Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Catechin reduces liver inflammation by regulating Kupffer cell activation in rats

  • Meejung Ahn (Department of Animal Science, College of Life Science, Sangji University)
  • Received : 2024.08.05
  • Accepted : 2024.09.04
  • Published : 2024.09.30
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Abstract

The liver is a defense against infections due to its strategic location between the gastrointestinal and systemic circulations. In dogs and cats, infectious hepatitis encompasses a range of contagious diseases affecting the liver either directly or as part of a broader systemic infection, including bacterial, mycobacterial, viral, fungal, protozoal, parasitic, and rickettsial diseases. Catechins possess well-recognized natural antioxidant properties. This study investigated their therapeutic potential for applications in hepatology, evaluating whether catechins reduce hepatic inflammation in rats repeatedly exposed to carbon tetrachloride (CCl4). Sprague-Dawley rats were given catechin 50 (C50) or 100 (C100) mg/kg body weight orally daily for 3 days. This treatment was given with or without concurrent intraperitoneal injections of CCl4. Phosphate-buffered saline served as the vehicle control, while silymarin administered at 100 mg/kg was used as the positive control. Gross examination revealed significant enlargement, edema, and darker tissue in CCl4-induced livers treated with vehicle. Additionally, spotty discoloration was observed on the surface. Kupffer cell activation suppressed the expression of inflammatory mediators, including inducible nitric oxide synthase (iNOS), in groups co-treated with catechin and CCl4; this effect was reversed when catalase replaced catechin in CCl4-injured rats. Catechin alleviates hepatic inflammation in rats repeatedly exposed to CCl4; it also modulates the activation of Kupffer cells and monocytes. These results should lead to new treatments for liver inflammation in veterinary practice.

Keywords

Acknowledgement

This research was supported by the National Research Foundation of Korea (grant number: NRF-2021M3H9A1097596), and the 2022 scientific promotion program funded by Sangji University.

References

  1. Kearns S. Infectious hepatopathies in dogs and cats. Top Companion Anim Med. 2009;24:189-198.
  2. Howe LM, Boothe DM, Boothe HW. Detection of portal and systemic bacteremia in dogs with severe induced hepatic disease and multiple portosystemic shunts. Am J Vet Res. 1999;60:181-185.
  3. Edwards MJ, Keller BJ, Kauffman FC, Thurman RG. The involvement of Kupffer cells in carbon tetrachloride toxicity. Toxicol Appl Pharmacol. 1993;119:275-279.
  4. Ahn M, Kim J, Bang H, Moon J, Kim GO, Shin T. Hepatoprotective effects of allyl isothiocyanate against carbon tetrachloride-induced hepatotoxicity in rat. Chem Biol Interact. 2016;254:102-108.
  5. Ahn M, Park JS, Chae S, Kim S, Moon C, Hyun JW, et al. Hepatoprotective effects of Lycium chinense Miller fruit and its constituent betaine in CCl4-induced hepatic damage in rats. Acta Histochem. 2014;116:1104-1112.
  6. Abdel-Hamid NM, Faddah LM, Al-Rehany MA, Ali AH, Bakeet AA. New role of antinutritional factors, phytic acid and catechin in the treatment of CCl4 intoxication. Ann Hepatol. 2007;6:262-266.
  7. Bernatoniene J, Kopustinskiene DM. The role of catechins in cellular responses to oxidative stress. Molecules. 2018;23:965.
  8. Jullian C, Miranda S, Zapata-Torres G, Mendizabal F, OleaAzar C. Studies of inclusion complexes of natural and modified cyclodextrin with (+)catechin by NMR and molecular modeling. Bioorg Med Chem. 2007;15:3217-3224.
  9. Liu J, Lu JF, Wen XY, Kan J, Jin CH. Antioxidant and protective effect of inulin and catechin grafted inulin against CCl4-induced liver injury. Int J Biol Macromol. 2015;72:1479-1484.
  10. Yeh MM, Brunt EM. Pathological features of fatty liver disease. Gastroenterology. 2014;147:754-764.
  11. Dani C, Bonatto D, Salvador M, Pereira MD, Henriques JA, Eleutherio E. Antioxidant protection of resveratrol and catechin in Saccharomyces cerevisiae. J Agric Food Chem. 2008;56:4268-4272.
  12. Chen M, Wang T, Yue F, Li X, Wang P, Li Y, et al. Tea polyphenols alleviate motor impairments, dopaminergic neuronal injury, and cerebral α-synuclein aggregation in MPTP-intoxicated parkinsonian monkeys. Neuroscience. 2015;286:383-392.
  13. Farkhondeh T, Yazdi HS, Samarghandian S. The protective effects of green tea catechins in the management of neurodegenerative diseases: a review. Curr Drug Discov Technol. 2019;16:57-65.
  14. Musial C, Kuban-Jankowska A, Gorska-Ponikowska M. Beneficial properties of green tea catechins. Int J Mol Sci. 2020;21:1744.
  15. Kawashima R, Mochida S, Matsui A, YouLuTuZ Y, Ishikawa K, Toshima K, et al. Expression of osteopontin in Kupffer cells and hepatic macrophages and Stellate cells in rat liver after carbon tetrachloride intoxication: a possible factor for macrophage migration into hepatic necrotic areas. Biochem Biophys Res Commun. 1999;256:527-531.
  16. Pervin M, Golbar HM, Bondoc A, Izawa T, Kuwamura M, Yamate J. Immunophenotypical characterization and influence on liver homeostasis of depleting and repopulating hepatic macrophages in rats injected with clodronate. Exp Toxicol Pathol. 2016;68:113-124.
  17. Li J. Neuroprotective effect of (-)-epigallocatechin-3-gallate on autoimmune thyroiditis in a rat model by an anti-inflammation effect, anti-apoptosis and inhibition of TRAIL signaling pathway. Exp Ther Med. 2018;15:1087-1092.
  18. Yang N, Ko M, Ahn M, Shin T. Hepatoprotective effects of norgalanthamine on carbon tetrachloride induced-hepato-toxicity in mice. Drug Chem Toxicol. 2023;46:144-154.