Parasites, Hosts and Diseases

  • 제62권3호
  • /
  • Pages.342-350
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  • 2024
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  • 2982-5164(pISSN)
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  • 1738-0006(eISSN)
대한기생충학·열대의학회 (The Korea Society for Parasitology and Tropical Medicine)
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Proteome identification of common immunological proteins of two nematode parasites

  • Shin Ae Kang (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University) ;
  • Hak Sun Yu (Department of Parasitology and Tropical Medicine, School of Medicine, Pusan National University)
  • 투고 : 2024.04.05
  • 심사 : 2024.06.11
  • 발행 : 2024.08.31
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초록

Although helminth parasites have different life cycles, their hosts share similar immune responses involving Th2 cell-type. Here, we extracted proteins from the larvae of Anisakis simplex complex and Trichinella spiralis to identify common and specific antigens (or allergens) associated with the Th2 immune response. We performed two-dimensional electrophoresis analysis and Matrix-assisted laser desorption ionization-time of flight/time of flight (MALDI-TOF/TOF) experiments. We found 13 potentially immunogenic proteins, which included 5 spots specific to T. spiralis and 8 common to T. spiralis and A. simplex, by tandem mass spectrometry. These molecules were identified structurally as actin, tropomyosin, col cuticle N domain-containing protein, and heat shock proteins. We also identified molecules related to parasite-host immune modulation and interactions. Our results may contribute to reveal potential roles of immunological proteins in parasite-derived immune modulation.

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과제정보

This work was supported by a 2-Year Research Grant of Pusan National University.

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