The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Capsicum annuum NAC4 (CaNAC4) Is a Transcription Factor with Roles in Biotic and Abiotic Stresses

  • Guogeng Jia (Department of Applied Biology, Chungnam National University) ;
  • Khaing Shwe Zin Thinn (Department of Applied Biology, Chungnam National University) ;
  • Sun Ha Kim (Department of Applied Biology, Chungnam National University) ;
  • Jiyoung Min (Department of Applied Biology, Chungnam National University) ;
  • Sang-Keun Oh (Department of Applied Biology, Chungnam National University)
  • 투고 : 2024.07.22
  • 심사 : 2024.08.29
  • 발행 : 2024.10.01
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초록

Transcription factors (TFs) regulate gene expression by binding to DNA. The NAC gene family in plants consists of crucial TFs that influence plant development and stress responses. The whole genome of Capsicum annuum shows over 100 NAC genes (CaNAC). Functional characteristics of the most CaNAC TFs are unknown. In this study, we identified CaNAC4, a novel NAC TF in C. annuum. CaNAC4 expression increased after inoculation with the pathogens, Xanthomonas axonopodis pv. vesicatoria race 3 and X. axonopodis pv. glycines 8ra, and following treatment with the plant hormones, salicylic acid and abscisic acid. We investigated the functional characteristics of the CaNAC4 gene and its roles in salt tolerance and anti-pathogen defense in transgenic Nicotiana benthamiana. For salt stress analysis, the leaf discs of wild-type and CaNAC4-transgenic N. benthamiana plants were exposed to different concentrations of sodium chloride. Chlorophyll loss was more severe in salt stress-treated wild-type plants than in CaNAC4-transgenic plants. To analyze the role of CaNAC4 in anti-pathogen defense, a spore suspension of Botrytis cinerea was used to infect the leaves. The disease caused by B. cinerea gradually increased in severity, and the symptoms were clearer in the CaNAC4-transgenic lines. We also investigated hypersensitive response (HR) in CaNAC4-transgenic plants. The results showed a stronger HR in wild-type plants after infiltration with the apoptosis regulator, BAX. In conclusion, our results suggest that CaNAC4 may enhance salt tolerance and act as a negative regulator of biotic stress in plants.

키워드

과제정보

This work was supported by the National Research Foundation of Korea (NRF) funded by the MEST (NRF-2017R1D1A1B03035692).

참고문헌

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