Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Hypoglycemic and hypolipidemic effects of unsaponifiable matter from okra seed in diabetic rats

  • Dongyeon Seo (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Naeun Kim (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Ahyeong Jeon (Department of Food Science and Biotechnology, Kyungsung University) ;
  • Jihyun Kwon (Department of Food Science and Biotechnology, Kyungsung University) ;
  • In-hwan Baek (College of Pharmacy, Kyungsung University) ;
  • Eui-Cheol Shin (Department of GreenBio Science/Food Science and Technology, Gyeongsang National University) ;
  • Junsoo Lee (Department of Food Science and Biotechnology, Chungbuk National University) ;
  • Younghwa Kim (Department of Food Science and Biotechnology, Kyungsung University)
  • Received : 2024.01.17
  • Accepted : 2024.04.04
  • Published : 2024.06.01
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Abstract

BACKGROUND/OBJECTIVES: Okra seed is a rich source of various nutritional and bioactive constituents, but its mechanism of action is still unclear. The aim of this study was to evaluated the effects on glucose uptake and serum lipid profiles of unsaponifiable matter (USM) from okra seed in adipocytes and diabetic animal models. MATERIALS/METHODS: USM was prepared from okra seed powder by saponification. The contents of phytosterols and vitamin E in USM were measured. 3T3-L1 preadipocytes were cultured for 6 days with different concentrations of USM (0-200 ㎍/mL). The diabetic rats were administered with or without USM for 5 wk. RESULTS: In the USM, the contents of phytosterols and vitamin E were 394.13 mg/g USM and 31.16 mg/g USM, respectively. USM showed no cytotoxicity and led to an approximately 1.4-fold increase in glucose uptake in 3T3-L1 adipocytes. The treatment of USM also increased the expressions of peroxisome proliferator-activated receptor-γ and glucose transporter-4 in a dose-dependent manner in adipocytes. The body weight change was not significantly different in all diabetic rats. However, blood glucose and the weights of liver and adipose tissues were significantly reduced compared to those in the control diabetic rats. Treatment with USM decreased the levels of triglycerides, total cholesterol, and low-density lipoprotein cholesterol compared to the control group. The USM group also showed significantly decreased atherogenic indices and cardiac risk factors. CONCLUSION: These results suggest that USM from okra seed improves the hypoglycemic and hypolipidemic effects in diabetic rats, and provides valuable information for improving the functional properties of okra seed.

Keywords

Acknowledgement

This research was supported by National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. 2017R1C1B1008236). Additionally, it received support from the BB21plus funded by Busan Metropolitan City and Busan Techno Park.

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