한국물환경학회지 (Journal of Korean Society on Water Environment)

  • 제40권4호
  • /
  • Pages.161-167
  • /
  • 2024
  • /
  • 2289-0971(pISSN)
  • /
  • 2289-098X(eISSN)
한국물환경학회 (Korean Society on Water Environment)
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비배양식 총세포수 평가를 통한 병물 위생관리 연구

Study on Hygiene Management of Bottled Water through Non-culture-based Total Cell Count

  • 정세영 (세종대학교 건설환경공학과) ;
  • 양민서 (세종대학교 건설환경공학과) ;
  • 이은수 (세종대학교 건설환경공학과) ;
  • 김상엽 (세종대학교 건설환경공학과) ;
  • 맹승규 (세종대학교 건설환경공학과)
  • Se Young Jeong (Department of Civil and Environmental Engineering, Sejong University) ;
  • Min Seo Yang (Department of Civil and Environmental Engineering, Sejong University) ;
  • Eun Su Lee (Department of Civil and Environmental Engineering, Sejong University) ;
  • Sang Yeob Kim (Department of Civil and Environmental Engineering, Sejong University) ;
  • Sung Kyu Maeng (Department of Civil and Environmental Engineering, Sejong University)
  • 투고 : 2024.04.19
  • 심사 : 2024.06.04
  • 발행 : 2024.07.30
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초록

The demand for bottled water in South Korea is steadily increasing, but there are challenges regarding water sources and violations of water quality standards. Consumers struggle to identify products that do not meet these standards, highlighting the need for improved water management. This study aims to investigate the use of flow cytometry to identify microbial behavior in bottled water. Twelve different bottled water brands were selected for this study. A novel non-culture-based analysis method called total cell count via flow cytometry was utilized, which is not commonly used to assess drinking water quality. This method was compared to conventional culture-based methods for heterotrophic plate count and E. coli experiments, in order to introduce new indicators for hygiene management. Adenosine triphosphate analysis was also conducted to assess cell activity, and total organic carbon was measured to determine the presence of organic matter. The total cell counts varied among the different bottled water brands. The adenosine triphosphate levels ranged from 37.1ng/L to 221.7ng/L, while the total organic carbon ranged from 0.4 to 0.6 mg/L. Furthermore, E. coli was not detected in any of the bottled waters, and with the exception of two cases, the levels of heterotrophic bacteria did not exceed the drinking water standard of 100 CFU/mL. This study demonstrated a correlation between total cell count and heterotrophic plate count, suggesting that non-culture-based analysis could be valuable in promptly assessing microbial contamination, in contrast to the conventional methods that require approximately 48 hours for incubation.

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과제정보

이 논문은 2022년도 세종대학교 교내연구비 지원에 의한 논문입니다.

참고문헌

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