Journal of Animal Reproduction and Biotechnology (한국동물생명공학회지)

The Korean Society of Animal Reproduction and Biotechnology (한국동물생명공학회)
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Influence of aqueous extract of Annona muricata leaves in Tris-egg yolk extender on storage of spermatozoa from West African Dwarf goat (Capra hircus)

  • Mohamadou Adamou (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Dongmo Nguedia Arius Baulland (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Ngo Bahebeck Pierrette (Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center) ;
  • Tchoffo Herve (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Chongsi Margaret Mary Momo (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Noudjio Kezeter Claude (Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center) ;
  • Nnanga, Germaine Estelle Mvondo (Institute of Agricultural Reseach for Development, Mbalmayo Agricultural Research Center) ;
  • Ngwemetah Nathalie (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang) ;
  • Adamu Yusufa Njeiri (College of Technology, University of Bamenda) ;
  • Ngoula Ferdinand (Animal Physiology and Health Research Unit, Faculty of Agronomy and Agricultural Sciences, University of Dschang)
  • Received : 2024.07.09
  • Accepted : 2024.09.07
  • Published : 2024.09.30
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Abstract

Background: Because oxidative stress can induce decreased quality of caprine semen during the storage, there has been limitation for the use of stored semen in the assisted reproductive technologies. The present study, therefore, assesses the potential of Annona muricata (A. muricata) to reduce semen storage associated-damages. Methods: Semen was collected by electro-ejaculation from ten bucks, and extended with Tris-egg yolk (TEY) supplemented with A. muricata leaf aqueous extract (SAE) at 20 (SAE20), 40 (SAE40), and 80 (SAE80) ㎍/mL. Sperm variables including motility, motion characteristics, viability, membrane functionality, and DNA integrity were assessed at different storage periods (6, 24, 48, and 72 hr). In addition, oxidative stress indicators in the extender supplemted with SAE were also assessed for each group. Results: By adding SAE at 80 ㎍/mL in TEY, the storage of goat buck semen was improved, resulting in reduced loss of sperm motility, viability, DNA fragmentation, and membrane integrity during chilled storage at 4℃ for up to 72 hr. In addition, enrichment of TEY extender with SAE significantly (p < 0.05) reduced malondialdehyde, an indicator of oxidative stress, compared to the negative control. Conclusions: Supplementation of SAE in TEY extender can reduce buck spermatozoa liquid storage associated damages due to oxidative stress.

Keywords

References

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