Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Evaluation of a Compact Dry Method for Enumerating Bacteria in Contaminated Foods

식품 오염 미생물 분석을 위한 컴팩트 드라이법 평가

  • Soo-Jin Jung (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Sangha Han (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • June Gu Kang (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Min Su Song (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Hyewon Song (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Harim Lee (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Jisu Yu (Department of Food Safety and Regulatory Science, Chung-Ang University) ;
  • Kyung Ok Lee (Department of Food Science and Biotechnology, Chung-Ang University) ;
  • Sang-Do Ha (Department of Food Science and Biotechnology, Chung-Ang University)
  • 정수진 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 한상하 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 강준구 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 송민수 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 송혜원 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 이하림 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 유지수 (중앙대학교 일반대학원 식품공학부 식품안전규제과학과) ;
  • 이경옥 (중앙대학교 일반대학원 식품공학부 식품생명공학과) ;
  • 하상도 (중앙대학교 일반대학원 식품공학부 식품생명공학과)
  • Received : 2024.07.08
  • Accepted : 2024.08.20
  • Published : 2024.08.30
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Abstract

The present study aimed to compare the accuracy of the compact dry and culture plate methods for natural flora (yeasts/molds, coliforms, and total bacterial count) and artificial inoculum (Escherichia coli) in dosirak (meat-based), meal kits (seafood-based), and Doenjang (traditional food). Compact dry TC, EC, CF, and YMR were compared with culture plate methods using a suitable medium for each bacterium. The total bacterial count, coliforms, yeasts/molds, and E. coli were assessed with 3M Petrifilm (aerobic bacterial, coliform, yeast/mold, and E. coli count plates) using culture plate methods. Analysis of the recovery rates of target microorganisms in the three food samples showed that the compact dry method for total bacteria, coliforms, and E. coli exhibited recovery capabilities equivalent to those of the culture plate and Petrifilm methods, with no significant differences (P>0.05). Overall, compact dry TC, CF, and EC showed a good correlation between the methods used in this study, indicating rapid and convenient microbial enumeration by saving time and requiring less space.

식품공전 시험법은 식품기초규격 적부 판정, 수거검사의 적부판정, 수입식품의 적부판정 등의 판단근거가 되기 때문에 식품산업에서 매우 중요하다. 본 연구는 도시락, 밀키트, 된장에 오염된 일반세균, 대장균군, 효모, 곰팡이, 대장균을 분리하기 방법으로 사용되는 평판배지법, Petrifilm법, Compact Dry법을 이용해 각각의 세균을 분리하였고, 회수율을 비교하였다. 식품 내 자연균총(일반세균, 효모/곰팡이, 대장균군)검출은 평판배지 PCA, PDA, DCLA와 Petrifilm AC, YM, CC의 성능을 Compact Dry TC, YMR, CF와 비교하였다. 인위접종한 대장균(E. coli)의 경우는 평판배지 EMB, Petrilfilm EC, Compact Dry EC의 검출능을 비교하였다. 본 실험결과, 일반세균, 대장균군, 대장균의 검출에서 Compact Dry법은 기존의 평판배지법 및 건조배지법과 비교하여 식품 내 각각의 세균수를 계수하는데 유의적 차이를 보이지 않았다(P>0.05). 또한, 평판배지법과 Compact Dry간의 상관관계 및 Petrifilm과 Compact Dry간의 상관관계 역시 1에 가까운 높은 값을 확인하였다. Compact Dry법은 미생물 분석을 위해 배지 준비 과정이 필요 없으며, 접종 후 자체 확산이 가능하여 사용하기 쉽고, 공간을 효율적으로 사용할 수 있으므로 기존의 배지를 사용하는 방법에 비해 많은 장점을 제공하였다. 따라서 식품 중 일반세균, 대장균군, 대장균의 검출을 위한 Compact Dry법(Compact Dry TC, CF, EC)은 기존의 식품공전 상 등재된 다른 건조필름법을 대체 가능한 것으로 판단된다.

Keywords

References

  1. Korea Legislation Research Institute (KLRI), (2024, July 8). A study on systematization of food safety lows. Retrieved from https://www.klri.re.kr:9443/handle/2017.oak/5136
  2. Wilson, I.G., Gilmour, A., Cooper, J.E., Bjourson, A.J., Harvey, J.A., A non-isotopic DNA hybridization assay for the identification of Staphylococcus aureus isolated from foods. Int. J. Food Microbiol., 22, 43-54 (1994). https://doi.org/10.1016/0168-1605(94)90006-X
  3. Ministry of food and drug safety (MFDS), (2024, July 8). Korean food code. Retrieved from http://www.foodsafetykorea.go.kr/foodcode/01_01.jsp
  4. Teramura, H., Iwasaki, M., Ogihara, H., Evaluation of the quantitative dry culture method (SanitakunTM SA) for the enumeration of Staphylococcus aureus in artificially contaminated food samples. Biocontrol Sci., 20, 297-301 (2015). https://doi.org/10.4265/bio.20.297
  5. Park, J.H., Wang, X.Y., Lee, J.S., Kim, M., Evaluation of dry medium (Sanita-Kun) for enumeration of coliforms and Escherichia coli in milk, ice cream, ham, and codfish fillet. Food Sci. Biotechnol., 21, 1789-1793 (2012). https://doi.org/10.1007/s10068-012-0240-9
  6. Mizuochi, S., Nelson, M., Baylis, C., Jewell, K., Green, B., Limbum, R., Fernandez, M.C., Salfinger, Y., Chen, Y., Matrix extension study: validation of the Compact Dry TC method for enumeration of total aerobic bacteria in selected foods. J. AOAC. Int., 99, 461-468 (2016). https://doi.org/10.5740/jaoacint.15-0267
  7. Mizuochi, S., Nelson, M., Baylis, C., Green, B., Jewell, K., Monadjemi, F., Chen, Y., Salfinger, Y., Fernandez, M.C., Matrix extension study: validation of the Compact Dry EC method for enumeration of Escherichia coli and non-E. coli coliform bacteria in selected foods. J. AOAC. Int., 99, 451-460 (2016). https://doi.org/10.5740/jaoacint.15-0268
  8. Mizuochi, S., Nelson, M., Matrix extension study: validation of Compact Dry YM for enumeration of yeast and mold in selected foods. J. AOAC. Int., 99, 695-704 (2016). https://doi.org/10.5740/jaoacint.16-0059
  9. Leininger, D.J., Roberson, J.R., Elvinger, F., Use of eosin methylene blue agar to differentiate Escherichia coli from other gram-negative mastitis pathogens. J. Vet. Diagn. Invest., 13, 273-275 (2001). https://doi.org/10.1177/104063870101300319
  10. Gehm, H.W., Heukelekian, H., Eosin methylene blue agar for rapid direct count of E. coli. Am. J. Public Health Nations Health., 25, 920-923 (1935). https://doi.org/10.2105/AJPH.25.8.920
  11. Seong, J.Y., Park, M.J., Kwon, K.H., Oh, S.W., Combined effect of cold plasma and UV-C against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on fresh-cut lettuce. J. Food Hyg. Saf., 32, 64-69 (2017). https://doi.org/10.13103/JFHS.2017.32.1.64
  12. Ministry of food and drug safety (MFDS), (2024, July 8). Food code. Retrieved from http://www.foodsafetykorea.go.kr/portal/safefoodlife/food/foodRvlv/foodRvlv.do?menu_no=980&m enu_grp=MENU_GRP01.
  13. Ginn, R.E., Packard, V.S., Fox, T.L., Enumeration of total bacteria and coliforms in milk by dry rehydratable film methods: collaborative study. J. Assoc. Off. Anal. Chem., 69, 527-531 (1986).
  14. Mizuochi, S., Kodaka, H., Evaluation of dry sheet medium culture plate (Compactdry TC) method for determining numbers of bacteria in food samples. J. Food. Prot., 63, 665-667 (2000). https://doi.org/10.4315/0362-028X-63.5.665
  15. Park, H.K., Evaluation of dry rehydratable film method for detection of coliform bacteria and Escherichia coli. Korean J. Food Nutr., 22, 696-700 (2009).
  16. Beuchat, L.R., Nail, B.V., Brackett, R.E., Fox, T.L., Comparison of the PetrifilmTM yeast and mold culture film method to conventional methods for enumerating yeasts and molds in foods. J. Food Prot., 54, 443-447 (1991). https://doi.org/10.4315/0362-028X-54.6.443
  17. Kim, K.S., Bae, E.K., Ha, S.D., Park, Y.S., Mok, C.K., Hong, K.P., Kim, S.P., Park, J., Evaluation of dry rehydratable film method for enumeration of microorganisms in Korean traditional foods. J. Food Hyg. Saf., 19, 209-216 (2004).
  18. Hosokawa, S., Kodaka, H., Efficacy of Compact Dry EC for coliform detection in seafood. Jpn. J. Food Microbiol., 27, 80-85 (2010). https://doi.org/10.5803/jsfm.27.80