International Journal of Industrial Entomology and Biomaterials

  • 제48권3호
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  • Pages.107-113
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  • 2024
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  • 3022-7542(pISSN)
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  • 2586-4785(eISSN)
한국잠사학회 (Korean Society of Sericultural Science)
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Comparison of chemical and physical extraction methods of steamed-mature silkworm (Hongjam) protein

  • Ji Hae Lee (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jong Woo Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong-Wan Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sang Kuk Kang (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seul Ki Park (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyeok Gyu Kwon (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seong Ryul Kim (Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration)
  • 투고 : 2024.06.04
  • 심사 : 2024.06.25
  • 발행 : 2024.09.30
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초록

The efficiency of protein extraction from Hongjam, a steamed mature silkworm, was quantitatively evaluated using various chemical buffers and physical methods. This study considers the difficulty of protein extraction yield due to the high content of hydrophobic amino acids in Hongjam compared to 5th instar-3rd day silkworm larvae. Results indicated that urea buffer enhanced protein yield more effectively than RIPA buffer. Additionally, the application of physical methods such as microwave treatment to samples treated with RIPA buffer increased yields by up to 22%, achieving concentrations as high as 3.9 mg/mL. Circular dichroism (CD) analysis showed that proteins extracted with urea buffer retained their structural integrity, exhibiting deeper and more prominent peaks associated with random coil structure. In addition, physical methods such as vortexing, sonication, microwave and homogenization increased the extraction yield of larger molecules without altering protein structures, suggesting their potential scalability for industrial applications. These results demonstrate the critical role of selecting appropriate extraction methods to optimize the yield and functionality of proteins from Hongjam, with implications for its use in biotechnological applications and nutraceuticals.

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과제정보

This research was supported by the 'Research Program for Agriculture Science and Technology Development' (Grant No. RS-2022-RD010224) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

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