Nutrition Research and Practice

The Korean Nutrition Society (한국영양학회)
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Anti-osteoporotic effects of Boswellia serrata gum resin extract in vitro and in vivo

  • Hyun Sook Lee (Department of Food Science & Nutrition, Dongseo University) ;
  • Jae In Jung (Industry coupled Cooperation Center for Bio Healthcare Materials, Hallym University) ;
  • In-Kee Hong (Health Functional Food Material Development Team, Bio Lab., Frombio Co., Ltd.) ;
  • YoungSun Jang (Health Functional Food Material Development Team, Bio Lab., Frombio Co., Ltd.) ;
  • Hye-Bin Kim (Health Functional Food Material Development Team, Bio Lab., Frombio Co., Ltd.) ;
  • Eun Ji Kim (Industry coupled Cooperation Center for Bio Healthcare Materials, Hallym University)
  • Received : 2024.01.29
  • Accepted : 2024.04.08
  • Published : 2024.06.01
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Abstract

BACKGROUND/OBJECTIVES: This study evaluated the beneficial effects of an ethanol extract of Boswellia serrata gum resin (FJH-UBS) in osteoporosis. MATERIALS/METHODS: MC3T3-E1 osteoblastic cells and RAW 264.7 osteoclastic cells were treated with FJH-UBS. The alkaline phosphatase (ALP) activity, mineralization, collagen synthesis, osteocalcin content, and Runt-related transcription factor 2 (RUNX2) and Osterix expression were measured in MC3T3-E1 cells. The actin ring structures, tartrate-resistant acid phosphatase (TRAP) activity, and the nuclear factor of activator T-cells, cytoplasm 1 (NFATc1) expression were evaluated in RAW 264.7 cells. Ovariectomized ICR mice were orally administered FJH-UBS for eight weeks. The bone mineral density (BMD) and the serum levels of osteocalcin, procollagen 1 N-terminal propeptide (P1NP), osteoprotegerin, and TRAP 5b were analyzed. RESULTS: FJH-UBS increased the ALP activity, collagen, osteocalcin, mineralization, and RUNX2 and osterix expression in MC3T3-E1 osteoblastic cells, whereas it decreased the TRAP activity, actin ring structures, and NFATc1 expression in RAW 264.7 osteoclastic cells. In ovariectomy-induced osteoporosis mice, FJH-UBS positively restored all of the changes in the bone metabolism biomarkers (BMD, osteocalcin, P1NP, osteoprotegerin, and TRAP 5b) caused by the ovariectomy. CONCLUSION: FJH-UBS has anti-osteoporotic activity by promoting osteoblast activity and inhibiting osteoclast activity in vitro and in vivo, suggesting that FJH-UBS is a potential functional food ingredient for osteoporosis.

Keywords

Acknowledgement

The authors thank the Frombio Co., Ltd. for providing Boswellia serrata gum resin extract (FJH-UBS).

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