Journal of Biomedical Engineering Research (대한의용생체공학회:의공학회지)

The Korean Society of Medical and Biological Engineering (대한의용생체공학회)
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Effects of In Vitro Vibration Loading on Myogenesis of C2C12 Myoblasts

C2C12 근아세포의 근육 형성에 대한 진동 부하 효과

  • Yong Chan Jung (Department of Biomedical Engineering, Yonsei University) ;
  • Gayoung Kim (Department of Biomedical Engineering, Yonsei University) ;
  • Eunyeong Moon (Department of Biomedical Engineering, Yonsei University) ;
  • Hanbyul Cho (Department of Biomedical Engineering, Yonsei University) ;
  • Chi Hyun Kim (Department of Biomedical Engineering, Yonsei University)
  • Received : 2024.08.07
  • Accepted : 2024.08.21
  • Published : 2024.08.31
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Abstract

Muscle disorders arise from genetic factors or other external environmental influences, leading to muscle loss and decreased strength. Muscle disease is closely related to age, and with the global progression of aging, there is increasing interest in the prevention and treatment of muscle diseases among the elderly population. Muscles release various substances during contraction and relaxation when exercising, and growth and differentiation are enhanced. Similar effects can be achieved by vibration stimulations of specific frequencies. To examine the response of muscle cells to vibration stimuli, an in vitro vibration device capable of setting various frequencies and amplitudes was developed for use with a 6-well plate. In vitro vibrational stimulation was applied to C2C12 myoblasts to quantify the growth and differentiation of myoblasts, as well as cell apoptosis. 10 Hz vibrational stimuli resulted in increases in ERK signaling and decreases in cell death. Moreover, an increase in the number of exposures to vibration promoted cellular differentiation. In conclusion, 10 Hz vibrational stimuli have the potential to increase muscle growth and differentiation and reduce apoptosis in C2C12 myoblasts.

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References

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