The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Protective Effects of Sinomenium acutum Rhizome and Its Alkaloids against Dexamethasone-induced Atrophy in C2C12 Myoblasts

방기 뿌리 (Sinomenium acutum rhizome) 추출물 및 그 알칼로이드 성분의 항근위축 효과

  • Kyong Kim (Dept. of Food and Nutrition, Eulji University) ;
  • Yoon Sin Oh (Dept. of Food and Nutrition, Eulji University)
  • 김경 (을지대학교 식품영양학과) ;
  • 오윤신 (을지대학교 식품영양학과)
  • Received : 2024.08.30
  • Accepted : 2024.10.16
  • Published : 2024.10.31
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Abstract

Skeletal muscle is an organ that regulates biological metabolic energy. Its dysfunction causes decline of body functions and disability, thus deteriorating the overall quality of life. Various materials are being developed with an anti-sarcolytic effect. However, anti-sarcolytic effect of Sinomenium acutum rhizomes extract (SAE) remains unclear. Therefore, this study aimed to investigate anti-muscle atrophy effects of SAE and its alkaloids, including sinomenine (SIN), magnoflorine (MF), acutumine (ACU), and N-ferultyramine (NFT) isolated from SAE, on dexamethasone (Dex)-induced myotubules. C2C12 myogenic cells differentiated for 6 days were treated with 1 mM Dex for 24 hours. Induction of muscular atrophy was confirmed by a decrease in myogenin expression. We found that Dex increased expression levels of muscle-specific ubiquitin ligases MuRF1 and MAFbx/atrogin-1. However, mRNA and protein levels of these muscle-specific ubiquitin ligases were significantly reduced by cotreatment with SIN, MF, and NFT in myotubes. Glucose uptake reduced by Dex in myotubules were also restored by SIN, MF, and NFT treatments. These results suggest that SIN, MF, and NFT can reduce muscle wasting and enhance glucose uptake in Dex-treated myotubes, highlighting their potential as therapeutic agents to prevent muscle atrophy.

Keywords

Acknowledgement

본 논문은 2024년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원(NO. RS-2024-00336513)을 받아 수행된 결과의 일부로, 이에 깊은 감사를 드립니다.

References

  1. Bae KH, Min BS, Do DS, Kim NS, Yang GJ, Ahn BZ. 1992. Screening on cytotoxicity of medicinal plants against L1210 cell. Yakhak Hoeji 36:491-495
  2. Bentzinger CF, Wang YX, Rudnicki MA. 2012. Building muscle: Molecular regulation of myogenesis. Cold Spring Harb Perspect Biol 4:a008342
  3. Bodine SC, Baehr LM. 2014. Skeletal muscle atrophy and the E3 ubiquitin ligases MuRF1 and MAFbx/atrogin-1. Am J Physiol Endocrinol Metabol 307:E469-E484
  4. Bodine SC, Latres E, Baumhueter S, Lai VKM, Nunez L, Clarke BA, Poueymirou WT, Panaro FJ, Na E, Dharmarajan K, Pan ZQ, Valenzuela DM, DeChiara TM, Stitt TN, Yancopoulos GD, Glass DJ. 2001. Identification of ubiquitin ligases required for skeletal muscle atrophy Science 294:1704-1708
  5. Bonaldo P, Sandri M. 2013. Cellular and molecular mechanisms of muscle atrophy. Dis Model Mech 6:25-39
  6. Chen J, Guo P, Han M, Chen K, Qin J, Yang F. 2023. Cognitive protection of sinomenine in type 2 diabetes mellitus through regulating the EGF/Nrf2/HO-1 signaling, the microbiota-gut-brain axis, and hippocampal neuron ferroptosis. Phytother Res 37:3323-3341
  7. Clarke BA, Drujan D, Willis MS, Murphy LO, Corpina RA, Burova E, Rakhilin SV, Stitt TN, Patterson C, Latres E, Glass DJ. 2007. The E3 Ligase MuRF1 degrades myosin heavy chain protein in dexamethasone-treated skeletal muscle. Cell Metab 6:376-385
  8. Cohen S, Brault JJ, Gygi SP, Glass DJ, Valenzuela DM, Gartner C, Latres E, Goldberg AL. 2009. During muscle atrophy, thick, but not thin, filament components are degraded by MuRF1-dependent ubiquitylation. J Cell Biol 185:1083-1095
  9. Csibi A, Cornille K, Leibovitch MP, Poupon A, Tintignac LA, Sanchez AMJ, Leibovitch SA. 2010. The translation regulatory subunit eIF3f controls the kinase-dependent mTOR signaling required for muscle differentiation and hypertrophy in mouse. PLOS ONE 5:e8994
  10. DeFronzo RA. 2009. Banting Lecture. From the triumvirate to the ominous octet: A new paradigm for the treatment of type 2 diabetes mellitus. Diabetes 58:773-795
  11. Faulkner JA, Larkin LM, Claflin DR, Brooks SV. 2007. Age-related changes in the structure and function of skeletal muscles. Clin Exp Pharmacol Physiol 34:1091-1096
  12. Ferri P, Barbieri E, Burattini S, Guescini M, D'Emilio A, Biagiotti L, Del Grande P, De Luca A, Stocchi V, Falcieri E. 2009. Expression and subcellular localization of myogenic regulatory factors during the differentiation of skeletal muscle C2C12 myoblasts. J Cell Biochem108: 1302-1317
  13. Fielding RA, Vellas B, Evans WJ, Bhasin S, Morley JE, Newman AB, Abellan van Kan G, Andrieu S, Bauer J, Breuille D, Cederholm T, Chandler J, De Meynard C, Donini L, Harris T, Kannt A, Guibert FK, Onder G, Papanicolaou D, Rolland Y, Rooks D, Sieber C, Souhami E, Verlaan S, Zamboni M. 2011. Sarcopenia: An undiagnosed condition in older adults. Current consensus definition: Prevalence, etiology, and consequences. International working group on sarcopenia. J Am Med Dir Assoc 12:249-256
  14. Ghee SS, Lee YJ. 2005.The therapeutic effects of bangkeehwangkee-tang and bangkeebokryeong-tang on the hyperlipidemia in rats. Korean J Herbology 20:149-157
  15. Gomes MD, Lecker SH, Jagoe RT, Navon A, Goldberg AL. 2001. Atrogin-1, a muscle-specific F-box protein highly expressed during muscle atrophy. Proc Natl Acad Sci USA 98:14440-14445
  16. Gwag T, Park K, Kim E, Son C, Park J, Nikawa T, Choi I. 2013. Inhibition of C2C12 myotube atrophy by a novel HSP70 inducer, celastrol, via activation of Akt1 and ERK1/2 pathways. Arch Biochem Biophys 537:21-30
  17. Hasselgren PO. 1999. Glucocorticoids and muscle catabolism. Curr Opin Clin Nutr Metab Care 2:201-205
  18. Hasselgren PO, Menconi MJ, Fareed MU, Yang H, Wei W, Evenson A. 2005. Novel aspects on the regulation of muscle wasting in sepsis. Int J Biochem Cell Biol 37:2156-2168
  19. Hasty P, Bradley A, Morris JH, Edmondson DG, Venuti JM, Olson EN, Klein WH. 1993. Muscle deficiency and neonatal death in mice with a targeted mutation in the myogenin gene. Nature 364:501-506
  20. He Y, Yang P, Yuan T, Zhang L, Yang G, Jin J, Yu T. 2023. miR-103-3p regulates the proliferation and differentiation of C2C12 myoblasts by targeting BTG2. Int J Mol Sci 24:15318
  21. Jeong H, Lee JY, Jang EJ, Lee EH, Bae MA, Hong JH, Hwang ES. 2011. Hesperedin promotes MyoD-induced myogenic differentiation in vitro and in vivo. Br J Pharmacol 163:598-608
  22. Jeong JS, Park CH, Kim, IB, Kim, JB. 2017. Activation of signaling pathways for protein synthesis by Korean mistletoe (Viscum album coloratum) extract in a mouse model of muscle atrophy. Korean J Food Nutr 30:371-377
  23. Jiang R, Wang M, Shi L, Zhou J, Ma R, Feng K, Chen X, Xu X, Li X, Li T, Sun L. 2019. Panax ginseng total protein facilitates recovery from dexamethasone-induced muscle atrophy through the activation of glucose consumption in C2C12 myotubes. BioMed Res Int 2019:3719643
  24. Jung EJ, Kim JH, Kim HM, Guo S, Lee DH, Lim GM, Syed ASh, Kim W, Kim CY. 2023. Isolation of alkaloids from Sinomenium acutum by centrifugal partition chromatography and their ameliorating effects on dexamethasone-induced atrophy in C2C12 myotubes. Separations 10:470
  25. Kim HM, Moon PD, Chae HJ, Kim HR, Chung JG, Kim JJ, Lee EJ. 2000. The stem of Sinomenium acutum inhibits mast cell-mediated anaphylactic reactions and tumor necrosis factor-α production from rat peritoneal mast cells. J Ethnopharmacol 70:135-141
  26. Kim TH, Kim YI, Hong KE, Yim YK, Lee H, Lee BR. 2004. Influence on the anti-cancer and immune response improvement of herbal-acupuncture with Sinomenii acuti Lignum infusion solution into joksamni (ST36). Korean J Meridian Acupoint 21:79-93
  27. Kuo T, Harris CA, Wang JC. 2013. Metabolic functions of glucocorticoid receptor in skeletal muscle. Mol Cell Endocrinol 380:79-88
  28. Kurth-Kraczek EJ, Hirshman MF, Goodyear LJ, Winder WW. 1999. 5' AMP-activated protein kinase activation causes GLUT4 translocation in skeletal muscle. Diabetes 48:1667-1671
  29. Lee H. 2004. Influence on the Anti-cancer and immune response improvement of Herbal-acupuncture with Sinomenii acuti Lignum infusion solution into chung-wan (CV12). J Acupunct Res 21:85-102
  30. Lee J, Weon JB, Yun BR, Eom MR, Ma CJ. 2013. Quantitative analysis for the quality evaluation of sinomenine, magnoflorine and syringaresinol in Sinomenium acutum. Yakhak Hoeji 57:161-166
  31. Liu L, Buchner E, Beitze D, Schmidt-Weber CB, Kaever V, Emmrich F, Kinne RW. 1996. Amelioration of rat experimental arthritides by treatment with the alkaloid sinomenine. Int J Immunopharmacol 18:529-543
  32. Mokdad AH, Bowman BA, Ford ES, Vinicor F, Marks JS, Koplan JP. 2001. The continuing epidemics of obesity and diabetes in the United States. JAMA 286:1195-1200
  33. Ochi A, Abe T, Nakao R, Yamamoto Y, Kitahata K, Takagi M, Hirasaka K, Ohno A, Teshima-Kondo S, Gwag T, Choi I, Kawamura T, Nemoto H, Mukai R, Terao J, Nikawa T. 2015. N-myristoylated ubiquitin ligase Cbl-b inhibitor prevents on glucocorticoid-induced atrophy in mouse skeletal muscle. Arch Biochem Biophys 570:23-31
  34. Park HC, Jeong JW, Choi YH. 2017. Induction of muscle atrophy by dexamethasone and hydrogen peroxide in differentiated C2C12 myotubes. J Life Sci 27:1479-1485
  35. Richter EA, Hargreaves M. 2013. Exercise, GLUT4, and skeletal muscle glucose uptake. Physiol Rev 93:993-1017
  36. Rosenberg IH. 1997. Sarcopenia: origins and clinical relevance. J Nutr 127:990S-991S
  37. Sartori R, Romanello V, Sandri M. 2021. Mechanisms of muscle atrophy and hypertrophy: Implications in health and disease. Nat Commun 12:330
  38. Won HS. 1991. Age-related changes in body growth, muscle protein content and cellular-mediated immunity and rats fed stock diets. Korean J Food and Nutr 4:133-139
  39. Xu M, Liu S, Wan R, Chen Y. 2018. Combined treatment with sinomenine and acupuncture on collagen induced arthritis through the NF‑κB and MAPK signaling pathway. Oncol Lett 15:8770-8776
  40. Yadav A, Singh A, Phogat J, Dahuja A, Dabur R. 2021. Magnoflorine prevent the skeletal muscle atrophy via Akt/mTOR/FoxO signal pathway and increase slow-MyHC production in streptozotocin-induced diabetic rats. J Ethnopharmacol 267:113510
  41. Yamasaki H. 1976. Pharmacology of sinomenine, an anti-rheumatic alkaloid from Sinomenium acutum. Acta Med Okayama 30:1-20
  42. Zhang B, Zhang H, Luo H, Yang C, Yuan Y. 2019. Sinomenine can promote the proliferation and differentiation of osteoblasts by regulating the Akt/Runx2 signaling pathway in MC3T3-E1 cells. Pharmazie 74:747-750
  43. Zhao ZZ, Liang ZT, Zhou H, Jiang ZH, Liu ZQ, Wong YF, Xu HX, Liu L. 2005. Quantification of sinomenine in Caulis sinomenii collected from different growing regions and wholesale herbal markets by a modified HPLC method. Biol Pharm Bull 28:105-109