Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effects of Undaria pinnatifida and Leuconostoc mesenteroides Fermented Extracts on Adipocyte Differentiation in 3T3-L1 Cells

유산균을 활용한 미역발효추출물이 3T3-L1에서 지방세포 분화에 미치는 영향

  • Min Woo Moon (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University) ;
  • Chae Hun Ra (Department of Food Science and Biotechnology, College of Engineering, Global K-Food Research Center, Hankyong National University)
  • 문민우 (한경대학교 식품생명공학과) ;
  • 라채훈 (한경대학교 식품생명공학과)
  • Received : 2024.05.03
  • Accepted : 2024.07.08
  • Published : 2024.09.28
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Abstract

This study aimed to examine the potential inhibitory effect of Undaria pinnatfida fermented by Leuconostoc mesenteroides (UFM) on preadipocyte differentiation in the 3T3-L1 cell line. Ethanol extracts from UFM were prepared and tested for cell viability, Oil Red O staining, and quantitative reverse transcription-PCR (RT-qPCR) in 3T3-L1 adipocytes. Treatment of preadipocytes with UFM at different concentrations (50, 100, and 200 ㎍/ml) found that it inhibited lipid accumulation by 66.80%, 61.59%, and 55.94%, respectively. Furthermore, RT-qPCR showed that UFM extract reduced the gene expressions of CCAAT/enhancer-binding protein α (C/EBP α), peroxisome proliferator-activated receptor γ (PPAR γ), adipocyte-specific lipid binding protein (aP2), fatty acid synthase (FAS), and ATP citrate lyase (ACL), respectively. These results suggest that UFM can be a beneficial functional ingredient to prevent obesity in the food industry.

유산균 L. mesenteroides SGL152를 이용한 미역발효 추출물로부터 3T3-L1 지방세포 분화에 미치는 영향을 살펴보기 위해 MTT assay, Oil Red O staining, RT-qPCR 실험을 진행하였다. 유산균의 미역발효 추출물(UFM)의 농도별 세포 독성 실험 결과, 200 ㎍/ml 농도 이하에서는 비세포독성으로 판단된다. 또한 3T3-L1 지방세포 분화와 동시에 추출물을 농도별로 처리하여 분화를 진행한 결과, 대조군과 비교하여 유의적으로 지방구 형성 및 지방분화를 억제하였다. 이러한 연구결과는 유산균을 이용한 해조류 발효과정 및 복합추출물에 있어서 유용한 정보를 제공할 것으로 판단된다.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No.2022R1F1A1074594).

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