The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Effects of Rabbit Meat Extract on Myogenic Differentiation and Energy Regulation in C2C12 Cells

C2C12 세포에서 토끼고기 추출물의 근육분화 및 에너지 조절 효과

  • In-Seon Bae (Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jeong Ah Lee (Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration) ;
  • Won-Seo Park (Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jayeon Yoo (Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration) ;
  • Jun-Sang Ham (Animal Products Utilization Division, National Institute of Animal Science, Rural Development Administration) ;
  • Kangmin Seo (Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration) ;
  • Ki Hyun Kim (Animal Welfare Research Team, National Institute of Animal Science, Rural Development Administration)
  • 배인선 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 이정아 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 박원서 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 유자연 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 함준상 (농촌진흥청 국립축산과학원 축산물이용과) ;
  • 서강민 (농촌진흥청 국립축산과학원 동물복지연구팀) ;
  • 김기현 (농촌진흥청 국립축산과학원 동물복지연구팀)
  • Received : 2024.08.28
  • Accepted : 2024.10.07
  • Published : 2024.10.31
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Abstract

The present study was conducted to investigate effects of rabbit meat extract on energy metabolism and muscle differentiation in C2C12 myotubes. Water extract of rabbit meat (10, 50, 100, and 200 ㎍/ml) was used to treat differentiated C2C12 cells. Reverse transcriptase polymerase chain reaction (RT-PCR) and western blot analysis were used to determine mRNA or protein levels of energy metabolism-related genes. Total adenosine triphosphate (ATP) content was also measured. Treatment with rabbit meat extract significantly increased expression levels of muscle differentiation markers (myogenin and myosin heavy chain) and mitochondrial biogenesis regulators (PGC1α, NRF1, and TFAM) in C2C12 myotubes compared to non-treated control. Additionally, rabbit meat extract activated phosphorylation of AMPK and acetyl-coA carboxylase (ACC). Rabbit meat extract significantly increased ATP contents in myotubes. These results suggest that rabbit meat extract has the potential to improve energy metabolism in skeletal muscles.

Keywords

Acknowledgement

본 연구는 농촌진흥청 연구사업(과제번호:RS-2022-RD010371)의 지원에 의해 이루어진 것임.

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