Journal of Veterinary Science

  • 제25권5호
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  • Pages.67.1-67.8
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  • 2024
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Prevalence and molecular characteristics of carbapenem-resistant Escherichia coli isolated from dogs in South Korea

  • Bo-Youn Moon (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Md. Sekendar Ali (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Seunghoe Kim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Hee-Seung Kang (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Ye-Ji Kang (Division of Health Hazard Response, Korea Disease Control and Prevention Agency) ;
  • Jae-Myung Kim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Dong-Chan Moon (Centre for Infectious Diseases Research, Korea Centers for Disease Control and Prevention) ;
  • Suk-Kyung Lim (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • 투고 : 2024.06.17
  • 심사 : 2024.08.13
  • 발행 : 2024.09.30
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초록

Importance: Carbapenem-resistant Enterobacteriaceae are emerging as a global public health risk. Therefore, assessing the prevalence of carbapenem-resistant Escherichia coli (CRE) in both humans and animals is important. Objective: We aimed to ascertain the occurrence and characteristics of CRE isolated from companion animals, dogs and cats. Methods: E. coli strains were tested for antimicrobial susceptibility using the broth microdilution technique. Antimicrobial resistance genes were detected by polymerase chain reaction and sequencing analysis. The molecular characteristics of CRE were determined using multi-locus sequence typing, replicon typing, and pulsed-field gel electrophoresis (PFGE). Results: In total, 13 CRE isolates (0.13%) were identified from dogs possessing blaNDM-5 along with β-lactamase genes, mostly blaCMY-2 (92.2%) and blaTEM-1 (53.8%). The commonly observed mutations were S83L and D87N in gyrA, S80I in parC, and S458A in parE. CRE carried non-beta-lactam resistance genes, with the majority being tet(B) (100%), sul (84.6%), and aac(3)-II (53.8%). Nine different PFGE patterns (P1-P9), IncX3-type plasmids (69.2%), and ST410 (84.6%) were predominantly detected. Conclusions and Relevance: This investigation provides significant insight into the prevalence and molecular characteristics of blaNDM-5-carrying E. coli in dogs. The co-existence of blaNDM-5 and other antimicrobial resistance genes in E. coli potentially poses severe health hazards to humans.

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과제정보

This study was supported by the Animal and Plant Quarantine Agency, Ministry of Agriculture, Food, and Rural Affairs, Republic of Korea (grant number: B-1543081-2024-26-01).

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