The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Investigation of Antimicrobial Compounds Produced by Endolichenic Fungi in Different Culture Media

  • Jaycee Augusto G. Paguirigan (Korean Lichen Research Institute, Sunchon National University) ;
  • Eunah Jeong (College of Pharmacy and Drug Information Research Institute, Sookmyung Women's University) ;
  • Kyo Bin Kang (College of Pharmacy and Drug Information Research Institute, Sookmyung Women's University) ;
  • Jae-Seoun Hur (Korean Lichen Research Institute, Sunchon National University) ;
  • Wonyong Kim (Korean Lichen Research Institute, Sunchon National University)
  • 투고 : 2024.06.06
  • 심사 : 2024.07.24
  • 발행 : 2024.10.01
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초록

Continuous use of synthetic fungicides has led to explosive emergence of fungicide-resistant microbes. Therefore, there are urgent needs for environmentally friendly antimicrobial agents with novel modes of action. This study investigated endolichenic fungi (ELF) as a source of antimicrobial compounds against various plant pathogens. We utilized an One Strain MAny Compounds (OSMAC) approach to enhance the chemical diversity of fourteen ELF. This involved cultivation of ELF in four growth media and subsequently assessing antimicrobial activities of culture extracts. Nearly half of the culture extracts exhibited antimicrobial activity against a Gram-positive bacterium, but showed minimal activity against Gram-negative bacteria tested. Notably, culture extracts from two ELF, Chaetomium globosum and Nodulisporium sp., demonstrated significant inhibitory effects against plant pathogenic fungi. LC-MS/MS-based metabolome profiling confirmed the presence of known bioactive compounds like cyclic dipeptides and chaetoglobosins. These findings highlight the effectiveness of combining OSMAC and metabolomics for identifying antimicrobial agents for agricultural use.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. NRF-2022R1C1C2004118 to W.K.) and the Korean National Research Resource Center Program (No. NRF-2017M3A9B8069471 and NRF-2021K2A9A1A06086000 to J.-S.H.). We thank the Korean Agricultural Culture Collection (KACC) for providing the phytopathogenic bacteria and fungi.

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