinal Plant Extracts. Korean J. Med. Crop. Sci., 19(4):217-226
  21. Lim HJ, Byun EH. 2021. Evaluation of Anti-Cancer Activity of Gryllus bimaculatus Water Extract on Non-Small Cancer Lung Cell via Apoptosis. Prev. Nutr. Food Sci., 26(4):453-458
  22. Liu HW, Wang LL, Tang X, Dong ZM, Guo PC, Zhao DC, Xia QY, Zhao P. 2018. Proteomic analysis of Bombyx mori molting fluid: Insights into the molting process. J. Proteom., 173:115-125
  23. Manach C, Williamson G, Morand C, Scalbert A, Remesy C. 2005. Bioavailability and bioefficacy of polyphenols in humans. I. Review of 97 bioavailability studies. Am. J. Clin. Nutr., 81(1 suppl):230s-242s
  24. Mukherjee S, Date A, Patravale V, Korting HC, Roeder A, Weindl G. 2006. Retinoids in the treatment of skin aging: an overview of clinical efficacy and safety. Clin. Interv. Aging, 1(4):327-348
  25. Nino MC, Reddivari L, Osorio C, Kaplan I, Liceaga A. 2021. Insects as a source of phenolic compounds and potential health benefits. J. Insects Food Feed, 7(7):1077-1087
  26. Osborn-Barnes HT, Akoh CC. 2003. Effects of alpha-tocopherol, beta-carotene, and soy isoflavones on lipid oxidation of structured lipid-based emulsions. J. Agric. Food Chem., 51(23):6856-6860
  27. Park C, Cha HJ, Hong SH, Kim GY, Kim Sm, Kim HS, Kim BW, Jeon YJ, Choi YH. 2019. Protective Effect of Phloroglucinol on Oxidative Stress-Induced DNA Damage and Apoptosis through Activation of the Nrf2/HO-1 Signaling Pathway in HaCaT Human Keratinocytes. Mar. Drugs, 17(4):225
  28. Park S, Yun E. 2018. Edible Insect Food: Current Scenario and Future Perspectives. Food Sci. Anim. Resour., 7(1):12-20
  29. Remacle J, Michiels C, Raes M. 1992. The importance of antioxidant enzymes in cellular aging and degeneration. Exs, 62:99-108
  30. Rittie L, Fisher GJ. 2002. UV-light-induced signal cascades and skin aging. Ageing Res. Rev., 1(4):705-720
  31. Rizvi SI, Maurya PK. 2007. Alterations in antioxidant enzymes during aging in humans. Mol. Biotechnol., 37(1):58-61
  32. Seifried HE, Anderson DE, Fisher EI, Milner JA. 2007. A review of the interaction among dietary antioxidants and reactive oxygen species. J. Nutr. Biochem., 18(9):567-579
  33. Vistica DT, Skehan P, Scudiero D, Monks A, Pittman A, Boyd MR. 1991. Tetrazolium-based assays for cellular viability: a critical examination of selected parameters affecting formazan production. Cancer Res., 51(10):2515-2520
  34. Wu X, He K, Velickovic TC, Liu Z. 2021. Nutritional, functional, and allergenic properties of silkworm pupae. Food Sci. Nutr., 9(8):4655-4665
  35. Xia Y, Bamdad F, Ganzle M, Chen L. 2012. Fractionation and characterization of antioxidant peptides derived from barley glutelin by enzymatic hydrolysis. Food Chem., 134:1509-1518
  36. Zielinska E, Karas M, Jakubczyk A. 2017. Antioxidant activity of predigested protein obtained from a range of farmed edible insects. Int. J. Food Sci. Technol., 52:306-312