Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Roles of Endophytic Fungi Isolated from Mangifera indica L. in Promoting Plant Growth

  • Kanyapat Sonsiam (Department of Microbiology, Faculty of Science, Chulalongkorn University) ;
  • Orlavanh Sonesouphap (Department of Microbiology, Faculty of Science, Chulalongkorn University) ;
  • Anyaporn Sangkaew (Department of Microbiology, Faculty of Science, Chulalongkorn University) ;
  • Pariyanuj Chulaka (Department of Horticulture, Faculty of Agriculture, Kasetsart University) ;
  • Prakitsin Sihanonth (Department of Microbiology, Faculty of Science, Chulalongkorn University) ;
  • Chulee Yompakdee (Department of Microbiology, Faculty of Science, Chulalongkorn University)
  • Received : 2024.02.01
  • Accepted : 2024.07.04
  • Published : 2024.09.28
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Abstract

Endophytic fungi have been shown to synthesize bioactive secondary metabolites, some of which promote plant growth through various mechanisms. In our previous study, endophytic fungi were isolated from mango trees (Mangifera indica L.). The present study examined fifty endophytic fungal isolates for mineral solubilization activity, ammonia production, and siderophore production. It was shown that these isolates could produce phytohormones indole-3-acetic acid and gibberellic acid, as well as inhibit plant pathogens, specifically Colletotrichum gloeosporioides and Lasiodiplodia theobromae. The results showed that all the isolated fungal endophytes exhibited various activities. Based on the findings, two fungal endophytes-Aureobasidium pullulans CY.OS 13 and Aspergillus tamarii CY.OS 144-were selected for dual inoculation in chili plants under pot-scale conditions to investigate their potential to improve growth-related traits such as seed germination, shoot and root length, biomass, and chlorophyll content. Seed treated with A. pullulans CY.OS 13 and/or A. tamarii CY.OS 144 showed a significant (p < 0.05) increase in seed germination and growth parameters of chili plants grown under pot-scale conditions. Particularly, chili plants whose seeds were injected with a combination of the two selected endophytic fungi showed the highest plant development traits. Therefore, the selected endophytic fungi have the potential to be used as biofertilizers, especially when combined. They could eventually replace chemical fertilizers because they are environmentally friendly, beneficial to humans, and can even promote sustainable agriculture.

Keywords

Acknowledgement

This research was supported by the Chulalongkorn University's Graduate Scholarship Program for ASEAN or Non-ASEAN for OS, the Chulalongkorn University Graduate School Scholarship in Commemoration of the 72nd Anniversary of His Majesty King Bhumibol Adulyadej and the 90th Anniversary of the Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund) for KS.

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