Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Study on Optimization of Liquid Fermentation Medium and Antitumor Activity of the Mycelium on Phyllopora lonicerae

  • Min Liu (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University) ;
  • Lu Liu (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University) ;
  • Guoli Zhang (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University) ;
  • Guangyuan Wang (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University) ;
  • Ranran Hou (College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University) ;
  • Yinghao Zhang (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University) ;
  • Xuemei Tian (Shandong Province Key Laboratory of Applied Mycology, College of Life Sciences, Qingdao Agricultural University)
  • Received : 2024.05.08
  • Accepted : 2024.07.22
  • Published : 2024.09.28
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Abstract

Phylloporia lonicerae is an annual fungus that specifically parasitizes living Lonicera plants, offering significant potential for developing new resource food and medicine. However, wild resources and mycelium production of this fungus is limited, and its anti-tumor active ingredients and mechanisms remain unclear, hampering the development of this fungus. Thus, we optimized the fermentation medium of P. lonicerae and studied the anti-tumor activity of its mycelium. The results indicated that the optimum fermentation medium consisted of 2% sucrose, 0.2% peptone, 0.1% KH2PO4, 0.05% MgSO4·7H2O, 0.16% Lonicera japonica petals, 0.18% P fungal elicitor, and 0.21% L. japonica stem. The biomass reached 7.82 ± 0.41 g/l after 15 days of cultivation in the optimized medium, a 142% increase compared with the potato dextrose broth medium, with a 64% reduction in cultivation time. The intracellular alcohol extract had a higher inhibitory effect on A549 and Eca-109 cells than the intracellular water extract, with half-maximal inhibitory concentration values of 2.42 and 2.92 mg/ml, respectively. Graded extraction of the alcohol extract yielded petroleum ether phase, chloroform phase, ethyl acetate phase, and n-butanol phase. Among them, the petroleum ether phase exhibited a better effect than the positive control, with a half-maximal inhibitory concentration of 113.3 ㎍/ml. Flow cytometry analysis indicated that petroleum ether components could induce apoptosis of Eca-109 cells, suggesting that this extracted component can be utilized as an anticancer agent in functional foods. This study offers valuable technical support and a theoretical foundation for promoting the comprehensive development and efficient utilization of P. lonicerae.

Keywords

Acknowledgement

This work was supported by Special Foundation for Taishan Scholar of Shandong Province (tsqn202211188), the National Natural Science Foundation of China (31970014).

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