The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Rhus verniciflua Stokes Extract on Apoptosis and Autophagy in human leukemic cell line (MOLT-4)

칠피(漆皮)가 급성림프구성백혈병 세포주 MOLT-4의 세포자멸과 자가포식에 미치는 영향

  • Juyoung Lee (Dept. of Internal Medicine, College of Korean Medicine, Kyung Hee University) ;
  • Jang-Hoon Lee (Dept. of Internal Medicine, College of Korean Medicine, Kyung Hee University) ;
  • Youngchul Kim (Dept. of Internal Medicine, College of Korean Medicine, Kyung Hee University)
  • 이주영 (경희대학교 한의과대학 간계내과학교실) ;
  • 이장훈 (경희대학교 한의과대학 간계내과학교실) ;
  • 김영철 (경희대학교 한의과대학 간계내과학교실)
  • Received : 2024.07.24
  • Accepted : 2024.08.19
  • Published : 2024.09.01
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Abstract

Objectives: This study was performed to investigate the effects of Rhus verniciflua Stokes (RVS) on apoptosis and autophagy in the human leukemic cell line, MOLT-4. Methods: Cell viability was measured by MTS/PMS assay, and cell cycle distribution was analyzed by flow cytometry. The expression levels of mRNA implicated in apoptosis and ER-stress were investigated with RT-qPCR. Lastly, apoptosis- and autophagy-related protein expressions were measured by Western blot analysis. Results: RVS inhibited proliferation of MOLT-4 in a dose-dependent manner over 24, 48 and 72 hours. RVS treatment also induced an increase in subG1 phase. Exposure to RVS increased the expression of the mRNAs encoding Bax and caspase-3, while decreasing the expression of Bcl-2 mRNA, suggesting that RVS induced apoptosis in MOLT-4 cells. Additionally, RVS extract up-regulated ER-stress related mRNAs such as IRE1α, CHOP, PERK and ATF6. Changes in RVS extract-induced apoptosis and autophagy proteins on MOLT-4 cells were also investigated. The level of Bcl-2 was decreased, whereas the levels of Bax, caspase-3, AMPK, Beclin-1, Atg5, p62, and LC3II were increased. Conclusion: These results suggest that RVS would be beneficial in the treatment of Acute Lymphoblastic Leukemia.

Keywords

References

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