한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제37권5호
  • /
  • Pages.282-291
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  • 2024
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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황기 조다당 획분 유래 효소 가수분해물의 면역 자극 활성

Immunostimulatory Activity of Enzymatic Hydrolysates Derived from Crude Polysaccharides of Astragalus membranaceus

  • 정원비 (국립한국교통대학교 식품영양학전공) ;
  • 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) ;
  • 김효원 (국립한국교통대학교 식품영양학전공) ;
  • 김다은 (국립한국교통대학교 식품영양학전공) ;
  • 신지영 ((주)코시스바이오) ;
  • 신광순 (경기대학교 식품생물공학과) ;
  • 유광원 (국립한국교통대학교 식품영양학전공)
  • Won Bi Jeong (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Hyun Young Shin (Transdisciplinary Major in Learning Health Systems, Dept. of Integrated Biomedical and Life Science, Graduate School, Korea University) ;
  • Hyo Won Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Da Eun Kim (Major in Food and Nutrition, Korea National University of Transportation) ;
  • Ji-Young Shin (Cosis-Bio Co., Ltd.) ;
  • Kwang-Soon Shin (Dept. of Food Science and Biotechnology, Kyonggi University) ;
  • Kwang-Won Yu (Major in Food and Nutrition, Korea National University of Transportation)
  • 투고 : 2024.09.25
  • 심사 : 2024.10.16
  • 발행 : 2024.10.31
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초록

In a previous study, we fractionated crude polysaccharide (AME-CP) with macrophage-stimulating activity from a hot-water extract (AME) of Astragalus membranaceus. AME-CP contained glucose (Glc) as a main component sugar, suggesting that it might be rich in starch-like compounds (SLC). To enhance the immunostimulating activity of AME-CP by pruning SLC rarely known to contribute to activity, hydrolysate (AME-SH) was prepared by digesting with starch-related enzymes, including α-amylase and amyloglucosidase. AME-SH was found to contain substances with molecular weights ranging from 3.9 to 84.4 kDa. These substances were primarily composed of galactose, galacturonic acid, Glc, arabinose, rhamnose, and mannose. AME-SH significantly enhanced the production of macrophage-stimulating factors, including nitric oxide (NO), interleukin (IL)-6, and IL-12, in RAW 264.7 cells compared to AME-CP. Treatment of splenocytes isolated from C3H/HeN mice with AME-SH not only promoted IL-6 secretion, but also induced mitogenic activity. In addition, AME-SH promoted the secretion of hematopoietic growth factors including IL-6 and granulocyte-macrophage colony-stimulating factor (GM-CSF) in Peyer's patch (PP) cells and stimulated bone marrow cell proliferation through these PP cells. In conclusion, hydrolysate (AME-SH) digested from AME-CP with starch-related enzymes could be used as a potential immunostimulant.

키워드

과제정보

본 논문은 2024년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과입니다(2021RIS-001; 프로젝트랩).

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