Journal of the Korean Society of Food Culture (한국식생활문화학회지)

Korean Society of Food Culture (한국식생활문화학회)
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The Antioxidant Activity and Protective Effects on Skin Cell Lines of Solvent Fractions from Gryllus bimaculatus

쌍별귀뚜라미의 용매분획별 항산화 활성과 피부세포주 보호 효과

  • Kyong Kim (Department of Food and Nutrition, Eulji University) ;
  • Yoon Sin Oh (Department of Food and Nutrition, Eulji University)
  • 김경 (을지대학교 식품영양학과) ;
  • 오윤신 (을지대학교 식품영양학과)
  • Received : 2024.09.20
  • Accepted : 2024.10.31
  • Published : 2024.10.31
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Abstract

Oxidative stress caused by reactive oxygen species (ROS) is a key mechanism of skin aging, and the use of antioxidants is an effective strategy to prevent the symptoms associated with ROS-induced skin aging. The components rich in polyphenolic compounds with antioxidative activity were identified by fractionating an aqueous ethanolic extract of Gryllus bimaculatus (AE-GBE) using solvent using hexane, chloroform, ethyl acetate, butanol, and water. Their ability to migrate H2O2-induced oxidative stress in human dermal fibroblasts (HDFs) was then evaluated. The butanol fraction of AE-GBE had the highest polyphenol content and antioxidant effect, followed by the ethyl acetate and water fractions, suggesting that the likely antioxidant components are polar components. Furthermore, the butanol, ethyl acetate, and water fractions effectively reduced intracellular ROS production and DNA damage in HDF cells caused by H2O2. Overall, these findings suggest that the butanol fraction of AE-GBE shows promise as a natural insect-derived antioxidant material, capable of suppressing oxidative stress by showing a stronger antioxidant effect under H2O2 stimulation than the other fractions.

Keywords

Acknowledgement

본 논문은 2024년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(NO. RS-2024-00336513)을 받아 수행된 결과의 일부로, 이에 깊은 감사를 드립니다.

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