Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Efficacy of Bambusae Caulis in Liquamen to protect intestinal epithelial cells via Nrf2 activation

죽력의 Nrf2 활성화를 통한 장상피세포 보호 효능

  • Jae Min Kim (College of Korean Medicine, Dongshin University) ;
  • Ji Hye Yang (College of Korean Medicine, Dongshin University)
  • 김재민 (동신대학교 한의과대학 한의예과) ;
  • 양지혜 (동신대학교 한의과대학 한의예과)
  • Received : 2023.11.11
  • Accepted : 2024.03.06
  • Published : 2024.05.31
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Abstract

Objectives : Intestinal epithelial cell damage is closely associated with various intestinal diseases, such as Inflammatory Bowel Disease (IBD), Celiac Disease and Gastroenteritis, and it plays a crucial role in the development and progression of intestinal diseases. Therefore, it is important to develop drugs that target protection of intestinal epithelial cells. Here, we aimed to investigated whether Bambusae Caulis in Liquamen (BCL) against t-BHP induced oxidative stress injury in human intestinal epithelial cells and to explore the underlying molecular mechanism. Methods : In this study, we performed MTT assay, measurement of ROS generation, and immunoblot analysis to determine the cytoprotective efficacy in HT29 cells (human colorectal adenocarinoma cell line with epithelial morphogy). Results : First, we checked that BCL was not cytotoxic up to concentration 30 ㎍/mL in HT29 cells. Then, we confirmed that BCL inhibited t-BHP-induced ROS and cell death. BCL also reversed the expression of proteins associated apoptosis. Next, to confirm the relationship between efficacy of BCL and Nrf2, we conducted experiments using siNrf2. Asresult, the effects of inhibiting ROS production and cell death of BCL was reversed by siNrf2. Conclusion : BCL prevents t-BHP-induced oxidative stress and apoptosis. And the efficacy of BCL is related to Nrf2 activation.

Keywords

Acknowledgement

This work is supported by the National Research Foundation of Korea (NRF), funded by a science and technology project that opens the future of the region (No. 2021-DD-UP-0380).

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