The Plant Pathology Journal

한국식물병리학회 (The Korean Society of Plant Pathology)
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Assessing Cold Plasma's Impact on Banana Growth and Fusarium Wilt Control

  • Priya Rajakumar (Institute of Biological Sciences, Faculty of Science, Universiti Malaya) ;
  • Nadiya Akmal Baharum (Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia) ;
  • Afiqah Insyirah Lutfi (Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia) ;
  • Najiah Mohd Sadali (Centre for Research in Biotechnology for Agriculture, Universiti Malaya) ;
  • Muhamad Shakirin Mispan (Institute of Biological Sciences, Faculty of Science, Universiti Malaya) ;
  • Lim Lian Kuang (Plasma Technology Research Centre, Department of Physics, Faculty of Science, Universiti Malaya) ;
  • Yap Seong Ling (Plasma Technology Research Centre, Department of Physics, Faculty of Science, Universiti Malaya) ;
  • Norzulaani Khalid (Universiti Malaya-Wales, Faculty of Arts and Science) ;
  • Nur Ardiyana Rejab (Institute of Biological Sciences, Faculty of Science, Universiti Malaya)
  • 투고 : 2024.05.30
  • 심사 : 2024.08.13
  • 발행 : 2024.10.01
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초록

Bananas (Musa spp.), which serve millions of people worldwide, face a serious threat from Fusarium wilt (FW) disease caused by Fusarium oxysporum f. sp. cubense (Foc). Developing disease-resistant varieties particularly through breeding is challenging due to banana's seedless nature (parthenocarpic). As an alternative, cold plasma (CP) technology, has the potential to be used for crop improvement. Our study demonstrates a favourable impact of CP on the growth performance of banana (Berangan cultivar, AAA) in terms of height, leaf number and stem diameter. CP-treated plants also displayed delayed disease progression as well as lower disease severity indicated by slightly lower value of leaf symptoms index and rhizome discoloration index compared to the control plants. Additionally, quantitative real-time polymerase chain reaction analysis revealed differential expression of several defence (PR1, WRKY22, PAL, and CEBiP) and growth (Cytochrome P450, NAC68, and CAT) related genes in CP-treated plants, particularly in conjunction with Foc infection. These findings shed light on the potential use of CP in managing FW in banana and offer insights into possible mechanism behind improved traits.

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과제정보

The authors thank Plant Biotechnology Incubator Unit (PBIU), Universiti Malaya, for providing tissue culturederived banana plantlets. We also thank Prof. Jennifer Ann Harikrishna, Dr. Purabi Mazumdar, and Puan Zarina for the invaluable assistance and the use of greenhouse facilities for our research. This research was supported by Impact Oriented Interdisciplinary Research Grant (IIRG006B19FNW), Universiti Malaya.

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