The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Prediction of Treatment Mechanisms of Scutellariae Radix on Viral Pneumonia Through Network Pharmacology: Focus on Hypoxic State Regulation Through HIF-1α and HSP90

네트워크 약리학 분석을 통한 황금의 바이러스성 폐렴 치료 기전 예측: HIF-1α와 HSP90 조절을 통한 저산소 상태 조절을 중심으로

  • Jee-won Shon (Department of Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Do Kyung Han (Department of Korean Medicine, School of Korean Medicine, Pusan National University) ;
  • Youn Sook Kim (Research Institute for Longevity and Well-Being, Pusan National University) ;
  • Won Gun An (Department of Korean Medicine, School of Korean Medicine, Pusan National University)
  • 손지원 (부산대학교 한의학전문대학원 한의학과) ;
  • 한도경 (부산대학교 한의학전문대학원 한의학과) ;
  • 김윤숙 (부산대학교 장수웰빙연구소) ;
  • 안원근 (부산대학교 한의학전문대학원 한의학과)
  • Received : 2024.04.15
  • Accepted : 2024.05.14
  • Published : 2024.06.01
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Abstract

Objectives: In this study, we used network-based systems pharmacology analysis and molecular docking methods to predict the therapeutic mechanism of Scutellariae Radix on viral pneumonia. Methods: We screened active components of Scutellariae Radix and its' genes by TCMSP. Also, we extracted viral pneumonia related target genes through Gene Cards, CTD and DisGeNet. To construct Protein-protein Interaction, STRING database was used. For functional enrichment, using SRplot platform, genes were classified by 3 categories: cellular component (CC), molecular function (MF) and biological process (BP). Molecular docking was conducted by AutoDockTools (version 4.2.6). Results: 32 Network-based systematic pharmacology analysis identified 37 target genes associated with baicalein. Based on the network and gene ontology analysis of the active ingredient's target genes and disease target genes, we identified nine core genes (AKT1, BAX, BCL2, CASP3, HIF1A, PTGS2, RELA, TP53, VEGFA) and HSP90 as involved. Notably, HIF1A showed the highest relevance, overlapping with two or more utilized programs. Hypoxia-inducible factor 1-alpha (HIF-1α) has been implicated in the expression of inflammatory cytokines, the induction of hypoxia, and the triggering of cytokine storms. Baicalein, a major component of SR, binds to both HIF-1α and HSP90, suggesting that it may be a possible targeted treatment for viral pneumonia. Conclusions: Baicalein may bind to HIF-1α to control inflammation caused by viral infectious diseases and may also regulate hypoxic conditions to prevent impairment of lung function caused by an overactive immune system. These findings suggest further research into the molecular mechanisms involved in hypoxia and provide a scientific basis for improving the treatment of viral infectious diseases.

Keywords

Acknowledgement

본 연구는 한국연구재단 [NRF-2021R1I1A3A04037158], [NRF-2021R1I1A1A01058697] 지원을 받아 수행되었음.

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