Journal of Veterinary Science

  • 제25권5호
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  • Pages.61.1-61.13
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  • 2024
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  • 1229-845X(pISSN)
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  • 1976-555X(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
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Identification of concurrent infection with Jaagsiekte sheep retrovirus and maedi-visna virus in China

  • Xujie Duan (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Xiaona Shi (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Pei Zhang (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Xiaoyue Du (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Sixu Chen (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Liang Zhang (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Huiping Li (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Yufei Zhang (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Jinling Wang (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Yulin Ding (College of Veterinary Medicine, Inner Mongolia Agricultural University) ;
  • Shuying Liu (College of Veterinary Medicine, Inner Mongolia Agricultural University)
  • 투고 : 2024.06.19
  • 심사 : 2024.07.14
  • 발행 : 2024.09.30
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초록

Importance: Ovine pulmonary adenomatosis (OPA) and maedi-visna disease (MVD) are chronic and progressive infectious diseases in sheep caused by Jaagsiekte sheep retrovirus (JSRV) and maedi-visna virus (MVV), respectively. Objective: To investigate the pathological changes and conduct viral gene analysis of OPA and MVD co-occurrence in Inner Mongolia, China. Methods: Using gross pathology, histopathology, immunohistochemistry, ultrastructural pathology, PCR, and sequence analysis, we investigated the concurrent infection of JSRV and MVV in 319 Dorper rams slaughtered in a private slaughterhouse in Inner Mongolia, in 2022. Results: Of the 319 rams included, 3 showed concurrent JSRV and MVV infection. Gross lung pathology showed diffuse enlargement, consolidation, and greyish-white miliary nodules on the lung surface; the trachea was filled with a white foamy fluid; hilar and mediastinal lymph nodes were significantly enlarged. Histopathology results revealed typical OPA and MVD lesions in the lung tissue. Immunohistochemical results were positive for JSRV envelope protein (Env) in the tumor cells and MVV CA in alveolar macrophages. Transmission electron microscopy showed several virions and autophagosomes in the lung tissue, severely damaged mitochondria, and the induced mitophagy. Nucleotide sequences obtained for JSRV env and MVV gag showed the highest homology with the Inner Mongolian strains of JSRV env (JQ837489) and MVV gag (MW248464). Conclusions and Relevance: Our study confirmed that OPA and MVD co-occurrence and identified the pathological changes in Inner Mongolia, China, thereby providing references for the identification of concurrent JSRV and MVV infections.

키워드

과제정보

We are grateful to Dr. Kai Zhang, Dr. Yuanyuan Zhang, Dr. Jiamin Zhao and Mr. Bo Feng for their help in the experiment.

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