Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Comparing Molecular Weight Dependent Absorption Rates of Collagen in Oral Mucosal and Epidermis/dermis Tissue Models

  • Ji Yoon Hong (Department of Food and Nutrition, Hannam University) ;
  • Areum Cha (Institute of Functional Foods, EVERIT Co. Ltd.) ;
  • Gi Jung Kim (Department of Food and Nutrition, Hannam University) ;
  • Yelim Jang (Department of Food and Nutrition, Hannam University) ;
  • Jung-Yoon Lee (Department of Chemistry and Food Science, Framingham State University) ;
  • Emmanouil Apostolidis (Department of Chemistry and Food Science, Framingham State University) ;
  • Tae Yang Kim (Institute of Functional Foods, EVERIT Co. Ltd.) ;
  • Young-In Kwon (Department of Food and Nutrition, Hannam University)
  • Received : 2024.06.24
  • Accepted : 2024.07.05
  • Published : 2024.08.30
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Abstract

Collagen, as an indicator of skin health, has been developed and used for various purposes. The development of an optimized collagen product suitable for use has become an important research field as the consumption of collagen increases. In particular, various efforts are being made to increase its absorption rate. In this study, the transdermal and oral epithelial cell permeabilities of various molecular weight collagen products sold in Korea were compared using a Franz diffusion cell system. The collagen absorption rate of oral mucosal tissue compared to skin epidermis/dermis tissue was significantly higher than that of collagen at M.W. 500 and 1,000 (approximately 10 times and 2 times higher, respectively). Additionally, collagen with a molecular weight of 500 Da increased the absorption rates by 2-3 times compared with products with a molecular weight of 1,000. Collagen with a molecular weight of 500 Da showed the highest Cmax and AUCt values, and all parameters in the oral mucosal cell test group were higher than those in the skin epidermis/dermis cells. Our findings suggest an increased absorption rate through oral mucosal cells rather than skin absorption, confirming that low molecular weight collagen is a major factor increasing the absorption rate.

최근 국내외 화장품과 식품산업에서 다양하게 사용되어지고 있는 콜라겐 단백질 제품은 점차 그 용도와 특성에 따라 보다 고도화, 기능화 되어 가고 있다. 피부 건강의 지표인 콜라겐은 다양한 용도로 개발되어 사용되고 있으며, 콜라겐의 소비가 증가함에 따라 용도에 적합한 최적화된 콜라겐 제품의 개발이 중요한 연구 분야이다. 특히 여러 기업과 연구기관들에 의해서 콜라겐의 흡수율을 높이기 위한 다양한 노력이 이루어지고 있다. 따라서 본 연구에서는 프란즈(Franz) 세포 시스템을 이용하여 국내에서 판매되는 다양한 분자량별 콜라겐 제품의 경피 및 구강상피세포 투과성을 비교하였다. 그 결과, 피부 표피/진피 조직과 비교하여 구강점막 조직의 콜라겐 흡수율이 분자량 500달톤과 1,000달톤의 경우 모두 통계적으로 유의하게(각각 약 10배, 2배) 높은 것으로 확인되었다. 또한, 분자량별 구강점막 조직 흡수율을 비교한 결과, 분자량 500달톤의 콜라겐이 분자량 1,000달톤 제품에 비해 흡수율이 2-3배 증가함을 확인하였다. 분자량 500달톤의 경우 Cmax와 AUCt 값이 가장 높게 나타났으며, 피부 표피/진피 세포에 비해 구강점막세포 시험군의 모든 지표가 높은 것으로 나타났다. 본 연구 결과는 피부 흡수보다는 구강 점막 세포를 통한 콜라겐의 흡수방법이 콜라겐 체내 흡수증가에 유효한 수단임을 시사하며, 분자량 1,000달톤 이하에서도 보다 더 작은 500달톤의 저분자 콜라겐의 흡수율이 증가되는 것으로 보아 콜라겐의 분자량이 흡수율 증가의 주요한 요소임을 확인할 수 있었다.

Keywords

Acknowledgement

This research was financially supported by the Ministry of SMEs and Startup, Korea, under the "Regional Specialized Industry Development Program (Regional Star Industry)" supervised by the Korea Institute for Advancement of Technology (KIAT) (S2906741).

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