Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Study on the Anti-bacterium, Antioxidant and Anti-inflammatory Effect of Calystegia pubescens Lindl. Extracts

메꽃(Calystegia pubescens Lindl.) 추출물의 항균, 항산화 및 항염증 효과에 관한 연구

  • Ji-A Byeon (Department of Cosmetic Science, Kyungsung University) ;
  • Un-Gyo Shin (Department of Cosmetic Science, Kyungsung University) ;
  • Ye-Jin Jang (Department of Cosmetic Science, Kyungsung University) ;
  • Su-Bin Hwang (Department of Cosmetic Science, Kyungsung University) ;
  • Seon-A Lee (Department of Cosmetic Science, Kyungsung University) ;
  • Gayeon Kim (Department of Cosmeceutical Science, Kyungsung University) ;
  • Jin-Tae Lee (Department of Cosmetic Science, Kyungsung University) ;
  • Ildae Song (Department of Pharmaceutical Science and Technology, Kyungsung University) ;
  • Yong-Jin Kwon (Department of Cosmetic Science, Kyungsung University)
  • 변지아 (경성대학교 화장품학과) ;
  • 신운교 (경성대학교 화장품학과) ;
  • 장예진 (경성대학교 화장품학과) ;
  • 황수빈 (경성대학교 화장품학과) ;
  • 이선아 (경성대학교 화장품학과) ;
  • 김가연 (경성대학교 화장품약리학과) ;
  • 이진태 (경성대학교 화장품학과) ;
  • 송일대 (경성대학교 제약공학과) ;
  • 권용진 (경성대학교 화장품학과)
  • Received : 2024.05.17
  • Accepted : 2024.06.26
  • Published : 2024.06.30
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Abstract

Calystegia pubescens Lindl. (C. pubesens) is a native Korean herb that has been traditionally used for its diuretic, fatigue, and blood sugar-lowering effects. In previous studies, C. pubesens has shown antioxidant and whitening effects, but research on its antibacterial and anti-inflammatory properties is limited, and its potential as functional materials is lacking. In this study, we investigated the antibacterial, antioxidant, and anti-inflammatory effects of leaf (CPL) and stem (CPS) extracts of C. pubesens extracted with 70% ethanol. When the anti-bacterial effect was confirmed, CPL showed superior anti-bacterial effect than CPS for C. acne, S. aureus, E. coli and P. aeruginosa. Additionally, CPL exhibited significantly higher antioxidant activity compared to CPS, as demonstrated by DPPH and ABTS+ radical scavenging assays. Therefore, CPL showed superior anti-bacterial and antioxidant effects compared to CPS. Based on these results, further investigation was conducted to confirm the anti-inflammatory effect of CPL using RAW264.7 macrophages. Nitric oxide (NO) produced by lipopolysaccharide (LPS) stimulation was significantly reduced by CPL treatment in a dose-dependent manner. Additionally, the protein and mRNA expression of iNOS was reduced by CPL in LPS-indueced RAW264.7 cells, which was consistent with NO production. In conclusion, this study confirmed that CPL has superior anti-bacterial and antioxidant effects compared to CPS, and demonstrated anti-inflammatory effect of CPL via inhibiting iNOS expression and NO production induced by LPS. Based on the result, we suggest the potential of CPL as a valuable functional materials.

Calystegia pubescens Lindl. (C. pubesens)는 전통적으로 이뇨, 피로 및 혈당 강하 효과로 사용되어 온 한국 토종 식물이다. C. pubesens는 이전 연구에서 항산화 및 미백 효과를 보였지만 항균 및 항염증 특성에 대한 연구가 제한적이고 기능성 소재로서의 입증이 부족하다. 본 연구에서는 70% 에탄올로 추출한 C. pubesens의 잎(CPL)과 줄기(CPS) 추출물의 항균, 항산화 및 항염증 효과를 조사했다. CPL은 CPS보다 여드름균, 황색포도상구균, 대장균과 녹농균에 대해 우수한 항균 효과를 보였다. 또한 CPL은 CPS보다 DPPH 및 ABTS+ 라디칼 소거 분석에서 더 높은 항산화 활성을 나타냈다. 따라서 CPL은 CPS에 비해 우수한 항균 및 항산화 효과를 보였다. 이러한 결과를 바탕으로 RAW264.7 대식세포를 이용한 CPL의 항염증 효과를 확인하기 위한 추가 연구를 진행하였다. 지질다당류(LPS) 자극에 의해 생성된 산화질소(NO)는 CPL에 의해 감소되었다. 또한, LPS에 의해 증가된 iNOS의 단백질 및 mRNA 발현은 CPL에 의해 감소되었으며, 이는 NO 생산과 동일한 경향을 나타내었다. 결론적으로 본 연구는 CPL이 CPS에 비해 우수한 항균 및 항산화 효과를 가지고 있음을 확인하였으며, LPS에 의해 유도된 iNOS 발현 및 NO 생성 억제를 통해 CPL의 항염증 효과를 입증했다. 본 연구 결과를 바탕으로 CPL의 기능성 소재로서의 가능성을 제시한다.

Keywords

Acknowledgement

본 연구는 교육부의 지방대학 활성화 사업비를 지원받아 수행되었다.

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